Laser ablation inductively coupled plasma-mass spectrometry in combination with gel electrophoresis: a new strategy for speciation of metal binding serum proteins

Neilsen, J.L.; Abildtrup, Anne; Christensen, Jennie R.; Watson, Paul; Cox, Alan G.; McLeod, Cameron W.

doi:10.1016/s0584-8547(98)00077-9

Cited by 119 publications

(106 citation statements)

References 10 publications

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“…ICP-MS is a powerful and highly sensitive technique for elemental analysis, and has been widely used when interfaced with HPLC for the analysis of metals in biological samples (30,31). Combined with laser ablation, ICP-MS has been used to analyse metallo-, seleno-or phospho-proteins resolved by PAGE (26,27,32,33).…”

Section: Discussionmentioning

confidence: 99%

The role of cisplatin and NAMI-A plasma-protein interactions in relation to combination therapy

Khalaila¹,

Bergamo²,

Bussy³

et al. 2006

Int J Oncol

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Abstract. The aim of the study is to evaluate the differences of protein binding of NAMI-A, a new ruthenium drug endowed with selective antimetastatic properties, and of cisplatin and to ascertain the possibility to use two drugs based on heavy metals in combination to treat solid tumour metastases. For this purpose, we have developed a technique that allows the proteins, to which metal drugs bind, to be identified from real protein mixtures. Following incubation with the drugs, the bands containing platinum and/or ruthenium are separated by native PAGE, SDS-PAGE and 2D gel electrophoresis, and identified using laser ablation inductively coupled plasma mass spectrometry. Both drugs interact with essentially the same proteins which, characterised by proteomics, are human serum albumin precursor, macroglobulin ·2 and human serotransferrin precursor. The interactions of NAMI-A are largely reversible whereas cisplatin forms stronger interactions that are less reversible. These data correlate well with the MCa mammary carcinoma model on which full doses of NAMI-A combined with cisplatin show additive effects as compared to each treatment taken alone, independently of whether NAMI-A precedes or follows cisplatin. Furthermore, the implication from this study is that the significantly lower toxicity of NAMI-A, compared to cisplatin, could be a consequence of differences in the mode of binding to plasma proteins, involving weaker interactions compared to cisplatin.

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Section: Discussionmentioning

confidence: 99%

The role of cisplatin and NAMI-A plasma-protein interactions in relation to combination therapy

Khalaila¹,

Bergamo²,

Bussy³

et al. 2006

Int J Oncol

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“…This is an important limitation to the method, since the use of radioactive material with humans is restricted and since a radionuclide with a suitable half-life not always exists. Various alternative methods for the detection of the metals in the gel have been applied (see [1] for a review), among which laser ablation (LA) is the most recent [2,3].…”

Section: Introductionmentioning

confidence: 99%

Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation‐inductively coupled plasma‐mass spectrometry: Application to selenium

et al. 2003

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The capabilities of laser ablation-inductively coupled plasma-mass spectrometry for the detection of trace elements in a gel after gel electrophoresis were systematically studied. Figures of merit, such as limit of detection, linearity, and repeatability, were evaluated for various elements (Li, V, Cr, Mn, Ni, Cu, Zn, As, Se, Mo, Pd, Ag, Cd, Pt, Tl, Pb). Two ablation strategies were followed: single hole drilling, relevant for ablation of spots after two-dimensional (2-D) separations, and ablation with translation, i.e., on a line, relevant for one-dimensional (1-D) separations. This technique was applied to the detection of selenoproteins in red blood cells extracts after a 1-D separation (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and the detection of selenium-containing proteins in yeast after 2-D electrophoresis (2-DE). The detection procedure was further improved by using the dynamic reaction cell technology, which allowed the removal of the Ar 2 interference and hence the use of the most abundant Se isotope, 80 Se. Reaction gases were compared (methane, carbon monoxide, ammonia, oxygen and the combination of argon (collision gas) and hydrogen (reaction gas)). In each instance, the reaction cell parameters were optimized in order to obtain the lowest detection limit for Se (as 80 Se 16 O 1 with O 2 as the reaction gas). Carbon monoxide was found to offer the best performance. The detection limit with the use of DRC and He as transport gas was 0.07 mg Se g 21 gel with single hole drilling and 0.15 mg Se g 21 gel for ablation with translation.

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“…LA-ICP-MS imaging, pioneered by Nielsen et al [51], offers a competitive alternative for the in situ probing of the protein spots for the presence of metals and metalloids. The ablated analytes are swept into the ICP by a continuous stream of argon, and the ions are analyzed by MS. As a result, an electro pherogram is obtained in which the quantity of a given element is a function of its position in the gel.…”

Section: Gel Electrophoresis With Element-specific Detectionmentioning

confidence: 99%

Metallomics: Guidelines for terminology and critical evaluation of analytical chemistry approaches (IUPAC Technical Report)

Łobiński

Becker

Haraguchi

et al. 2010

Pure and Applied Chemistry

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Definitions for the terms "metallome" and "metallomics" are proposed. The state of the art of analytical techniques and methods for systematic studies of metal content, speciation, localization, and use in biological systems is briefly summarized and critically evaluated.Keywords: metals; metabolomics; metallomics; proteomics; speciation. RATIONALE AND HISTORY OF THE USE OF THE TERMS REGARDING THE METAL-RELATED -OMICSThe knowledge of the complete genetic blueprint of an increasing number of organisms has resulted in efforts aimed at the global analysis and functional study of a particular class of components of a living organism and the emergence of different "-omics". The concepts of genomics (the study of genes and their function) and proteomics (the study of the set of proteins produced by an organism, their localization, structure, stability, and interaction) have become part of the everyday language of life sciences [1,2].Metal ions are a vital component of the chemistry of life [3]. One-third of all proteins is believed to require a metal cofactor, such as copper, iron, zinc, or molybdenum [4], delivered as a simple or complex ion or a metal-containing compound (e.g., methylcobalamin). The intracellular concentration of several metals, their distribution among the various cell compartments, and their incorporation in metallo proteins are tightly controlled [5]. The understanding of mechanisms by which a metal is sensed, stored, or incorporated as a cofactor requires, in addition to the identification of metalloproteins, the characterization of the pool of non-protein molecules (products of enzymatic or biochemical reactions) interacting with metal ions or of metabolites of exogenous metallocompounds, such as metallodrugs. A systematic approach to the study of metal content, speciation, localization, and use in biological systems is becoming increasingly important [6].

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Laser ablation inductively coupled plasma-mass spectrometry in combination with gel electrophoresis: a new strategy for speciation of metal binding serum proteins

Cited by 119 publications

References 10 publications

The role of cisplatin and NAMI-A plasma-protein interactions in relation to combination therapy

The role of cisplatin and NAMI-A plasma-protein interactions in relation to combination therapy

Detection of metals in proteins by means of polyacrylamide gel electrophoresis and laser ablation‐inductively coupled plasma‐mass spectrometry: Application to selenium

Metallomics: Guidelines for terminology and critical evaluation of analytical chemistry approaches (IUPAC Technical Report)

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