Laser raman spectroscopy of calf bone Gla protein

Dendramis, A.; Poser, James W.; Schwinn, Edward W.

doi:10.1016/0167-4838(83)90270-4

Cited by 5 publications

(1 citation statement)

References 20 publications

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“…Noncollagenous proteins (NCPs) almost certainly contribute to the bone Raman spectrum [19], but their low abundance and spectral similarity with collagen [19,57] make them difficult to isolate spectroscopically in whole bone specimens. In contrast, fluorescent blood products in bone cause intense background features, which can obscure bone Raman spectra [68].…”

Section: Introductionmentioning

confidence: 99%

Raman Assessment of Bone Quality

Morris

Mandair

2011

Clinical Orthopaedics &Amp; Related Research

439

477

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Background Progress in the diagnosis and prediction of fragility fractures depends on improvements to the understating of the compositional contributors of bone quality to mechanical competence. Raman spectroscopy has been used to evaluate alterations to bone composition associated with aging, disease, or injury. Questions/purposes In this survey we will (1) review the use of Raman-based compositional measures of bone quality, including mineral-to-matrix ratio, carbonateto-phosphate ratio, collagen quality, and crystallinity; (2) review literature correlating Raman spectra with biomechanical and other physiochemical measurements and with bone health; and (3) discuss prospects for ex vivo and in vivo human subject measurements. Methods ISI Web of Science was searched for references to bone Raman spectroscopy in peer-reviewed journals. Papers from other topics have been excluded from this review, including those on pharmaceutical topics, dental tissue, tissue engineering, stem cells, and implant integration. Results Raman spectra have been reported for human and animal bone as a function of age, biomechanical status, pathology, and other quality parameters. Current literature supports the use of mineral-to-matrix ratio, carbonate-tophosphate ratio, and mineral crystallinity as measures of bone quality. Discrepancies between reports arise from the use of band intensity ratios rather than true composition ratios, primarily as a result of differing collagen band selections. Conclusions Raman spectroscopy shows promise for evaluating the compositional contributors of bone quality in ex vivo specimens, although further validation is still needed. Methodology for noninvasive in vivo assessments is still under development.

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