Lead (Pb)-resistant bacteria inhibit Pb accumulation in dill (Anethum graveolens L.) by improving biochemical, physiological, and antioxidant enzyme response of plants

Rahbari, Akram; Fatemi, Hamideh; Pour, Behrooz Esmaiel; Rizwan, Muhammad; Soltani, Ahmad

doi:10.1007/s11356-020-10851-8

Cited by 19 publications

(7 citation statements)

References 64 publications

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“…These ndings are consistent with those of the previous research reporting the role of bacterial inoclation in conferring plant tolerance against heavy metals by boosting various antioxidative enzymes activity(Islam et al 2014;Shah et al 2020). This could be due to the production of metabolites by bacteria, including growth promoting hormones, which plays an important role in the synthesis of antioxidant enzymes(Rahbari et al 2021). In fact, SOD…”

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confidence: 99%

Plant growth promoting rhizobacteria modulates the antioxidant defense and the expression of stress-responsive genes providing Pb accumulation and tolerance of grass pea

Abdelkrim

Abid

Chaieb

et al. 2022

Environ Sci Pollut Res

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To ensure the success of phytoremediation, it is important to consider the appropriate combination of plants and microorganisms. This study was conducted to get a better insight into the underlying molecular and biochemical mechanism of grass pea (Lathyrus sativus L.) induced by plant growth promoting rhizobacteria (PGPR), when exposed for 3, 6, 9 and 14 days to 1 mM Pb in a hydroponic system.The signi cant positive effect of bacterial inoculation was reproduced in various parameters. Results indicated that inoculation of PGPR signi cantly increased the concentration of Pb by 20%, 66%, 43% and 36%, in roots and by 46%, 55%, 37%, 46% in shoots, respectively after 3, 6, 9 and 14 days of metal exposure as compared to the uninoculated plants. The metal accumulation in grass pea plants triggered a signi cant elevation in the synthesis of non-protein thiols (NPT), particularly in inoculated plant leaves where it was about 3 and 2-fold higher than the uninoculated set at 6 and 9 d. Nevertheless, Pb treatment signi cantly increased oxidative stress and membrane damage in leaves with the highest hydrogen peroxide (H 2 O 2 ) production and tissue malondialdehyde (MDA) concentration recorded in uninoculated plants. Further, the inoculation alleviated the oxidative stress, improved plant tolerance and modulated the activities of antioxidant enzymes (SOD, CAT, APX, GR, DHAR and MDHAR). Similary, the expression patterns of LsPCS, LsGCN, LsCNGC, LsGR and LsGST through qRT-PCR demonstrated that bacterial inoculation signi cantly induced gene expression levels in leaves 6 d after Pb treatment, indicating that PGPR act as regulators of stress responsive genes.The ndings suggest the key role of PGPR (R. leguminosarum (M5) + Pseudomonas uorescens (K23) + Luteibacter sp. + Variovorax sp.) in enhancing Pb accumulation, reducing metal toxicity, strengthening of the antioxidant system and conferring higher Pb tolerance to grass pea plants. Hence, the association Lathyrus sativus-PGPR is an effective tool to achieve the goal of remediation of Pb contaminated sites.

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confidence: 99%

Plant growth promoting rhizobacteria modulates the antioxidant defense and the expression of stress-responsive genes providing Pb accumulation and tolerance of grass pea

Abdelkrim

Abid

Chaieb

et al. 2022

Environ Sci Pollut Res

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“…P. Vijay and PM 25 counterbalance the increased ROS concentration developed due to Pb toxicity by regulating antioxidant enzymes' activity in a synchronized manner. Increased enzymatic actions under Pb stress in the current study for detoxification are all-embracing by many other earlier studies: It has been shown that metal-induced oxidative stress leads to ROS manifestation of ROS-activated enzymes(Rahbari et al 2020). Unlike phytochelatins and vacuolar compartmentalization of metals, which acts as the primary protection system in plants against metal toxicity, antioxidant enzymes are the second line of defense against metal-induced oxidative stress.…”

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confidence: 53%

“…In both P. Vijay and PM 25 cultivars, CAT enzyme activity amplifies with an increase in Pb and EDTA concentration, which indicates the efficient H2O2-scavenging system in the plants. The coordination in the enhancement of CAT and SOD parallel to the concentration of treatment plays a critical part in the scavenging procedure of O2and H2O2 (Khan et al 2019;Huang et al 2020;Huihui et al 2020;Rahbari et al 2020). However, the coordination between CAT and SOD amplification was more effective in P. Vijay than in PM 25.…”

Section: Discussionmentioning

confidence: 99%

Impact of ethylene diamine tetraacetic acid on physiochemical parameters and yield attribute in two varieties of Brassica juncea under lead stress

Naaz

Alam

Kumar

2023

Preprint

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Lead is one of the most toxic elements on earth. The harmful effects of lead at higher concentrations were seen on plant vegetation because plants are directly exposed to toxic levels. A previous work with B. juncea varieties were already screened against Pb to assess their endurance capacity from which B. juncea P. Vijay was opted out as the most tolerant, and B. juncea PM 25 was selected as the sensitive cultivar. Indian mustard, a well-known hyperaccumulator plant, is the most promising environmental crop used in diverse situations for ecological clean-up, and a chelating agent, EDTA, having remarkable efficacy, was used in the experiment. In the present study, the pot experiment was conducted in soil pretreated with 1000 mgkg− 1 Pb soil, and pots were augmented with different EDTA (2–10 mmol) concentrations. All the growth parameters were reduced more drastically in plants treated with Pb, however, a non-significant reduction was observed in 5 mmol EDTA treatments. Photosynthetic pigments, yield, nitrate reductase activity, NPK content were affected negatively, in contrast superoxide dismutase, catalase activity, and the ratio of chl a and chlb were increased in Pb and Pb + EDTA treated both varieties. All the parameters of PM 25 variety were affected more than P. Vijay during the experiment. Pb accumulation was elevated significantly by the augmentation of up to 5mmol EDTA in both cultivars. Accumulation of Pb in the shoot was higher in PM 25 than in P. Vijay, whereas root Pb accumulation shows the opposite, i.e., more Pb in roots of P. Vijay than PM 25. However, P. Vijay was found to be more effective in terms of Pb accumulation per plant than PM 25. Hence in the present work, we found that augmentation of Pb-polluted soil with EDTA works well while dealing with B. juncea assisted phytoremediation, and P. Vijay were found to be stronger candidate than PM 25. Also, we found that 5 mmol of EDTA was optimum for phytoremediation of the soil polluted with up to 1000 mg Pb kg− 1 soil.

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“…Many factors contribute to a strain’s potential to promote plant growth under heavy metal stress, including phosphate solubilization, IAA production, and nutrient availability [ 78 , 79 ]. In the current study, the strain LMR291 produced more auxin and ACC deaminase than the other strains tested, was able to solubilize phosphate, and tolerated three heavy metals (Pb, Zn, and As), which could explain why this strain, in particular, increased the biomass and photosynthetic activities of Sulla plants better compared to the other strains.…”

Section: Discussionmentioning

confidence: 99%

Native Heavy Metal-Tolerant Plant Growth Promoting Rhizobacteria Improves Sulla spinosissima (L.) Growth in Post-Mining Contaminated Soils

2022

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The potential of rhizobacteria in assisting plants used in the phytostabilization or re-vegetation of soils contaminated by heavy metals is gaining interest all around the world. In this context, six rhizobacterial strains isolated from highly heavy metal-contaminated soils situated in abandoned mining sites around the Oujda region (Morocco) were tested with Sulla spinosissima (L.), a native leguminous plant expanding in this area. The strains used were multi-resistant to heavy metals and possessed multiple plant growth-promoting traits. Potential beneficial effects of the strains were also evaluated in planta by measuring various growth and physiological parameters of inoculated Sulla plants grown in sterilized sand. Inoculation with the Rhodococcus qingshengii strain LMR340 boosted plant biomass (39% to 83% increase compared to uninoculated plants), chlorophyll and carotenoid content (up to 29%), and antioxidant enzyme activities (15% to 80% increase). Based on these interesting findings, selected strains were inoculated into plants growing in a heavy metal, multi-polluted, and poor soil. Under these conditions, non-inoculated plants and those inoculated with the strain LMR250 were unable to grow, while the other five bacterial inoculants restored plant growth. The best performing strain, Pseudarthrobacter oxydans LMR291, could be considered as a good biofertilizer and/or biostimulant candidate to be used for promoting the growth of selected plants in re-vegetation and/or phytostabilization programs of degraded and contaminated soils.

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Lead (Pb)-resistant bacteria inhibit Pb accumulation in dill (Anethum graveolens L.) by improving biochemical, physiological, and antioxidant enzyme response of plants

Cited by 19 publications

References 64 publications

Plant growth promoting rhizobacteria modulates the antioxidant defense and the expression of stress-responsive genes providing Pb accumulation and tolerance of grass pea

Plant growth promoting rhizobacteria modulates the antioxidant defense and the expression of stress-responsive genes providing Pb accumulation and tolerance of grass pea

Impact of ethylene diamine tetraacetic acid on physiochemical parameters and yield attribute in two varieties of Brassica juncea under lead stress

Native Heavy Metal-Tolerant Plant Growth Promoting Rhizobacteria Improves Sulla spinosissima (L.) Growth in Post-Mining Contaminated Soils

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