Latilactobacillus curvatus: A Candidate Probiotic with Excellent Fermentation Properties and Health Benefits

Abstract

Latilactobacillus curvatus is a candidate probiotic that has been included in the list of recommended biological agents for certification by the European Food Safety Authority. According to the published genomic information, L. curvatus has several genes that encode metabolic pathways of carbohydrate utilization. In addition, there are some differences in cell surface complex related genes of L. curvatus from different sources. L. curvatus also has several genes that encode bacteriocin production, which can produce Curvacin A and Sakacin P. Due to its ability to produce bacteriocin, it is often used as a bioprotective agent in fermented meat products, to inhibit the growth of a variety of pathogenic and spoilage bacteria. L. curvatus exerts some probiotic effects, such as mediating the production of IL-10 by dendritic cells through NF-κB and extracellular regulated protein kinases (ERK) signals to relieve colitis in mice. This review is the first summary of the genomic and biological characteristics of L. curvatus. Our knowledge on its role in the food industry and human health is also discussed, with the aim of providing a theoretical basis for the development of applications of L. curvatus.

Isolation, identification and characterization of Lactobacillus species diversity from Meekiri: traditional fermented buffalo milk gels in Sri Lanka

“…L. fermentum (Type-III), L. curvatus and L. acidophilus showed the highest (p < 0.05) pH reduction in both cow's and buffalo's milk. In L. curvatus species several genes associated with metabolic pathways encoding proteins that are involved with carbohydrate utilization were identified (Chen et al, 2020) and it may result in high production of acids in milk leading to a high pH reduction as seen in the present study. In both situations, L. fermentum (Type-I), showed the lowest (p < 0.05) pH reduction and it may be associated with its facultative heterofermentative nature which uses substrates to produce both acid and non-acidic substances depending on the situation.…”

Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria

“…The literature data showed that different isolates of Lb. curvatus exhibit beneficial role in dyslipidemia and obesity 26 . Moreover, in the case of Lb.…”

Screening and Identification of Latilactobacillus curvatus Z12 From Rumen Fluid of an Adult Female Sika Deer as a Potential Probiotic for Feed Additives

“…In this study, Z12 had no lytic activity against sheep blood erythrocytes, so it can be considered a safe candidate for probiotics. Generally, LAB are sensitive to broad-spectrum antibiotics (e.g., tetracycline and chloramphenicol), macrolides (azithromycin), glycopeptides (vancomycin), and β-lactam antibiotics (e.g., ampicillin, penicillin, and cephalothin) but resistant to aminoglycosides (e.g., streptomycin and gentamicin) ( 25 , 32 ). However, in our study, some contradictory resistance results were found for Z12 , which displayed resistance to ampicillin, vancomycin, tetracycline, and chloramphenicol but sensitivity to penicillin G and cefamandole and intermediate resistance to azithromycin.…”

“…In fact, there are various opinions as to whether probiotics showing resistance to specific antimicrobials are desirable since antimicrobial-resistant probiotics also have potentially negative effects, such as the transfer of resistance to intestinal pathogens or antibiotic-induced diarrhea ( 25 ). However, previous studies have shown that mobile elements such as phage, transposases and insertion sequences were not found in the complete genomes of L. curvatus SRCM103465 (CP035110.1), L. curvatus MARS6 (CP022474), and L. curvatus 20,019 (CP026116.1), as well as their upstream and downstream 5 kbp sequences downloaded from NCBI, indicating a low transfer risk of antimicrobial resistance genes in L. curvatus species ( 32 ).…”

“…Since antimicrobial resistance is studied by using breakpoint values to classify microorganisms as resistant or susceptible, neither EUCAST nor CLSI guidelines have been defined for the breakpoints of the disc-diffusion method for studying antibiotic resistance of LAB ( 30 , 31 ). Therefore, the breakpoints were defined and classified for each antimicrobial based on the CLSI guidelines ( Table 1 ), and the results were compared with the existing literature to verify the classification of resistance ( 18 , 32 ). Separate experiments were performed in triplicate.…”