Leaf glandular trichomes of micropropagated Inula britannica – Effect of sucrose on trichome density, distribution and chemical profile

Stanojković, Jelena N.; Todorović, Slađana; Pećinar, Ilinka; Lević, Steva; Ćalić, Snežana; Janošević, Dušica

doi:10.1016/j.indcrop.2020.113101

Cited by 7 publications

(3 citation statements)

References 58 publications

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“…This correlated well with a decreased amount of volatiles produced by the plants cultured in vitro [57]. Concerning culture medium composition, Stanojković et al [46] showed that the highest leaf trichome density was observed in Inula britannica plants grown in vitro on medium devoid of carbohydrates, and that the addition of sucrose to nutrient medium led to a decrease in the number of glandular trichomes per mm 2 on both leaf surfaces. Trichome density in all treatments was significantly higher on the abaxial side of I. britannica leaves.…”

Section: Effect Of Carbon Source On Glandular Trichome Characteristicsmentioning

confidence: 72%

“…The total number of C. pulegium glandular trichomes (P + C1 + C2) per leaf unit area on the adaxial leaf side did not vary with the treatment, but on the abaxial side the trichome density was significantly increased by glucose (433.3 ± 17.0 mm −2 ), compared to sucrose (335.9 ± 14.6 mm −2 ). Higher density of glandular trichomes on the abaxial relative to the adaxial leaf surface has been reported in a number of aromatic plant species [44][45][46]. Tozin et al [44] reported an increase in the density of glandular trichomes on the adaxial leaf surface of Ocimum gratissimum upon herbivory.…”

Section: Effect Of Carbon Source On Glandular Trichome Characteristicsmentioning

confidence: 91%

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Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source

Stojičić

Tošić

Stojanović

et al. 2022

Plants

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Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant–plant and plant–insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography–mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.

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Section: Effect Of Carbon Source On Glandular Trichome Characteristicsmentioning

confidence: 72%

Section: Effect Of Carbon Source On Glandular Trichome Characteristicsmentioning

confidence: 91%

Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source

Stojičić

Tošić

Stojanović

et al. 2022

Plants

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“…The dynamic genome-wide expression atlas and predicted interactome network indicates a transcriptional cross-talk between carbohydrate (sucrose and starch) metabolism in the leaf (source tissue) to the inulin biosynthesis in the root (sink tissue) as reported in related Inula species [ 45 ]. Additionally, unigenes encoding sucrose transport proteins (SUC8-like) with significant enrichment both in the leaf and stem indicates its involvement in metabolite translocation for the facilitation of inulin biosynthesis ( Figure 10 ) [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

An Integrative Transcriptional Network Revealed Spatial Molecular Interplay Underlying Alantolactone and Inulin Biosynthesis in Inula racemosa Hook f.

Seth

Devi

Sharma

et al. 2022

IJMS

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Inula racemosa Hook. f. (Pushkarmula), a perennial Himalayan herb known for its aromatic and phytopharmaceutical attributes, is not yet explored at genomic/transcriptomic scale. In this study, efforts were made to unveil the global transcriptional atlas underlying organ-specific specialized metabolite biosynthesis by integrating RNA-Seq analysis of 433 million sequenced reads with the phytochemical analysis of leaf, stem, and root tissues. Overall, 7242 of 83,772 assembled nonredundant unigenes were identified exhibiting spatial expression in leaf (3761), root (2748), and stem (733). Subsequently, integration of the predicted transcriptional interactome network of 2541 unigenes (71,841 edges) with gene ontology and KEGG pathway enrichment analysis revealed isoprenoid, terpenoid, diterpenoid, and gibberellin biosynthesis with antimicrobial activities in root tissue. Interestingly, the root-specific expression of germacrene-mediated alantolactone biosynthesis (GAS, GAO, G8H, IPP, DMAP, and KAO) and antimicrobial activities (BZR1, DEFL, LTP) well-supported with both quantitative expression profiling and phytochemical accumulation of alantolactones (726.08 μg/10 mg) and isoalantolactones (988.59 μg/10 mg), which suggests “roots” as the site of alantolactone biosynthesis. A significant interaction of leaf-specific carbohydrate metabolism with root-specific inulin biosynthesis indicates source (leaf) to sink (root) regulation of inulin. Our findings comprehensively demonstrate the source-sink transcriptional regulation of alantolactone and inulin biosynthesis, which can be further extended for upscaling the targeted specialized metabolites. Nevertheless, the genomic resource created in this study can also be utilized for development of genome-wide functionally relevant molecular markers to expedite the breeding strategies for genetic improvement of I. racemosa.

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Emergence of Phytochemical Genomics: Integration of Multi-Omics Approaches for Understanding Genomic Basis of Phytochemicals

Vijay

Kumar

Radhakrishnan

et al. 2022

Phytochemical Genomics

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Leaf glandular trichomes of micropropagated Inula britannica – Effect of sucrose on trichome density, distribution and chemical profile

Cited by 7 publications

References 58 publications

Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source

Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source

An Integrative Transcriptional Network Revealed Spatial Molecular Interplay Underlying Alantolactone and Inulin Biosynthesis in Inula racemosa Hook f.

Emergence of Phytochemical Genomics: Integration of Multi-Omics Approaches for Understanding Genomic Basis of Phytochemicals

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