Lectins isolated from Korean mistletoe (Viscum album coloratum) induce apoptosis in tumor cells

Yoon, Tae‐Jong; Yoo, Yung Choon; Kang, Tae-Bong; Shimazaki, K.; Song, Seong Kyu; Lee, Kwan Hee; Kim, Seon Ho; Park, Choon Ho; Azuma, Ichiro; Kim, Jong Bae

doi:10.1016/s0304-3835(98)00300-0

Cited by 77 publications

(49 citation statements)

References 21 publications

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“…However, all groups showed comparable dimension of necrosis within the PTs and no correlation between diameter of necrosis and tumour vessels was evident. Thus, in our model no antiangiogenic effect of European ML-I was evidenced; however, differences in the effects on various tumour cells between Korean and European ML-I have already been shown (Yoon et al, 1999). Further correlation analyses revealed no correlation between vascular density and the number of LMs, or the weight of the PT, which shows that the period of 20 days of tumour growth and ML-I treatment might be too short to sufficiently model the complex mechanisms of the effects on vascularisation.…”

Section: Discussionmentioning

confidence: 48%

Low-dose mistletoe lectin-I reduces melanoma growth and spread in a scid mouse xenograft model

et al. 2007

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This study investigates the effects of mistletoe lectin-I (ML-I) on melanoma growth and spread in vivo. The human melanoma cell line MV3 was xenografted into severe combined immunodeficient mice and vehicle solution or purified ML-I was administered at 30, 150 and 500 ng per kg body weight (20 mice per group) daily. After 19 days, mice were killed, primary tumours (PTs) and lungs were dissected out, and tumour weights, number of lung metastases (LMs), number of tumour-infiltrating dendritic cells (DCs), and apoptosis rates in the melanoma cells and in the DCs were assessed. A 35% reduction of PT weight (P ¼ 0.03) and a 55% decrease in number of LMs (P ¼ 0.016) were evident for low-dose ML-I (30 ng kg À1 ) treatment but not for higher doses. Mistletoe lectin-I increased apoptosis rates in the melanoma cells of PTs at all doses, while no induction of apoptosis was noted in the LMs. Low-dose ML-I significantly increased the number of DCs infiltrating the PTs (Po0.0001) and protected DCs against apoptosis, while higher doses induced apoptosis in the DCs (Po0.01). Our results demonstrate that low-dose ML-I reduced melanoma growth and number of metastases in vivo, primarily due to immunomodulatory effects.

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Section: Discussionmentioning

confidence: 48%

Low-dose mistletoe lectin-I reduces melanoma growth and spread in a scid mouse xenograft model

et al. 2007

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“…The active CH2Cl2 soluble fraction (113 g) was chromatographed on the silica gel (12 × 75 cm, CH2Cl2：MeOH = 500：1 → 1：1) to give 14 fractions (Fr. [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Compound 1 (3 g) was obtained from Fr.…”

Section: Plant Material Extraction and Isolationmentioning

confidence: 99%

“…Mistletoe (Viscum album) is a semi-parasitic plant with a worldwide distribution in all over the world that grows on many kinds of trees, and its aqueous extracts have been used as anti-cancer agents for almost a century (9). Korean mistletoe (KM) is also used in traditional medicine to treat cancer, cardiovascular disease, and arthrosis (10), and studies of the biological effects of KM and of its chemical composition have demonstrated that the lectins of KM induce the apoptosis of cancer cells (11). Other studies have shown that they have immunomodulatory activity and augment antigen-specific cellular and humoral immune responses (12).…”

Section: Introductionmentioning

confidence: 99%

Anticoagulant activities of oleanolic acid via inhibition of tissue factor expressions

Lee

Yang²,

Ku³

et al. 2012

BMB Reports

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“…than European mistletoe lectins (EML: 60 kDa) against various cancer cell lines (Yoon et al, 1999). In immunological aspects, KML has been reported to positively modulate NK cell activity, antigen-specific antibody production, Th1/Th2 cell activation, and induction of cytolytic T lymphocyte activity and macrophage functions such as NO production (Yoon et al, 2003;Lyu and Park, 2006;Kang et al, 2008).…”

Section: Genementioning

confidence: 99%

“…KML was prepared by a method reported previously (Park et al, 1998;Yoon et al, 1999). Briefly, Korean mistletoe (Viscum album L. var.…”

Section: Preparation Of Kmlmentioning

confidence: 99%

Extracellular Signal-Regulated Kinase Is a Major Enzyme in Korean Mistletoe Lectin-Mediated Regulation of Macrophage Functions

Byeon¹,

Lee²,

Yu³

et al. 2009

Biomolecules and Therapeutics

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-Korean mistletoe lectin (KML) is the major component found in Viscum album var. (coloratum), displaying anti-cancer and immunostimulating activities. Even though it has been shown to boost host immune defense mechanisms, the regulatory roles of KML on the functional activation of macrophages have not been fully elucidated. In this study, regulatory mechanism of KML on macrophage-mediated immune responses was examined in terms of KML-mediated signaling event. KML clearly induced mRNA expression of tumor necrosis factor (TNF)-α, the generation of reactive oxygen species (ROS) and phagocytic uptake in RAW264.7 cells. All of these events were strongly suppressed by U0126, whereas TNF-α mRNA was not diminished by SB203580 and SP600125, indicating ERK as a central enzyme managing KML-induced up-regulation of macrophage functions. Indeed, KML strongly induced the phosphorylation of ERK in a time-dependent manner without altering its total level. Therefore, these data suggest that ERK may be a major signaling enzyme with regulatory property toward various KML-mediated macrophage responses.

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Lectins isolated from Korean mistletoe (Viscum album coloratum) induce apoptosis in tumor cells

Cited by 77 publications

References 21 publications

Low-dose mistletoe lectin-I reduces melanoma growth and spread in a scid mouse xenograft model

Low-dose mistletoe lectin-I reduces melanoma growth and spread in a scid mouse xenograft model

Anticoagulant activities of oleanolic acid via inhibition of tissue factor expressions

Extracellular Signal-Regulated Kinase Is a Major Enzyme in Korean Mistletoe Lectin-Mediated Regulation of Macrophage Functions

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