Lessons Learned from Bacterial Transport Research at the South Oyster Site

Scheibe, T. D.; Hubbard, Susan S.; Onstott, Tullis C.; DeFlaun, Mary F.

doi:10.1111/j.1745-6584.2011.00831.x

Cited by 22 publications

(17 citation statements)

References 88 publications

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“…Recent reviews have focused on the apparent discrepancy between rates of bacterial transport measured in laboratory columns versus field transport experiments, generally manifested as an apparent decrease in measured attachment rates with increasing scale of the experiment [ Foppen and Schijven , 2006; Dong et al , 2006; Pang et al , 2008; Scheibe et al , 2011]. One proposed explanation is that there is a subpopulation of less adhesive bacteria that are not easily detected at the scale of a typical column experiment [ Bolster et al , 2000].…”

Section: Introductionmentioning

confidence: 99%

“…[4] Attachment rates for bacteria have been inferred under various conditions from aquifer-scale forced gradient and natural gradient tracer tests [Harvey et al, 1989;Bales et al, 1997;Knappett et al, 2012] or centimeter-scale column experiments [Harvey et al, 1993;Litton and Olson, 1993;Fitzpatrick and Spielman, 1973] using stained bacteria or latex microspheres with size and surface properties that are similar to bacteria. Recent reviews have focused on the apparent discrepancy between rates of bacterial transport measured in laboratory columns versus field transport experiments, generally manifested as an apparent decrease in measured attachment rates with increasing scale of the experiment [Foppen and Schijven, 2006;Dong et al, 2006;Pang et al, 2008;Scheibe et al, 2011]. One proposed explanation is that there is a subpopulation of less adhesive bacteria that are not easily detected at the scale of a typical column experiment [Bolster et al, 2000].…”

Section: Introductionmentioning

confidence: 99%

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Transport of E. coli in aquifer sediments of Bangladesh: Implications for widespread microbial contamination of groundwater

Feighery

Mailloux

Ferguson

et al. 2013

Water Resources Research

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Fecal bacteria are frequently found at much greater distances than would be predicted by laboratory studies, indicating that improved models that incorporate more complexity are might be needed to explain the widespread contamination of many shallow aquifers. In this study, laboratory measurements of breakthrough and retained bacteria in columns of intact and repacked sediment cores from Bangladesh were fit using a two-population model with separate reversible and irreversible attachment sites that also incorporated bacterial decay rates. Separate microcosms indicated an average first order decay rate of 0.03 log10 / day for free bacteria in both the liquid phase and bacteria attached to the solid phase. Although two-thirds of the column results could be well fit with a dual deposition site, single population model, fitting of one third of the results required a two-population model with a high irreversible attachment rate (between 5 and 60 hr-1) for one population of bacteria and a much lower rate (from 5 hr-1 to essentially zero) for the second. Inferred attachment rates for the reversible sites varied inversely with grain size (varying from 1 - 20 hr-1 for grain sizes between 0.1 and 0.3 mm) while reversible detachment rates were found to be nearly constant (approximately 0.5 hr-1). Field simulations based on the fitted two-population model parameters predict only a two-fold reduction in fecal source concentration over a distance of 10 m, determined primarily by the decay rate of the bacteria. The existence of a secondary population of bacteria with a low attachment rate might help explain the observed widespread contamination of tubewell water with E. coli at the field site where the cores were collected, as well as other similar sites.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transport of E. coli in aquifer sediments of Bangladesh: Implications for widespread microbial contamination of groundwater

Feighery

Mailloux

Ferguson

et al. 2013

Water Resources Research

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“…To test our methodology, we first applied the method to a synthetic infiltration study in a heterogeneous soil in which the two‐dimensional soil moisture distribution is exactly known (Kowalsky et al, 2005). This was followed by a real‐world study using geophysical field data from the South Oyster Bacterial Transport Site in Virginia (Hubbard et al, 2001; Linde et al, 2008; Scheibe et al, 2011). Throughout this study, we compared the soil moisture uncertainty estimates derived with our Bayesian methodology with those from a classical deterministic Occam's inversion and first‐order uncertainty analysis.…”

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confidence: 99%

Distributed Soil Moisture from Crosshole Ground‐Penetrating Radar Travel Times using Stochastic Inversion

Linde

Vrugt

2013

Vadose Zone Journal

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Linde, N., and J. A. Vrugt, 2013, Distributed soil moisture from crosshole ground-penetrating radar travel times using stochastic inversion. Vadose Zone Journal. 12, doi:10.2136 2 AbstractGeophysical methods offer several key advantages over conventional subsurface measurement approaches, yet their use for hydrologic interpretation is often problematic.Here, we introduce theory and concepts of a novel Bayesian approach for high-resolution soil moisture estimation using traveltime observations from crosshole Ground Penetrating Radar (GPR) experiments. The recently developed Multi-try DiffeRential Evolution Adaptive Metropolis with sampling from past states, MT-DREAM (ZS) is being used to infer, as closely and consistently as possible, the posterior distribution of spatially distributed vadose zone soil moisture and/or porosity under saturated conditions. Two differing and opposing model parameterization schemes are being considered, one involving a classical uniform grid discretization and the other based on a discrete cosine transformation (DCT). We illustrate our approach using two different case studies involving geophysical data from a synthetic water tracer infiltration study and a real-world field study under saturated conditions. Our results demonstrate that the DCT parameterization yields the most accurate estimates of distributed soil moisture for a large range of spatial resolutions, and superior MCMC convergence rates.In addition, DCT is admirably suited to investigate and quantify the effects of model truncation errors on the MT-DREAM (ZS) inversion results. For the field example, lateral anisotropy needs to be enforced to derive reliable soil moisture variability. Our results also demonstrate that the posterior soil moisture uncertainty derived with the proposed Bayesian procedure is significantly larger than its counterpart estimated from classical smoothnessconstrained deterministic inversions.

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“…Scheibe et al . () summarized the various methods that were employed to track bacteria being advected through a shallow sandy aquifer in Oyster, Virginia, USA.…”

Section: Introductionmentioning

confidence: 99%

“…Other tracking methods include radiolabeling (Jewett et al, 1995), stable isotope labeling (Holben & Ostrom, 2000) and immunomagnetic labeling (Sherwood et al, 2003;Olson, 2004;Olson et al, 2004), and engineered auto-fluorescence (Trevors et al, 1990), but tend to be more cost-and labor-intensive and (or) may be unsuitable for some field sites because of permitting issues. Scheibe et al (2011) summarized the various methods that were employed to track bacteria being advected through a shallow sandy aquifer in Oyster, Virginia, USA.…”

Section: Introductionmentioning

confidence: 99%

Impact of fluorochrome stains used to study bacterial transport in shallow aquifers on motility and chemotaxis of Pseudomonas species

Toepfer

Ford

Metge

et al. 2012

FEMS Microbiol Ecol

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One of the most common methods of tracking movement of bacteria in groundwater environments involves a priori fluorescent staining. A major concern in using these stains to label bacteria in subsurface injection‐and‐recovery studies is the effect they may have on the bacterium's transport properties. Previous studies investigated the impact of fluorophores on bacterial surface properties (e.g. zeta potential). However, no previous study has looked at the impact of fluorescent staining on swimming speed and chemotaxis. It was found that DAPI lowered the mean population swimming speed of Pseudomonas putida F1 by 46% and Pseudomonas stutzeri by 55%. DAPI also inhibited the chemotaxis in both strains. The swimming speeds of P. putida F1 and P. stutzeri were diminished slightly by CFDA/SE, but not to a statistically significant extent. CFDA/SE had no effect on chemotaxis of either strain to acetate. SYBR® Gold had no effect on swimming speed or the chemotactic response to acetate for either strain. This research indicates that although DAPI may not affect sorption to grain surfaces, it adversely affects other potentially important transport properties such as swimming and chemotaxis. Consequently, bacterial transport studies conducted using DAPI are biased to nonchemotactic conditions and do not appear to be suitable for monitoring the effect of chemotaxis on bacterial transport in shallow aquifers.

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Lessons Learned from Bacterial Transport Research at the South Oyster Site

Cited by 22 publications

References 88 publications

Transport of E. coli in aquifer sediments of Bangladesh: Implications for widespread microbial contamination of groundwater

Transport of E. coli in aquifer sediments of Bangladesh: Implications for widespread microbial contamination of groundwater

Distributed Soil Moisture from Crosshole Ground‐Penetrating Radar Travel Times using Stochastic Inversion

Impact of fluorochrome stains used to study bacterial transport in shallow aquifers on motility and chemotaxis of Pseudomonas species

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