Leukocyte accumulation in graft blood vessels during self-limiting acute rejection of rat kidneys

Attenuation of Acute Rat Renal Allograft Rejection by Apolipoprotein E-Mimetic Peptide

“…Fatal acute rejection of DA kidneys was accompanied by reduced mRNA expression of ApoE in cells collected from allografts in comparison to the respective isografts or remained at the level of isografts after BN to LEW transplantation. In line with microarray data published before, 33 ApoE mRNA was elevated in intravascular leukocytes during reversible acute rejection of F344 kidneys ( Figure 1A). Although mRNA expression analyses were performed on the entire intravascular population of mononuclear leukocytes, ApoE was predominantly produced Apolipoprotein E mRNA expression was also detected in graft tissues and did not differ between healthy control kidneys, renal isografts, and allografts on day 4 after DA to LEW transplantation.…”

“…33 The data have been deposited in NCBI's Gene Expression Omnibus 36 and are accessible through GEO Series accession number GSE58156 at: http:// www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE58156.…”

“…[27][28][29][30] During acute rejection, numerous leukocytes, predominantly activated monocytes and T lymphocytes, accumulate in the vasculature of rat renal allografts. [31][32][33] They have been proposed to play a decisive role during organ rejection. To better characterize them and define potential factors involved in the resolution of acute graft rejection, the transcriptome of allograft leukocytes was compared to isograft leukocytes.…”

“…Apolipoprotein E was found among the genes, which were upregulated during reversible acute rejection. 33 Given the anti-inflammatory and immunomodulatory activities of ApoE, we hypothesized that ApoE is specifically induced during reversible but not during fatal acute rejection and that ApoE can promote renal allograft survival. To test this hypothesis, we investigated the expression of ApoE in different experimental models of acute rejection and assessed the effect of ApoE (133-149) application on allograft survival.…”

Expression of Acetylcholine Receptors by Experimental Rat Renal Allografts

“…Of note, a severe acute rejection episode peaks around day 9 posttransplantation and spontaneously resolves [7–10]. Six months after transplantation, however, renal function is impaired and all histopathological hallmarks of CAI are detected in allografts [8, 9]. This model opens up the possibility to investigate the contribution of acute rejection to CAI, which is difficult in patients.…”