Lateral-flow technology: From visual to instrumental

Mak, Wing Cheung; Beni, Valerio; Turner, Anthony P. F.

doi:10.1016/j.trac.2015.10.017

Cited by 217 publications

(148 citation statements)

References 91 publications

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“…Lateral flow, or immunochromatographic, tests were first reported in the early 1980 s2 and were commercially launched by Unipath in 1984 with the first product being a urine-based pregnancy test3. Since then, hundreds of lateral flow assays have been reported and commercialised with applications for detection of infectious diseases, cancer, cardiac diseases, toxins, pathogens, pesticides and metal ions as well as for pharmaceuticals and drugs, as has been reviewed extensively45.…”

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confidence: 99%

Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

Jauset‐Rubio

Svobodová

Mairal

et al. 2016

Sci Rep

141

115

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Sensitive, specific, rapid, inexpensive and easy-to-use nucleic acid tests for use at the point-of-need are critical for the emerging field of personalised medicine for which companion diagnostics are essential, as well as for application in low resource settings. Here we report on the development of a point-of-care nucleic acid lateral flow test for the direct detection of isothermally amplified DNA. The recombinase polymerase amplification method is modified slightly to use tailed primers, resulting in an amplicon with a duplex flanked by two single stranded DNA tails. This tailed amplicon facilitates detection via hybridisation to a surface immobilised oligonucleotide capture probe and a gold nanoparticle labelled reporter probe. A detection limit of 1 × 10−11 M (190 amol), equivalent to 8.67 × 105 copies of DNA was achieved, with the entire assay, both amplification and detection, being completed in less than 15 minutes at a constant temperature of 37 °C. The use of the tailed primers obviates the need for hapten labelling and consequent use of capture and reporter antibodies, whilst also avoiding the need for any post-amplification processing for the generation of single stranded DNA, thus presenting an assay that can facilely find application at the point of need.

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mentioning

confidence: 99%

Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

Jauset‐Rubio

Svobodová

Mairal

et al. 2016

Sci Rep

141

115

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“…The consequence of improved strips is an extended development of more advanced readout instruments versions of readout techniques, thus allowing lateral flow strips to become part of the Binternet of things^as an attractive and costeffective analytical platform for applications in the areas of clinical diagnostics and food and even water safety [8]. Such systems are sometimes called Bmicrofluidic paper-based analytical devices.^Reviews have been published in the fields of measuring pharmaceuticals, point-of-care diagnostics, and bioterrorism detection.…”

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confidence: 99%

ABC Spotlight on paper-based strips analytics

Gauglitz

2017

Anal Bioanal Chem

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“…Within the number of novel materials for labeling antibodies in ICTS, the request for analytical devices with multiplexing capability is prompting towards using labels easily distinguishable from each other, such as nanoparticles with different colors [19][20]23]. Gold nanoparticles are widely employed as labels for ICST, thanks to well-recognized advantages, such as easy preparation, stability, easy conjugation to antibodies, brilliant and tunable color [6][7][8]45]. In this paper, two kinds of GNP differing for the color were prepared and used in combination to set a simple and rapid ICST for determining two mycotoxins simultaneously.…”

Section: Resultsmentioning

confidence: 99%

“…These nanoparticles exhibit a deep-red color, which reflects their SPR band around 525 nm [6][7][8][9] and allow for the naked-eye detection of ICST result when accumulated on the lines. Usually, the visual inspection of the color of the lines provides a qualitative result ("yes/no" response, fig.…”

Section: Introductionmentioning

confidence: 99%

“…Usually, the visual inspection of the color of the lines provides a qualitative result ("yes/no" response, fig. 1); even if semi-quantitative tests can be designed, as well [8][9]. Besides common spherical GNPs, some authors reported the use of non-spherical [10][11] and multilayer GNPs [12][13] as labels for ICSTs with the aim of improving the assay sensitivity More specifically, Ji et al used multi-branched GNPs as labels for developing an ICST for the detection of Aflatoxin B1, and reported higher optical brightness, better colloidal stability and better sensitivity than using s-GNPs [11].…”

Section: Introductionmentioning

confidence: 99%

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Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins

et al. 2017

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Graphical abstractBlue desert rose-like gold nanoparticles (DR-GNPs) were synthesized, characterized and applied as label for the ImmunoChromatographic Strip Test (ICST) technique, in which red spherical GNPs (sGNPs) are usually employed. The combined use of the blue DR-GNP and red s-GNPs allowed developing of an intuitive multicolor ICST for the simultaneous detection of Aflatoxin B1 and AbstractDesert rose-like gold nanoparticles (DR-GNPs) that exhibited a blue color due to the plasmon resonance band at 620 nm were prepared. The blue DR-GNPs were obtained through a seeding growth approach and characterized by UV-vis spectroscopy, transmission electron microscopy and dynamic light scattering. The DR-GNPs had a hydrodynamic diameter of ~72 nm and were used as labels for the antibody in an immunochromatographic strip test (ICST). Although the particular shape of DR-GNPs and their higher surface area with respect to spherical GNPs, they demonstrated to be effective as antibody labels. A multicolor ICST for aflatoxin B1 and fumonisins was developed that employs both blue DR-and red spherical GNPs. The multicolor ICST allows for simultaneous rapid determination of the two mycotoxins in maize flour, with visual cut-off levels at 2 µg kg -1 for aflatoxin B1 and 1000 µg kg -1 for fumonisins.

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Lateral-flow technology: From visual to instrumental

Cited by 217 publications

References 91 publications

Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

ABC Spotlight on paper-based strips analytics

Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins

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