Latex protein extracts from Calotropis procera with immunomodulatory properties protect against experimental infections with Listeria monocytogenes

Nascimento, Danielle Cristina de Oliveira; Ralph, Maria Taciana; Batista, Jacqueline Ellen Camelo; Silva, Diogo Manoel Farias; Gomes-Filho, M.A.; Alencar, Nylane Maria Nunes de; Leal, Nilma Cintra; Ramos, Márcio V.; Lima‐Filho, José Vitor

doi:10.1016/j.phymed.2016.03.012

Cited by 16 publications

(3 citation statements)

References 28 publications

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“…The first report suggesting the participation of the latex proteins in pharmacological benefits has described anti-inflammation in three different animal models ( Alencar, 2004 ). Since then, the latex proteins have been implicated in modulation of different inflammatory processes of clinical relevance, such as severe bacterial infections ( Nascimento, 2016 ) and colitis ( Kumar et al, 2019 ), as well as in ameliorating side effects of antimetabolic drugs prescribed in cancer therapy ( Alencar et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Phytomodulatory proteins isolated from Calotropis procera latex promote glycemic control by improving hepatic mitochondrial function in HepG2 cells

Oliveira

Araújo²,

Lima³

et al. 2021

Saudi Pharmaceutical Journal

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The medicinal uses of Calotropis procera are diverse, yet some of them are based on effects that still lack scientific support. Control of diabetes is one of them. Recently, latex proteins from C. procera latex (LP) have been shown to promote in vivo glycemic control by the inhibition of hepatic glucose production via AMP-activated protein kinase (AMPK). Glycemic control has been attributed to an isolated fraction of LP (CpPII), which is composed of cysteine peptidases (95%) and osmotin (5%) isoforms. Those proteins are extensively characterized in terms of chemistry, biochemistry and structural aspects. Furthermore, we evaluated some aspects of the mitochondrial function and cellular mechanisms involved in CpPII activity. The effect of CpPII on glycemic control was evaluated in fasting mice by glycemic curve and glucose and pyruvate tolerance tests. HepG2 cells was treated with CpPII, and cell viability, oxygen consumption, PPAR activity, production of lactate and reactive oxygen species, mitochondrial density and protein and gene expression were analyzed. CpPII reduced fasting glycemia, improved glucose tolerance and inhibited hepatic glucose production in control animals. Additionally, CpPII increased the consumption of ATP-linked oxygen and mitochondrial uncoupling, reduced lactate concentration, increased protein expression of mitochondrial complexes I, III and V, and activity of peroxisome-proliferator-responsive elements (PPRE), reduced the presence of reactive oxygen species (ROS) and increased mitochondrial density in HepG2 cells by activation of AMPK/PPAR. Our findings strongly support the medicinal use of the plant and suggest that CpPII is a potential therapy for prevention and/or treatment of type-2 diabetes. A common epitope sequence shared among the proteases and osmotin is possibly the responsible for the beneficial effects of CpPII.

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Section: Discussionmentioning

confidence: 99%

Phytomodulatory proteins isolated from Calotropis procera latex promote glycemic control by improving hepatic mitochondrial function in HepG2 cells

Oliveira

Araújo²,

Lima³

et al. 2021

Saudi Pharmaceutical Journal

Self Cite

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“…It also prompted TNF-α and IL-6 mRNA transcripts after infection which resulted in higher efficacy of intracellular bacterial killing. Additional in vivo studies on Swiss mice have shown that even one administration of C. procera latex resulted in higher survival rate, ease of symptoms of infection, and accumulation of leukocytes in bloodstream along with its increased migration into the peritoneal cavity [154].…”

Section: Immunomodulatory Propertiesmentioning

confidence: 99%

Functional Studies of Plant Latex as a Rich Source of Bioactive Compounds: Focus on Proteins and Alkaloids

Gracz-Bernaciak

Mazur

Nawrot

2021

IJMS

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Latex, a sticky emulsion produced by specialized cells called laticifers, is a crucial part of a plant’s defense system against herbivory and pathogens. It consists of a broad spectrum of active compounds, which are beneficial not only for plants, but for human health as well, enough to mention the use of morphine or codeine from poppy latex. Here, we reviewed latex’s general role in plant physiology and the significance of particular compounds (alkaloids and proteins) to its defense system with the example of Chelidonium majus L. from the poppy family. We further attempt to present latex chemicals used so far in medicine and then focus on functional studies of proteins and other compounds with potential pharmacological activities using modern techniques such as CRISPR/Cas9 gene editing. Despite the centuries-old tradition of using latex-bearing plants in therapies, there are still a lot of promising molecules waiting to be explored.

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“…Swiss mice (n = 4) were euthanized, and the peritoneal cavity was injected with 5-10 ml of Roswell Park Memorial Institute medium (RPMI; Sigma-Aldrich/Merck, USA) supplemented with 100 u/ml of penicillin and 100 μg/ml of streptomycin (Nascimento et al, 2016).…”

Section: Peritoneal Macrophage Cell Culturesmentioning

confidence: 99%

Antiinflammatory and antiinfective effect of caffeine in a mouse model of disseminated salmonellosis

Dorvigny

Tavares

Almeida

et al. 2021

Phytotherapy Research

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Caffeine has been reported for its antiinflammatory properties by stimulating phagocytosis. In this study, we investigated the antiinflammatory and antiinfective potential of caffeine in murine macrophage cell cultures and Swiss mice infected with virulent Salmonella enterica serotype typhimurium. Peritoneal macrophages (pMØ) were treated with caffeine on 96-well plates for 24 hr and then infected with Salmonella for 4 hr. In another experiment, the pMØ were first infected with the bacterium for 4 hr and then treated with caffeine for 24 hr. In addition, Swiss mice were inoculated, intraperitoneally, with S. typhimurium and then received caffeine intravenously. Control groups received phosphate-buffered saline (PBS) or dexamethasone. We found that treatments with caffeine increased the macrophage cell viability and reduced the intracellular bacterial load. The administration of caffeine to Swiss mice reduced the infiltration of leukocytes into the peritoneal cavity after the bacterial challenge.Furthermore, the bacterial burdens in the peritoneal fluid, bloodstream, spleen, and liver were decreased by caffeine treatment. The expression levels of tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), IL-6, and inducible nitric oxide synthase (iNOs) were down-regulated after infection in caffeine-treated mice. We can conclude that caffeine has both antiinflammatory and antiinfective properties that can be useful for management of bacterial infections along with antibiotics.

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Latex protein extracts from Calotropis procera with immunomodulatory properties protect against experimental infections with Listeria monocytogenes

Cited by 16 publications

References 28 publications

Phytomodulatory proteins isolated from Calotropis procera latex promote glycemic control by improving hepatic mitochondrial function in HepG2 cells

Phytomodulatory proteins isolated from Calotropis procera latex promote glycemic control by improving hepatic mitochondrial function in HepG2 cells

Functional Studies of Plant Latex as a Rich Source of Bioactive Compounds: Focus on Proteins and Alkaloids

Antiinflammatory and antiinfective effect of caffeine in a mouse model of disseminated salmonellosis

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