Laser-Induced Primate Glaucoma

Altered F-actin distribution in retinal nerve fiber layer of a rat model of glaucoma

“…Selective change of axonal F-actin in dorsal retina is consistent with selective loss of RGCs or axons found in rodent models of glaucoma by other studies (Johnson et al, 2000; Mabuchi et al, 2004; Morrison et al, 1997; Villegas-Perez et al, 2005). This pattern, however, is different than that found in primate eyes, in which early damage of RNFL appears in both superior and inferior regions (Pederson and Gassterland, 1984; Quigley and Addicks, 1982; Quigley et al, 1981; Sommer et al, 1991). The difference has been suggested to be due to anatomic differences between rodents and primates (Mabuchi et al, 2004; Morrison et al, 1997).…”

Altered F-actin distribution in retinal nerve fiber layer of a rat model of glaucoma

“…Selective change of axonal F-actin in dorsal retina is consistent with selective loss of RGCs or axons found in rodent models of glaucoma by other studies (Johnson et al, 2000; Mabuchi et al, 2004; Morrison et al, 1997; Villegas-Perez et al, 2005). This pattern, however, is different than that found in primate eyes, in which early damage of RNFL appears in both superior and inferior regions (Pederson and Gassterland, 1984; Quigley and Addicks, 1982; Quigley et al, 1981; Sommer et al, 1991). The difference has been suggested to be due to anatomic differences between rodents and primates (Mabuchi et al, 2004; Morrison et al, 1997).…”

Biomechanical, ultrastructural, and electrophysiological characterization of the non-human primate experimental glaucoma model

“…In the animals in this study, sustained increase in IOP was successfully established by inducing subtotal fibrosis of the TM, effectively reducing outflow area. In these animals, in vivo OCT of the fundus confirmed the presence of characteristic features of ExGl including cupping of the ONH, neuroretinal rim thinning, posterior displacement of the lamina cribrosa, and decreased RNFL thickness, indicative of RGC loss 25 – 28 . As with humans, NHPs with advanced stages of glaucoma tend to exhibit a “floor effect” in SD-OCT derived measurements.…”

Efficacy, Drug Sensitivity, and Safety of a Chronic Ocular Hypertension Rat Model Established Using a Single Intracameral Injection of Hydrogel into the Anterior Chamber

“…Various COH models have been established in rodents. In addition to the well-known and widely used methods, like gene mutation [ 20 ], other methods have become important, such as laser photocoagulation of the trabecular meshwork at the limbus [ 21 , 22 ], episcleral veins injection of hypertonic saline [ 6 , 7 ], occlusion of the episcleral veins by cauterization or suture ligation [ 9 , 23 ], and injection of microbead [ 11 , 24 , 25 ] or hyaluronic acid into the anterior chamber [ 13 ]. Other new rodent COH models, such as circumlimbal suture [ 26 ] and intracameral injection of conjunctival fibroblasts [ 27 ], have also been reported in recent years.…”

Altered F-actin distribution in retinal nerve fiber layer of a rat model of glaucoma

“…Selective change of axonal F-actin in dorsal retina is consistent with selective loss of RGCs or axons found in rodent models of glaucoma by other studies (Johnson et al, 2000; Mabuchi et al, 2004; Morrison et al, 1997; Villegas-Perez et al, 2005). This pattern, however, is different than that found in primate eyes, in which early damage of RNFL appears in both superior and inferior regions (Pederson and Gassterland, 1984; Quigley and Addicks, 1982; Quigley et al, 1981; Sommer et al, 1991). The difference has been suggested to be due to anatomic differences between rodents and primates (Mabuchi et al, 2004; Morrison et al, 1997).…”

Biomechanical, ultrastructural, and electrophysiological characterization of the non-human primate experimental glaucoma model

“…In the animals in this study, sustained increase in IOP was successfully established by inducing subtotal fibrosis of the TM, effectively reducing outflow area. In these animals, in vivo OCT of the fundus confirmed the presence of characteristic features of ExGl including cupping of the ONH, neuroretinal rim thinning, posterior displacement of the lamina cribrosa, and decreased RNFL thickness, indicative of RGC loss 25 – 28 . As with humans, NHPs with advanced stages of glaucoma tend to exhibit a “floor effect” in SD-OCT derived measurements.…”

Efficacy, Drug Sensitivity, and Safety of a Chronic Ocular Hypertension Rat Model Established Using a Single Intracameral Injection of Hydrogel into the Anterior Chamber

“…Various COH models have been established in rodents. In addition to the well-known and widely used methods, like gene mutation [ 20 ], other methods have become important, such as laser photocoagulation of the trabecular meshwork at the limbus [ 21 , 22 ], episcleral veins injection of hypertonic saline [ 6 , 7 ], occlusion of the episcleral veins by cauterization or suture ligation [ 9 , 23 ], and injection of microbead [ 11 , 24 , 25 ] or hyaluronic acid into the anterior chamber [ 13 ]. Other new rodent COH models, such as circumlimbal suture [ 26 ] and intracameral injection of conjunctival fibroblasts [ 27 ], have also been reported in recent years.…”

Altered F-actin distribution in retinal nerve fiber layer of a rat model of glaucoma

“…Selective change of axonal F-actin in dorsal retina is consistent with selective loss of RGCs or axons found in rodent models of glaucoma by other studies (Johnson et al, 2000; Mabuchi et al, 2004; Morrison et al, 1997; Villegas-Perez et al, 2005). This pattern, however, is different than that found in primate eyes, in which early damage of RNFL appears in both superior and inferior regions (Pederson and Gassterland, 1984; Quigley and Addicks, 1982; Quigley et al, 1981; Sommer et al, 1991). The difference has been suggested to be due to anatomic differences between rodents and primates (Mabuchi et al, 2004; Morrison et al, 1997).…”

Biomechanical, ultrastructural, and electrophysiological characterization of the non-human primate experimental glaucoma model

“…In the animals in this study, sustained increase in IOP was successfully established by inducing subtotal fibrosis of the TM, effectively reducing outflow area. In these animals, in vivo OCT of the fundus confirmed the presence of characteristic features of ExGl including cupping of the ONH, neuroretinal rim thinning, posterior displacement of the lamina cribrosa, and decreased RNFL thickness, indicative of RGC loss 25 – 28 . As with humans, NHPs with advanced stages of glaucoma tend to exhibit a “floor effect” in SD-OCT derived measurements.…”

Efficacy, Drug Sensitivity, and Safety of a Chronic Ocular Hypertension Rat Model Established Using a Single Intracameral Injection of Hydrogel into the Anterior Chamber

“…Various COH models have been established in rodents. In addition to the well-known and widely used methods, like gene mutation [ 20 ], other methods have become important, such as laser photocoagulation of the trabecular meshwork at the limbus [ 21 , 22 ], episcleral veins injection of hypertonic saline [ 6 , 7 ], occlusion of the episcleral veins by cauterization or suture ligation [ 9 , 23 ], and injection of microbead [ 11 , 24 , 25 ] or hyaluronic acid into the anterior chamber [ 13 ]. Other new rodent COH models, such as circumlimbal suture [ 26 ] and intracameral injection of conjunctival fibroblasts [ 27 ], have also been reported in recent years.…”