Lateral and Vertical Flow Assays for Point‐of‐Care Diagnostics

Jiang, Nan; Ahmed, Rajib; Damayantharan, Mylon; Ünal, Barış; Butt, Haider; Yetisen, Ali K.

doi:10.1002/adhm.201900244

Cited by 131 publications

(120 citation statements)

References 97 publications

(117 reference statements)

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“…Lateral flow biosensors have been introduced for detection of proteins, mRNA, DNA, miRNA, heavy metals, chemical contaminants, pesticides and biological agents [17][18][19][20][21] . Detection of intact virus, i.e.…”

mentioning

confidence: 99%

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Toubanaki

Margaroni

Prapas³

et al. 2020

Sci Rep

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Effective analysis of pathogens causing human and veterinary diseases demands rapid, specific and sensitive detection methods which can be applied in research laboratory setups and in field for routine diagnosis. Paper lateral flow biosensors (LFBs) have been established as attractive tools for such analytical applications. In the present study a prototype LFB was designed for whole particles (virions) detection of nodavirus or fish nervous necrosis virus. Nodavirus is an important threat in the aquaculture industry, causing severe economic losses and environmental problems. The LFB was based on polyclonal antibodies conjugated on gold nanoparticles for signal visualization. Brain and retinas from fish samples were homogenized, centrifuged and the supernatant was directly applied on the LFB. formation of a red test line was indicative of nodavirus virions presence. nodavirus visual detection was completed in short time (30 min). Key factors of the LFB development influencing the assays' detection limit were characterized and the optimum parameters were determined, enabling increased efficiency, excluding non-specific interactions. Therefore, the proposed LFB assay consists a robust, simple, low cost and accurate method for detection of nodavirus virions in fish samples. The proposed biosensor is ideal for development of a commercial kit to be used on aquaculture facilities by fish farmers. It is anticipated that disease monitoring and environmental safety will benefit from the simplification of time consuming and costly procedures.Aquaculture is essential to cover fish-product demands, providing seafood in high quantities, and covering more than the half amount of fish consumed worldwide. This fact drives a strong demand for high production efficiency in aquaculture industry in order to cover the feeding needs of the world's growing population, in the middle of an increasing environmental crisis 1,2 . As a result, the aquaculture industry has continuously increased profits in a high rate. However, outbreaks of diseases caused by infectious agents are significantly restricting intensified aquaculture. According to literature 3 , 22.6% of all disease outbreaks are caused by viruses. Among these, viral nervous necrosis (VNN), also named vacuolating encephalopathy and retinopathy or encephalomyelitis, is a devastating disease, which induces cell necrosis accompanied by vacuolation in fish retina and brain. Its clinical symptoms include changes in skin color with abnormal swimming, low feed ingestion and altered buoyancy in affected fish. The disease is caused by nervous necrosis virus (NNV) or nodavirus, affecting more than 30 different fish species, worldwide. VNN causes high mortalities (80-100% in several species e.g. European sea bass), emerging as a major problem especially in the Mediterranean area, since it cannot be prevented by vaccination or effective treatment 4-6 .Fish nervous necrosis virus (belonging to Betanodavirus genus and Nodaviridae family) is icosahedral, and non-enveloped (∼25 nm in diameter). I...

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confidence: 99%

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Toubanaki

Margaroni

Prapas³

et al. 2020

Sci Rep

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“…This substrate is typically composed of individual or stacked paper-based layers and operates similarly to an LFA. VFAs offer several significant advantages over LFAs as the vertical flow of fluid leads to shorter assay times, no flow time requirement, and better multiplexing capability [ 40 ].…”

Section: Biosensors To the Rescuementioning

confidence: 99%

Lyme Disease Biosensors: A Potential Solution to a Diagnostic Dilemma

Flynn

Ignaszak

2020

Biosensors

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Over the past four decades, Lyme disease has remained a virulent and pervasive illness, persisting throughout North America and many other regions of the world. Recent increases in illness in many countries has sparked a renewed interest in improved Lyme diagnostics. While current standards of diagnosis are acceptable for the late stages of the disease, it remains difficult to accurately diagnose early forms of the illness. In addition, current diagnostic methods tend to be relatively expensive and require a large degree of laboratory-based analysis. Biosensors represent the fusion of biological materials with chemical techniques to provide simple, inexpensive alternatives to traditional diagnostic methods. Lyme disease biosensors have the potential to better diagnose early stages of the illness and provide possible patients with an inexpensive, commercially available test. This review examines the current state of Lyme disease biosensing, with a focus on previous biosensor development and essential future considerations.

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“…Conventional LFA provides "yes or no" results by inspection of color changes on the test line by the naked eye, the most popular method of detection for these kinds of assays. Thus, this type of approach tends to suffer from a lack of accuracy and subjective judgment [8]. Nonetheless, since it is easy to integrate LFA with electronic devices, a feasible detection approach is to develop strip readers in order to obtain accurate quantitative results.…”

Section: Introductionmentioning

confidence: 99%

A plasmonic thermal sensing based portable device for lateral flow assay detection and quantification

Wang

Alfranca

et al. 2020

Nanoscale Res Lett

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Point-of-care testing (POCT) is widely used for early diagnosis and monitoring of diseases. Lateral flow assay (LFA) is a successfully commercial tool for POCT. However, LFA often suffers from a lack of quantification and analytical sensitivity. To solve these drawbacks, we have previously developed a thermal LFA using plasmonic gold nanoparticles for thermal contrast into a portable device. Although this methodology significantly improves the analytical sensitivity compared with conventional visual detection, quantification problems are still remaining. In this study, we optimized the operating conditions for the device using conduction and radiation thermal sensing modes allowing the quantification of LFA. The limit of detection of the strips merely containing nanoparticles was decreased by 5-fold (conduction mode) and 12-fold (radiation mode) compared to traditional visual detection. The effect of the ambient temperature was studied for both methods of detection showing that the radiation mode was more affected by the ambient temperature than the conduction mode. To validate the thermal sensing method, human chorionic gonadotropin (HCG) biomarker was quantified using our LFA strips, obtaining a detection limit of 2.8 mIU/mL when using the radiation method of detection.

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Lateral and Vertical Flow Assays for Point‐of‐Care Diagnostics

Cited by 131 publications

References 97 publications

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Development of a Nanoparticle-based Lateral Flow Strip Biosensor for Visual Detection of Whole Nervous Necrosis Virus Particles

Lyme Disease Biosensors: A Potential Solution to a Diagnostic Dilemma

A plasmonic thermal sensing based portable device for lateral flow assay detection and quantification

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