Lethal and sublethal effects of acetamiprid on Eisenia andrei: Behavior, reproduction, cytotoxicity and oxidative stress

Saggioro, Enrico Mendes; Santo, Danielli Gundes do Espírito; Júnior, Sidney Fernandes Sales; Hauser‐Davis, Rachel Ann; Correia, Fábio Veríssimo

doi:10.1016/j.ecoenv.2019.109572

Cited by 42 publications

(19 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of ACE individually or in combination with LASs resulted in decreased enzyme activity in the exposed groups. The inhibition of GST activity was proven on other test organisms (Eisenia andrei and Apis mellifera) at higher concentrations [77,78] and on D. magna as well after the exposition of other neonicotinoid and pesticide AIs [79,80]. The reduction of GST activity is probably caused by the overproduction of reactive oxygen species (ROSs) as a result of xenobiotic-induced oxidative stress, while the released ROS significantly delay or inhibit the oxidation of substrate [81].…”

Section: Toxicity Of Neonicotinoid Active Ingredients and Formulationmentioning

confidence: 99%

Neonicotinoids: Spreading, Translocation and Aquatic Toxicity

Mörtl

Vehovszky

Klátyik

et al. 2020

IJERPH

View full text Add to dashboard Cite

Various environmental and ecotoxicological aspects related to applications of neonicotinoid insecticides are assessed. Dosages of neonicotinoids applied in seed coating materials were determined and are compared to other applications (spray and granule). Environmental levels in soils and affecting factors in translocation are discussed. Excretion of neonicotinoids via guttation from coated maize seeds up to two months upon emergence, as well as cross-contamination of plants emerged from non-coated seeds or weeds nearby have been demonstrated. Contamination of surface waters is discussed in scope of a worldwide review and the environmental fate of the neonicotinoid active ingredients and the formulating surfactant appeared to be mutually affected by each other. Toxicity of neonicotinoid active ingredients and formulations on Daphnia magna completed with some investigations of activity of the detoxifying glutathione S-transferase enzyme demonstrated the modified toxicity due to the formulating agents. Electrophysiological results on identified central neurons of the terrestrial snail Helix pomatia showed acetylcholine antagonist (inhibitory) effects of neonicotinoid insecticide products, but no agonist (ACh-like) effects were recorded. These data also suggested different molecular targets (nicotinergic acetylcholine receptors and acetylcholine esterase enzyme) of neonicotinoids in the snail central nervous system.Before the approval of neonicotinoids in seed treatment, numerous insect pests were controlled with the use of in-furrow granular insecticides. Seed coating is regarded as a precise delivery method of pesticides, which is therefore more effective compared to other plant protection product (PPP) applications (e.g., spraying). Neonicotinoids persist long enough to provide an effective control on a broad spectrum of insects, which led to their increased adoption by growers. As for maize, an estimated annual average of 45% and 25% of the total crop cultivation in the US was treated with CLO and TMX, respectively (with annual maxima of 65% for CLO and 45% for TMX in any given year) [16], and corresponding average proportion of the use of seeds coated with CLO or TMX are 46%, 33%, 24% and 18% for sugar beet, cotton, sorghum and soybean, respectively [16]. In accordance with these maximum rates, nearly 100% of maize in the US and canola in Canada is cultivated from seeds coated with neonicotinoids [17] and often fungicides as well.One of the first signs of the environmental problem caused by neonicotinoids was detected in Germany in the spring 2008, when abrasion of active substance from treated seeds during sowing of maize led to mass poisonings incidents of bees [18]. Thereafter, substantial efforts were made to minimize the environmental impacts of these crop protection products. Improvements in seed coating technology (e.g., application of polymers) and modification of pneumatic planters have been introduced to reduce abrasion of the seed coating material during planting. Polymers in seed treatment not only i...

show abstract

Section: Toxicity Of Neonicotinoid Active Ingredients and Formulationmentioning

confidence: 99%

Neonicotinoids: Spreading, Translocation and Aquatic Toxicity

Mörtl

Vehovszky

Klátyik

et al. 2020

IJERPH

View full text Add to dashboard Cite

show abstract

“…This result was accordant with the acute toxicity test that earthworms prefer to stay on or near the surface of soil substrate (Section 3.1). These results show that avoidance behavior is an essential endpoint in toxicological assessment and can provide extrapolations at the ecosystem level, even at low doses (Saggioro et al 2019;Junior et al 2020).…”

Section: Acute Toxicitymentioning

confidence: 78%

Behavioural, Histopathological, Genetic and Organism-Wide Responses to Phenanthrene-Induced Oxidative Stress in Eisenia Fetida Earthworms in Natural Soil Microcosms

Shi

et al. 2021

Preprint

View full text Add to dashboard Cite

Phenanthrene (PHE) contamination not only changes the quality of soil environment but also threatens to the soil organisms. There is lack of focus on the eco-toxicity potential of contaminants in real soil in the current investigation. Here, we assessed the toxic effects of PHE on earthworms (Eisenia fetida) in natural soil matrix. PHE exhibited a relatively high toxicity to E. fetida in natural soil, with the LC50 determined to be 56.68 mg kg−1 after a 14-day exposure. Excessive ROS induced by PHE, leading to oxidative damage to biomacromolecules in E. fetida, including lipid peroxidation, protein carbonylation, and DNA damage. The antioxidant defense system (total antioxidant capacity, glutathione S-transferase, peroxidase, catalase, carboxylesterase, and superoxide dismutase) in E. fetida responded quickly to scavenge excess ROS and free radicals. Exposure to PHE resulted in earthworm avoidance responses (2.5 mg kg−1) and habitat function loss (10 mg kg−1). Histological observations indicated that the intestine, body wall, and seminal vesicle in E. fetida were severely damaged after exposure to high-dose PHE. Moreover, earthworm growth (weight change) and reproduction (cocoon production and the number of juvenile) were also inhibited after exposure to this pollutant. Furthermore, the integrated toxicity of PHE toward E. fetida at different doses and exposure times was assessed by the integrated biomarker response (IBR), which confirm that PHE is more toxic to earthworms in the high-dose and long-term exposure groups. Our results showed that PHE exposure induced oxidative stress, disturbed antioxidant defense system, and caused oxidative damage in E. fetida. These effects can trigger behavior changes and damaged histological structure, finally cause growth inhibition, genotoxicity, and reproductive toxicity in earthworms. The strength of this study is the comprehensive toxicity evaluation of PHE to earthworms and highlights the need to investigate the t eco-toxicity potential of exogenous environmental pollutants in a real soil environment.

show abstract

“…Another reference (Saggioro et al, 2019 ; RefID 14) reports on the outcomes of different experiments with the earthworm Eisenia andrei . One set‐up was an acute test with the earthworms exposed via contact to contaminated moist filter paper for up to 72 h (experiment 1).…”

Section: Environmentmentioning

confidence: 99%

Statement on the active substance acetamiprid

Hernandez-Jerez¹,

Adriaanse²,

Berny³

et al. 2022

EFS2

View full text Add to dashboard Cite

Acetamiprid is a pesticide active substance with insecticidal action currently under the third renewal (AIR3) of the Commission implementing regulation (EU) No 844/2012. Following concerns that this substance may pose high risks to humans and the environment, the French authorities asked the Commission to restrict its uses under Article 69 of Regulation (EC) No 1107/2009. To support this request, competent Authorities from France cited a series of literature papers investigating its hazards and/or exposure to humans and the environment. Consequently, the EFSA PPR Panel was mandated to advise on the likelihood that body of evidence would constitute proof of serious risks to humans or the environment. Therefore, the EFSA PPR Panel evaluated the likelihood of these studies indicating new or higher hazards and exposure to humans and the environment compared to previous EU assessments.A stepwise methodology was designed, including: (i) the initial screening; (ii) the data extraction and critical appraisal based on the principles of OHAT/NTP; (iii) the weight of evidence, including consideration of the previous EU assessments; (iv) the uncertainty analysis, followed, whenever relevant, by an expert knowledge elicitation process. For human health, no conclusive evidence of higher hazards compared to previous assessment was found for genotoxicity, developmental toxicity, neurotoxicity including developmental neurotoxicity and immunotoxicity. However, due to the lack of adequate assessment of the current data set, the PPR Panel recommends conducting an assessment of endocrine disrupting properties for acetamiprid in line with EFSA/ECHA guidance document for the identification of endocrine disruptors. For environment, no conclusive, robust evidence of higher hazards compared to the previous assessment was found for birds, aquatic organisms, bees and soil organisms. However, the potential of high inter-species sensitivity of birds and bees towards acetamiprid requires further consideration.

show abstract

Lethal and sublethal effects of acetamiprid on Eisenia andrei: Behavior, reproduction, cytotoxicity and oxidative stress

Cited by 42 publications

References 46 publications

Neonicotinoids: Spreading, Translocation and Aquatic Toxicity

Neonicotinoids: Spreading, Translocation and Aquatic Toxicity

Behavioural, Histopathological, Genetic and Organism-Wide Responses to Phenanthrene-Induced Oxidative Stress in Eisenia Fetida Earthworms in Natural Soil Microcosms

Statement on the active substance acetamiprid

Contact Info

Product

Resources

About