1995
DOI: 10.1021/bi00036a033 View full text |Buy / Rent full text
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Abstract: The lateral organization of fluid cholesterol-dimyristoylphosphatidylcholine (DMPC) bilayers was studied by measuring the response of fluorescent membrane probes, dipyrenylphosphatidylcholines (diPyrxPCs) or merocyanine 540, to the variation of cholesterol concentration. Parallel absorbance and light-scattering measurements were also carried out. The excimer-to-monomer ratio of diPyrxPCs displayed abrupt deviations at particular cholesterol mole fractions (CMFs). The most notable of these occurred at CMFs of 0… Show more

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“…As seen in . Because the cholesterol concentration increments were moderate (5 mol%), the data were smooth instead of punctuated as observed when tiny increments are used in the vicinity of concentration that generate cholesterol superlattices (25)(26)(27). A: MC540 emission spectra in liposomes composed of 100% sphingomyelin at 24jC (solid curve) and at 48jC (dashed curve).…”
Section: Mc540 Emission Spectramentioning
“…Theoretical analyses of the actions of cholesterol have revealed a variety of both general and specific effects, depending on the membrane composition (e.g., see Refs. 25,36,37). At concentrations above 20 mol% to 25 mol%, it induces formation of an Fig.…”
Section: Liposomesmentioning
See 1 more Smart Citation
“…Lipid bilayers containing cholesterol may also exhibit phase separation into cholesterol-rich (liquid-ordered) and cholesterol-poor (liquid disordered) domains with the increase of cholesterol content [11,[19][20][21][22][23][24], although the molecular organization of such cholesterol-containing bilayers is still a matter of debate, as is the significance of the structures like lipid rafts and hexagonal superlattices [25][26][27][28][29]. With the increasing fraction of cholesterol, the BLM became more stable at the beginning, and the threshold voltage of bilayer breakdown (rupture) was initially increased.…”
Section: Accepted M Manuscriptmentioning
“…In addition, from this composition (i.e., X er = 0.150.20), the stoichiometry of the molecular cluster is presumed to be 1:6 (ergosterol:DPPC), which is equivalent to X er = 0.143, by applying the superlattice view. 7,8 This view is a concept proposed to explain the tendency for sterol molecules to be distributed regularly within the binary bilayer and provides molecular pictures based on a hexagonal lattice for various types of possible regular distributions. We noticed that there is a slight difference between the presumed stoichiometry and the experimental threshold-like composition.…”
Section: ¹1mentioning