Le nez au chevet du cerveau

Nivet, Emmanuel; Devèze, Arnaud; Girard, Stéphane; Roman, François S.; Féron, François

doi:10.1051/medsci/20112711006

Cited by 2 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They are multipotent, providing a potential source of stem cells for treating numerous types of tissue damages [ 13 , 14 ]. Moreover, human OE-MSCs can be quickly and easily propagated in sufficient numbers to meet the requirements for cell transplantation without showing tumorigenicity risks [ 14 , 24 – 26 ]. Although the therapeutic potential of OE-MSCs has not yet been assessed in human or veterinary medicine, their therapeutic effect has been evaluated in various rodent models of tissue damage such as myocardial infarct [ 27 ], spinal cord trauma [ 28 – 30 ], cochlear damage [ 31 ], Parkinson’s disease [ 32 ], and ischemic/hypoxic injury of the hippocampus [ 25 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera

et al. 2018

Self Cite

View full text Add to dashboard Cite

BackgroundStem cell-based therapies are an attractive option to promote regeneration and repair defective tissues and organs. Thanks to their multipotency, high proliferation rate and the lack of major ethical limitations, “olfactory ecto-mesenchymal stem cells” (OE-MSCs) have been described as a promising candidate to treat a variety of damaged tissues. Easily accessible in the nasal cavity of most mammals, these cells are highly suitable for autologous cell-based therapies and do not face issues associated with other stem cells. However, their clinical use in humans and animals is limited due to a lack of preclinical studies on autologous transplantation and because no well-established methods currently exist to cultivate these cells. Here we evaluated the feasibility of collecting, purifying and amplifying OE-MSCs from different mammalian genera with the goal of promoting their interest in veterinary regenerative medicine.Biopsies of olfactory mucosa from eight mammalian genera (mouse, rat, rabbit, sheep, dog, horse, gray mouse lemur and macaque) were collected, using techniques derived from those previously used in humans and rats. The possibility of amplifying these cells and their stemness features and differentiation capability were then evaluated.ResultsBiopsies were successfully performed on olfactory mucosa without requiring the sacrifice of the donor animal, except mice. Cell populations were rapidly generated from olfactory mucosa explants. These cells displayed similar key features of their human counterparts: a fibroblastic morphology, a robust expression of nestin, an ability to form spheres and similar expression of surface markers (CD44, CD73). Moreover, most of them also exhibited high proliferation rates and clonogenicity with genus-specific properties. Finally, OE-MSCs also showed the ability to differentiate into mesodermal lineages.ConclusionsThis article describes for the first time how millions of OE-MSCs can be quickly and easily obtained from different mammalian genera through protocols that are well-suited for autologous transplantations. Moreover, their multipotency makes them relevant to evaluate therapeutic application in a wide variety of tissue injury models. This study paves the way for the development of new fundamental and clinical studies based on OE-MSCs transplantation and suggests their interest in veterinary medicine.

show abstract

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…2), sans restriction d'âge ou de condition. L'isolement de la lamina propria et l'extraction des cellules souches olfactives (CSO) ont été réalisés avec succès par notre équipe chez 8 mammifères et chez l'homme [17].Leur potentiel thérapeutique a été testé avec succès dans différents modèles animaux de paraplégie, de la maladie de Parkinson, de surdité et d'amnésie [18]. Les mécanismes d'action suivants ont été observés :…”

Section: Les Cellules Souches Olfactives Nasalesunclassified

Utilisation des cellules souches olfactives dans la régénération des nerfs périphériques

Jaloux

Witters

Véran

et al. 2021

Bulletin de l'Académie Nationale de Médecine

View full text Add to dashboard Cite

Le nez au chevet du cerveau

Cited by 2 publications

References 14 publications

Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera

Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera

Utilisation des cellules souches olfactives dans la régénération des nerfs périphériques

Contact Info

Product

Resources

About