Lentiviral shRNA knockdown of Charcot-Leyden Crystal Protein/Galectin-10 Expression in Developing Human Eosinophils Impairs Granulogenesis

Doyle, Christine B.; Ackerman, Steven J.

doi:10.1016/j.jaci.2008.12.1045

Cited by 2 publications

(4 citation statements)

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“…In addition to localization in eosinophils (and basophils), recent studies have reported intra-cytoplasmic CLC expression in human CD4 + CD25 + Foxp3 + regulatory T cells (Treg) [30]. Its expression is not only necessary for limiting Treg-proliferation and maintaining Treg anergy, but is also crucial for maintaining Treg-mediated immuno-suppression of co-cultured CD4 + T lymphocytes [30]. The protein is constitutively expressed in the Tregs and is not secreted nor released under the described experimental conditions [30].…”

Section: Charcot-leyden Crystals In Regulatory T Cellsmentioning

confidence: 99%

“…Its expression is not only necessary for limiting Treg-proliferation and maintaining Treg anergy, but is also crucial for maintaining Treg-mediated immuno-suppression of co-cultured CD4 + T lymphocytes [30]. The protein is constitutively expressed in the Tregs and is not secreted nor released under the described experimental conditions [30]. Regulatory T cells play a crucial role in the maintenance of immunological selftolerance [30,31], the prevention of a variety of autoimmune and inflammatory diseases, and inhibit antitumor immune reactions by suppressing tumor-specific T cell responses [31].…”

Section: Charcot-leyden Crystals In Regulatory T Cellsmentioning

confidence: 99%

“…The protein is constitutively expressed in the Tregs and is not secreted nor released under the described experimental conditions [30]. Regulatory T cells play a crucial role in the maintenance of immunological selftolerance [30,31], the prevention of a variety of autoimmune and inflammatory diseases, and inhibit antitumor immune reactions by suppressing tumor-specific T cell responses [31]. Although little is known about the molecular mechanisms and proteins that contribute to the in vitro anergy and regulatory activity of Tregs [30], these findings offer implications for Charcot-Leyden crystal protein in their functional properties.…”

Section: Charcot-leyden Crystals In Regulatory T Cellsmentioning

confidence: 99%

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The Enigmatic Charcot-Leyden Crystal Protein (Galectin-10): Speculative Role(s) in the Eosinophil Biology and Function

Clarke¹

2015

J Clin Cell Immunol

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Eosinophilic inflammation in peripheral tissues is typically marked by the deposition of a prominent eosinophil protein, Galectin-10, better known as Charcot-Leyden crystal protein (CLC). Unlike the eosinophil's four distinct toxic cationic proteins and enzymes [major basic protein (MBP), eosinophil-derived neurotoxin (EDN), eosinophil cationic protein (ECP), and eosinophil peroxidase (EPO)], there is a paucity of information on the precise role of the crystal protein in the biology of the eosinophil. While its clinical significance at inflammatory foci remains highly speculative, its relative abundance (~10% of total eosinophil protein), as well as its dual nuclear and cytosolic localization is, however, suggestive of its biological and functional significance. In this article, we present a short review of the Charcot-Leyden crystal protein, specifically highlighting its most recently delineated modulatory role in regulatory T lymphocytes, and its speculative intracellular and extracellular role(s) in eosinophil function or associated inflammatory responses.

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Section: Charcot-leyden Crystals In Regulatory T Cellsmentioning

confidence: 99%

Section: Charcot-leyden Crystals In Regulatory T Cellsmentioning

confidence: 99%

Section: Charcot-leyden Crystals In Regulatory T Cellsmentioning

confidence: 99%

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The Enigmatic Charcot-Leyden Crystal Protein (Galectin-10): Speculative Role(s) in the Eosinophil Biology and Function

Clarke¹

2015

J Clin Cell Immunol

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“…30 CLC protein expression has been identified in human CD4+ CD25+ Foxp3+ regulatory T (Treg) cells. 7 Initially, CLCs were thought to be lysolecithin acylhydrolase, which has lysophospholipase activity and interacts with the enzyme lysophospholipase. 31 However, lysolecithin acylhydrolase has been renamed galectin-10 17 based on the sequence similarity to galectins and weak lactose-binding activity.…”

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confidence: 99%

Charcot–Leyden crystals: do they exist in veterinary species? A case report and literature review

et al. 2017

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The Charcot-Leyden crystal (CLC) is a major human eosinophil protein that readily crystallizes; these crystals are common in eosinophilic diseases. Although anecdotal existence of these crystals is known in veterinary pathology, definitive reports do not exist, to our knowledge. We identified eosinophilic crystals in a laryngeal myxosarcoma from a 2-y-old, spayed female, Labrador Retriever dog that were tentatively interpreted as CLCs. However, Ziehl-Neelsen acid-fast stain was negative, arguing against CLCs. The crystals stained red with Masson trichrome, precluding collagen. Periodic acid-Schiff and alcian blue were negative. The crystals stained positively with Okajima, and no myoglobin immunoreactivity was detected, supporting their identity as hemoglobin crystals. In the absence of a hematologic abnormality, these crystals were interpreted to be abnormal hemoglobin breakdown products. Protein sequence comparison was pursued to determine whether a protein similar to CLC exists in mammals. Only 3 nonhuman primate species, the Sumatran orangutan ( Pongo abelii), rhesus macaque ( Macaca mulatta), and cynomolgus monkey ( Macaca fascicularis), had a sequence similarity of >80%. Of the crystal-forming residues, 12 of 54 (22%) were different in the Sumatran orangutan and 15 of 54 (28%) were different in the Macaca spp., which may affect the crystallization process. The lack of reports of CLCs in nonhuman species and our results collectively suggest that CLCs are human-specific.

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Lentiviral shRNA knockdown of Charcot-Leyden Crystal Protein/Galectin-10 Expression in Developing Human Eosinophils Impairs Granulogenesis

Cited by 2 publications

References 0 publications

The Enigmatic Charcot-Leyden Crystal Protein (Galectin-10): Speculative Role(s) in the Eosinophil Biology and Function

The Enigmatic Charcot-Leyden Crystal Protein (Galectin-10): Speculative Role(s) in the Eosinophil Biology and Function

Charcot–Leyden crystals: do they exist in veterinary species? A case report and literature review

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