LC-MS-based metabolomics analysis of Berberine treatment in ulcerative colitis rats

Liao, Ziqiong; Zhang, Shaobao; Li, Wen; Zou, Baorong; Lei, Lin; Chen, Mingyi; Liu, Deliang; Wang, Mengxia; Li, Lin; Cai, Ying; Liao, Qiongfeng; Xie, Zefeng

doi:10.1016/j.jchromb.2019.121848

Cited by 34 publications

(24 citation statements)

References 36 publications

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“…Lipids are important substances for energy storage and energy supply in vivo, and excessively oxidized lipids in the intestine cause intestinal epithelial tissue damage [40,41]. Studies have found that berberine inhibits the expression of metabolites related to linoleic acid metabolism and sheath lipid metabolism in colitis, possibly due to alterations in the metabolism of intestinal microorganisms adjusting their metabolism and reductions in the oxidation reaction in the intestine and protecting the intestinal epithelium [42].…”

Section: Discussionmentioning

confidence: 99%

Polyphenol Rich Forsythia suspensa Extract Alleviates DSS-Induced Ulcerative Colitis in Mice through the Nrf2-NLRP3 Pathway

et al. 2022

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This study systematically evaluated the effect of Forsythia suspensa extract on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) and determined its mechanism of action. The results showed that Forsythia suspensa extract significantly inhibited DSS-induced UC in mice. In vivo mechanistic studies revealed that Forsythia suspensa extract relieved the symptoms of colitis by enhancing antioxidant activity and inhibiting pyroptosis. Further in vitro experiments on the mechanism of Forsythia suspensa showed that it reduced the level of reactive oxygen species (ROS) in J774A.1 cells. We found that Forsythia suspensa extract enhanced cellular antioxidation activity and inhibited pyroptosis. After silencing NLRP3, it was found to play an important role in pyroptosis. In addition, after Nrf2 was silenced, the inhibitory effect of Forsythia suspensa extract on cell pyroptosis was eliminated, indicating an interaction between Nrf2 and NLRP3. Metabonomics revealed that Forsythia suspensa extract significantly improved metabolic function in colitis mice by reversing the abnormal changes in the levels of 9 metabolites. The main metabolic pathways involved were glutathione metabolism, aminoacyl-tRNA biosynthesis and linoleic acid metabolism. In conclusion, we found that Forsythia suspensa extract significantly alleviated DSS-induced UC injury through the Nrf2-NLRP3 pathway and relieved metabolic dysfunction.

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Section: Discussionmentioning

confidence: 99%

Polyphenol Rich Forsythia suspensa Extract Alleviates DSS-Induced Ulcerative Colitis in Mice through the Nrf2-NLRP3 Pathway

et al. 2022

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“…Purine metabolism is associated with amino acid metabolism through the purine nucleotide cycle and plays an important role in energy supply, metabolic regulation, and coenzyme production [ 41 ]. Purine metabolism includes the de novo purine synthesis pathway, salvage pathway, and degradation pathway [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolic Profile Reveals the Immunosuppressive Mechanisms of Methionyl-Methionine in Lipopolysaccharide-Induced Inflammation in Bovine Mammary Epithelial Cell

Lan

Ren

Wang

et al. 2021

Animals

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Our previous transcriptomic study found that methionyl-methionine (Met-Met) exerts an anti-inflammatory effect in the bovine mammary epithelial cell (MAC-T) at a molecular level. However, evidence of whether the metabolic production of Met-Met confers protection was scarce. To investigate the inflammatory response and metabolite changes of Met-Met in lipopolysaccharide (LPS)-induced inflammation of MAC-T, mass spectrometry-based metabolomics and qPCR were conducted. The increased levels of IL-8, TNF-α, AP-1, and MCP-1 were reduced by pretreating with 2 mM Met-Met after LPS exposure. Metabolomics profiling analysis demonstrated that LPS induced significant alteration of metabolites, including decreased tryptophan, phenylalanine, and histidine levels and increased palmitic acid and stearic acid levels as well as purine metabolism disorder, whereas Met-Met reversed these changes significantly. Pathways analysis revealed that overlapping metabolites were mainly enriched in the cysteine and methionine metabolism, fatty acids biosynthesis, and purines degradation. Correlation networks showed that the metabolic profile was significantly altered under the conditions of inflammation and Met-Met treatment. Collectively, Met-Met might relieve MAC-T cell inflammation via hydrolysate methionine, which further changes the processes of amino acid, purine, and fatty acid metabolism.

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“…Peroxisome inhibits the activity of NF-κB through negative regulation of transcription factors to achieve anti-in ammatory effects (Cao H et al,2018). The ulcerative colitis (UC) model shows that berberine can change the levels of various fecal metabolites related to various pathways such as the citrate cycle (Liao Z et al,2019). Valine can target colonic epithelial cells, high-a nity peptide transporter 1 (PepT1), which exhibits anti-in ammatory properties (Wu Y et al,2019).…”

Section: Discussionmentioning

confidence: 99%

Identification of molecular subgroups of ulcerative colitis by gene expression profile

Zhang

Feng

et al. 2021

Preprint

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Ulcerative colitis (UC) is a chronic inflammatory disease that is prone to recurrent attacks. It has complex pathogenesis, which is closely related to genetics, constitution, dietary habits, and environmental factors. From the comprehensive Gene Expression Omnibus database (GEO), we retrieved gene expression profiles and classified 197 cases of UC into three subgroups for the purpose of predicting the basic molecular characteristics for different types of ulcerative colitis. As expected, each group showed its own clinical peculiarity and way of presentation. In this article, consensus clustering was used to divide the sample into three. The WGCNA analysis was applied to evaluate specific modules and reveal transcriptional differences among the subgroups. Subsequently, pathway and function enrichment analysis was conducted based on WGCNA. In subgroup Ⅰ, fructose and mannose metabolism pathway and cell cycle 11/36 pathway are up-regulated, which could be an indicator of exacerbation. Furthermore, the hematopoietic cell lineage pathway, which was significantly up-regulated in subgroup Ⅱ, might be indicating a disease correlation. In subgroup Ⅲ, the gene expression pattern of the peroxisome pathway is similar to the normal group, which may indicate an early stage of UC. Although no significant prognostic difference existing among the groups, there were significant differences in their underlying biological characteristics. This suggests that transcriptome classifications also represent risk factors for different disease states and ages. In summary, the bioinformatics techniques used in this study contribute to identifying molecular subtypes for diagnosing human ulcerative colitis. The transcriptome classification of UC cases suggests that each subgroup may have its own gene expression pattern and pathway, providing further personalized treatment guidance for patients with ulcerative colitis.

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LC-MS-based metabolomics analysis of Berberine treatment in ulcerative colitis rats

Cited by 34 publications

References 36 publications

Polyphenol Rich Forsythia suspensa Extract Alleviates DSS-Induced Ulcerative Colitis in Mice through the Nrf2-NLRP3 Pathway

Polyphenol Rich Forsythia suspensa Extract Alleviates DSS-Induced Ulcerative Colitis in Mice through the Nrf2-NLRP3 Pathway

Metabolic Profile Reveals the Immunosuppressive Mechanisms of Methionyl-Methionine in Lipopolysaccharide-Induced Inflammation in Bovine Mammary Epithelial Cell

Identification of molecular subgroups of ulcerative colitis by gene expression profile

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