Leaf proteome pattern of two bread wheat varieties under water deficit stress conditions

Shayan, Soheila; Norouzi, Mehdi; Vahed, Mohammad Moghaddam; Mohammadi, Seyed Abolghasem; Toorchi, Mahmoud

doi:10.1016/j.cpb.2020.100146

Cited by 7 publications

(5 citation statements)

References 61 publications

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“…The alteration in photosynthesis of plants highly related to the Rubisco activity. It has been reported that drought stress had a harmful impact on the function of Rubisco in various plants, led to decrease of biosynthesis and degradation of subunits and finally diminution of photosynthesis ( Zhang et al., 2016 ; Shayan et al., 2020 ). In this study, subunits of Rubisco down-regulated which was agreement with our pervious study ( Pakzad et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Proteomics approach to investigating osmotic stress effects on pistachio

et al. 2023

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Osmotic stress can occur due to some stresses such as salinity and drought, threatening plant survival. To investigate the mechanism governing the pistachio response to this stress, the biochemical alterations and protein profile of PEG-treated plants was monitored. Also, we selected two differentially abundant proteins to validate via Real-Time PCR. Biochemical results displayed that in treated plants, proline and phenolic content was elevated, photosynthetic pigments except carotenoid decreased and MDA concentration were not altered. Our findings identified a number of proteins using 2DE-MS, involved in mitigating osmotic stress in pistachio. A total of 180 protein spots were identified, of which 25 spots were altered in response to osmotic stress. Four spots that had photosynthetic activities were down-regulated, and the remaining spots were up-regulated. The biological functional analysis of protein spots exhibited that most of them are associated with the photosynthesis and metabolism (36%) followed by stress response (24%). Results of Real-Time PCR indicated that two of the representative genes illustrated a positive correlation among transcript level and protein expression and had a similar trend in regulation of gene and protein. Osmotic stress set changes in the proteins associated with photosynthesis and stress tolerance, proteins associated with the cell wall, changes in the expression of proteins involved in DNA and RNA processing occur. Findings of this research will introduce possible proteins and pathways that contribute to osmotic stress and can be considered for improving osmotic tolerance in pistachio.

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Section: Discussionmentioning

confidence: 99%

Proteomics approach to investigating osmotic stress effects on pistachio

et al. 2023

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“…In both wheat and barley, the expression of Rubisco large subunit under water deficit condition decreased ( Chmielewska et al, 2016 , Michaletti et al, 2018 ). In the thylakoid membrane of rice, Rubisco plays a role in the defense and regulation of photosynthesis under moderate heat stress ( Shayan et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Differential expression gene/protein contribute to heat stress-responsive in Tetraena propinqua in Saudi Arabia

Alafari

Abd-Elgawad

2021

Saudi Journal of Biological Sciences

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Within their natural habitat, plants are subjected to abiotic stresses that include heat stress. In the current study, the effect of 4 h, 24 h, and 48 h of heat stress on Tetraena propinqua ssp. migahidii seedling’s protein profile and proteomic analyses were investigated. Total soluble protein SDS-PAGE (Sodium dodecyl sulfate–polyacrylamide gel electrophoresis) profile showed 18-protein bands, the newly synthesized protein band (with molecular weights 86.5, 30.2 and 31.4 KD) at 24 h of heat stress and 48 of normal conditions. Proteomic analysis showed that 81 and 930 targets are involved in gene and protein expression respectively. At 4 h, 57 genes and 110 proteins in C4 reached 56 genes and 173 proteins in T4. At 24 h, 63 genes and 180 proteins in C24 decreased to 54 genes and 151 protein in T24. After 48 h, 56 genes and 136 proteins in C48 increased to 64 genes and 180 proteins in T48. The genes and proteins involved in transcription, translation, photosynthesis, transport, and other unknown metabolic processes, were differentially expressed under treatments of heat stress. These findings provide insights into the molecular mechanisms related to heat stress, in addition to its influence on the physiological traits of T. propinqua seedlings. Heat stress-mediated differential regulation genes indicate a role in the development and stress response of T. propinqua . The candidate dual-specificity genes and proteins identified in this study paves way for more molecular analysis of up-and-down-regulation.

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“…In both wheat and barley, the expression of Rubisco large subunit under water de cit condition decreased [53,54]. In the thylakoid membrane of rice, Rubisco plays a role in defense and regulation of photosynthesis under moderate heat stress [55]. In barley mutant, the defective PSI assembly causes photoinhibition and degradation of PSII due to a temperature-sensitive defect in ycf3 splicing [56].…”

Section: Discussionmentioning

confidence: 99%

Differential Expression Gene/Protein Contribute to Heat Stress-Responsive in Tetraena propinqua in Saudi Arabia

Alafari¹,

Abd-Elgawad

2021

Preprint

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BackgroundWithin their natural habitat, plants are subjected to abiotic stresses that include heat stress. In the current study, the effect of 4h, 24h and 48h of heat stress on Tetraena propinqua ssp. migahidii seedling’s protein profile and proteomic analyses were investigated. ResultsTotal soluble protein SDS-PAGE profile showed 18-protein bands downregulated at 4h and 48h, however, 20-protein bands were upregulated at 24h of heat stress. A proteomic analysis showed that 81 and 59 targets are involved in gene and protein expression respectively. ConclusionsThe genes and proteins involved in transcription, translation, photosynthesis, transport and other unknown metabolic processes, were differentially expressed under treatments of heat stress. These ﬁndings provide insights into the molecular mechanisms related to heat stress, in addition to its inﬂuence on the physiological traits of T. propinqua seedlings. Heat stress mediated differential regulation genes indicate a role in development and stress response of T. propinqua. The candidate dual specificity genes identified in this study paves way for more molecular analysis of up- and down-regulation.

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Leaf proteome pattern of two bread wheat varieties under water deficit stress conditions

Cited by 7 publications

References 61 publications

Proteomics approach to investigating osmotic stress effects on pistachio

Proteomics approach to investigating osmotic stress effects on pistachio

Differential expression gene/protein contribute to heat stress-responsive in Tetraena propinqua in Saudi Arabia

Differential Expression Gene/Protein Contribute to Heat Stress-Responsive in Tetraena propinqua in Saudi Arabia

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