LEA13 and LEA30 Are Involved in Tolerance to Water Stress and Stomata Density in Arabidopsis thaliana

López-Cordova, Abigael; Ramírez-Medina, Humberto; Silva-Martínez, Guillermo A; González-Cruz, Leopoldo; Bernardino-Nicanor, Aurea; Huanca‐Mamani, Wilson; Montero-Tavera, Víctor; Tovar-Aguilar, Andrea; Ramírez-Pimentel, Juan Gabriel; Durán-Figueroa, N. V.; Acosta-García, Gerardo

doi:10.3390/plants10081694

Cited by 4 publications

(2 citation statements)

References 60 publications

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“…Furthermore, another factor influencing stomatal density is the level of certain protein groups in the late stages of embryogenesis (LEA). López-Cordova et al (2021) explained that LEA (Late Embryogenesis Abundant) protein is involved in the tolerance mechanism against drought stress and stomatal density. The result of this study found that both cowpea and long bean plants had a similar response by reducing the number of stomata (stomatal density) and the width of the stomatal opening under drought stress.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of morphophysiological responses among cowpea and long beans plants under drought stress condition

SYAFRIANI

SUSILO²,

SAYEKTI³

et al. 2022

Biodiversitas

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Abstract. Syafriani E, Susilo KR, Sayekti RS, Widyawan MH, Khoirunnisa NS, Rahmadhani M. 2022. Comparative study of morphophysiological responses among cowpea and long beans plants under drought stress condition. Biodiversitas 23: 5507-5518. Long bean plants are generally more susceptible to drought stress than cowpea plants. One of the promising solutions is through plant breeding to produce genetically modified long bean plants resistant to drought stress by crossing with cowpeas. Parental selection is the initial stage of the plant breeding program. This stage was performed by selecting parental varieties based on the desired superior characteristics, including morphological and physiological characteristics, as well as, yield production. Unfortunately, the comparison between long bean plants and cowpea plants is not well-studied. Therefore, the aim of this study was to analyze the morphophysiological similarities and differences between long beans and cowpeas to withstand drought stress. A two-factorial randomized block design was used in this study and consisted of 2 factors (plant varieties and type of drought stress). The plant varieties used in this study are 7 long bean plants and 7 cowpea plants. While the level of drought stress were: T0 (watering every day), T1 (every 5 days), and T2 (every 10 days). The observational data from morphophysiological characters indicated that long bean plants were more tolerant to drought stress than cowpea plants. Interestingly, one of the cowpea varieties (C2) had the highest proline contents (20.27 µmol/gram of leaves sample) compared to other varieties. Hence, the C2 variety can be considered a candidate for further studies. Meanwhile, all the tested 7 varieties of long bean plants showed varying adaptability, possibly due to the genetic diversity of each variety against drought stress.

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Section: Discussionmentioning

confidence: 99%

Comparative study of morphophysiological responses among cowpea and long beans plants under drought stress condition

SYAFRIANI

SUSILO²,

SAYEKTI³

et al. 2022

Biodiversitas

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“…Late embryogenesis abundant (LEA) proteins are a large protein family that mainly functions in protecting cells from abiotic stress; however, these proteins are also involved in drought tolerance and the determination of stomatal patterning and density. The overexpression of LEA13 and LEA30 , CaLEA6 and CaLEA73 (from Capsicum annuum ), and MsLEA4-4 (from Medicago sativa ) genes in Arabidopsis thaliana plants increased drought tolerance due to lower transpiration and stomatal density compared to control plants [ 82 ]. Homeodomain leucine zipper (HD-Zip) transcription factors are plant-specific TFs that participate in abiotic stress responses, and MdHB7-like (an HD-Zip I TF) was identified as a drought-induced gene in apple plants.…”

Section: Molecular Regulation Of Stomatal Density For Osmotic Stress ...mentioning

confidence: 99%

How Does Stomatal Density and Residual Transpiration Contribute to Osmotic Stress Tolerance?

Hasanuzzaman

Zhou

Shabala

2023

Plants

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Osmotic stress that is induced by salinity and drought affects plant growth and development, resulting in significant losses to global crop production. Consequently, there is a strong need to develop stress-tolerant crops with a higher water use efficiency through breeding programs. Water use efficiency could be improved by decreasing stomatal transpiration without causing a reduction in CO2 uptake under osmotic stress conditions. The genetic manipulation of stomatal density could be one of the most promising strategies for breeders to achieve this goal. On the other hand, a substantial amount of water loss occurs across the cuticle without any contribution to carbon gain when the stomata are closed and under osmotic stress. The minimization of cuticular (otherwise known as residual) transpiration also determines the fitness and survival capacity of the plant under the conditions of a water deficit. The deposition of cuticular wax on the leaf epidermis acts as a limiting barrier for residual transpiration. However, the causal relationship between the frequency of stomatal density and plant osmotic stress tolerance and the link between residual transpiration and cuticular wax is not always straightforward, with controversial reports available in the literature. In this review, we focus on these controversies and explore the potential physiological and molecular aspects of controlling stomatal and residual transpiration water loss for improving water use efficiency under osmotic stress conditions via a comparative analysis of the performance of domesticated crops and their wild relatives.

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Histone Deacetylases HD2A and HD2B Undergo Feedback Regulation by ABA and Modulate Drought Tolerance via Mediating ABA-Induced Transcriptional Repression

Han,

Haouel,

Georgii

et al. 2023

Genes

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Histone deacetylation catalyzed by histone deacetylase plays a critical role in gene silencing and subsequently controls many important biological processes. It was reported that the expression of the plant-specific histone deacetylase subfamily HD2s is repressed by ABA in Arabidopsis. However, little is known about the molecular relationship between HD2A/HD2B and ABA during the vegetative phase. Here, we describe that the hd2ahd2b mutant shows hypersensitivity to exogenous ABA during the germination and post-germination period. Additionally, transcriptome analyses revealed that the transcription of ABA-responsive genes was reprogrammed and the global H4K5ac level is specifically up-regulated in hd2ahd2b plants. ChIP-Seq and ChIP-qPCR results further verified that both HD2A and HD2B could directly and specifically bind to certain ABA-responsive genes. As a consequence, Arabidopsis hd2ahd2b plants displayed enhanced drought resistance in comparison to WT, which is consistent with increased ROS content, reduced stomatal aperture, and up-regulated drought-resistance-related genes. Moreover, HD2A and HD2B repressed ABA biosynthesis via the deacetylation of H4K5ac at NCED9. Taken together, our results indicate that HD2A and HD2B partly function through ABA signaling and act as negative regulators during the drought resistance response via the regulation of ABA biosynthesis and response genes.

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LEA13 and LEA30 Are Involved in Tolerance to Water Stress and Stomata Density in Arabidopsis thaliana

Cited by 4 publications

References 60 publications

Comparative study of morphophysiological responses among cowpea and long beans plants under drought stress condition

Comparative study of morphophysiological responses among cowpea and long beans plants under drought stress condition

How Does Stomatal Density and Residual Transpiration Contribute to Osmotic Stress Tolerance?

Histone Deacetylases HD2A and HD2B Undergo Feedback Regulation by ABA and Modulate Drought Tolerance via Mediating ABA-Induced Transcriptional Repression

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