Layer-by-layer printing of cells and its application to tissue engineering

Kesari, Priya; Xu, Tao; Boland, Thomas

doi:10.1557/proc-845-aa4.5

Cited by 24 publications

(23 citation statements)

References 20 publications

(16 reference statements)

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“…Some examples of chemical functionalization include: methacrylation and acetylation of gelatin (modifies degradation) [54,56], oxidation of alginate (modifies degradation) [42,64], and synthesis of a block co-polymer comprised of poly(N-(2-hydroxypropyl)methacrylamide lactate) [p(HPMAm-lactate)] and PEG (improves biodegradability) [62]. Some examples of blends include fibrin and alginate (improves biological activity) [22,54,61,65,66], alginate and gelatin, alginate and gelatin in modified and unmodified forms [55], alginate, gelatin and hydroxyapatite (optimized for bone tissue engineering) [58], thermoresponsive poly(N-isopropylacrylamide) grafted hyaluronan (HA-pNIPAAM) blended with methacrylated hyaluronan (HAMA) (to improve printability) [63], gelatin with hyaluronic acid or gellan gum (to improve cell behavior towards bone tissue engineering, mechanical properties and printability) [55,59,60,67]. However, even with these modifications, there is an urgent need for further development of bioinks to improve the mechanical properties, gelation process, cytocompatibility, degradation rate, tissue specificity, and adaptability to clinical set-ups.…”

Section: Established Bioinksmentioning

confidence: 99%

Biofabrication of 3D constructs: fabrication technologies and spider silk proteins as bioinks

DeSimone

Schacht

Jungst

et al. 2015

Pure and Applied Chemistry

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Despite significant investment in tissue engineering over the past 20 years, few tissue engineered products have made it to market. One of the reasons is the poor control over the 3D arrangement of the scaffold's components. Biofabrication is a new field of research that exploits 3D printing technologies with high spatial resolution for the simultaneous processing of cells and biomaterials into 3D constructs suitable for tissue engineering. Cell-encapsulating biomaterials used in 3D bioprinting are referred to as bioinks. This review consists of: (1) an introduction of biofabrication, (2) an introduction of 3D bioprinting, (3) the requirements of bioinks, (4) existing bioinks, and (5) a specific example of a recombinant spider silk bioink. The recombinant spider silk bioink will be used as an example because its unmodified hydrogel format fits the basic requirements of bioinks: to be printable and at the same time cytocompatible. The bioink exhibited both cytocompatible (selfassembly, high cell viability) and printable (injectable, shear-thinning, high shape fidelity) qualities. Although improvements can be made, it is clear from this system that, with the appropriate bioink, many of the existing faults in tissue-like structures produced by 3D bioprinting can be minimized.

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Section: Established Bioinksmentioning

confidence: 99%

Biofabrication of 3D constructs: fabrication technologies and spider silk proteins as bioinks

DeSimone

Schacht

Jungst

et al. 2015

Pure and Applied Chemistry

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“…[53] In these multicellular aggregates, the need for supporting gels or matrices is eliminated, the adverse effects 3D culture approach for generating a laminated cerebral cortex like structure from pluripotent stem cells. [57,58] Microfabrication Neuroprogenitor cells Microfluidic culture platform containing a relief pattern of soma and axonal compartments connected by microgrooves to direct, isolate, lesion, and biochemically analyze CNS axons [67,68] 3D bioprinting Primary human cortical neurons Discrete layers of primary neutrons in a RGD peptide-modified gellan gum [118][119][120] Intestine (Gut) Self-assembled Stem cells Identified intestinal stem cells and differentiated cells in vitro [59,60] Microfabrication Human epithelial cells Mimic contractility by using mechanochemical actuator [11,19,27,72] Liver Self-assembled Human stem cells 3D culture of self-renewing human liver tissue [61,62] Microfabrication Hepatocytes and fibroblasts Microengineered hepatic microtissues containing hepatocytes and fibroblasts [73][74][75][76][77] 3D bioprinting HepG2 and HUVEC Multilayered organ tissue model [96,[155][156][157] Vessel Microfabrication Rat brain endothelial cells 3D culture in microfluidic device [63][64][65][66] 3D bioprinting HUVECs and HUVSMCs Scaffold-less vessel formation using spheroid fusion [84][85][86][87][88][89][90][91]…”

Section: Engineering Technologiesmentioning

confidence: 99%

“…The fabrication of tubular hydrogel structures by drop-on-demand inkjet printing was first attempted by Kesari and co-workers in 2005. [84] Next, Nakamura et al pioneered a direct, alginate droplet based, bioprinting technique to pattern 3D vessels. [85,86] They formed fibers and tubes by the addition of alginate droplets to a calcium chloride (CaCl 2 ) solution.…”

Section: Bioprinted Vesselsmentioning

confidence: 99%

3D Miniaturization of Human Organs for Drug Discovery

Park

Wetzel

Dréau

2017

Adv. Healthcare Mater.

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“Engineered human organs” hold promises for predicting the effectiveness and accuracy of drug responses while reducing cost, time, and failure rates in clinical trials. Multiorgan human models utilize many aspects of currently available technologies including self‐organized spherical 3D human organoids, microfabricated 3D human organ chips, and 3D bioprinted human organ constructs to mimic key structural and functional properties of human organs. They enable precise control of multicellular activities, extracellular matrix (ECM) compositions, spatial distributions of cells, architectural organizations of ECM, and environmental cues. Thus, engineered human organs can provide the microstructures and biological functions of target organs and advantageously substitute multiscaled drug‐testing platforms including the current in vitro molecular assays, cell platforms, and in vivo models. This review provides an overview of advanced innovative designs based on the three main technologies used for organ construction leading to single and multiorgan systems useable for drug development. Current technological challenges and future perspectives are also discussed.

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“…Tissue printing is an attractive scaffold free technique with the great potential of constructing delicate 3D tissue-like structures. [51][52][53][54][55][56][57][58][59][60][61] (Fig. 6A-F).…”

Section: Directed Assembly Of Cell-laden Hydrogels For Engineering Timentioning

confidence: 99%