Larvicidal and ovicidal activity of carvacrol, p-cymene, and γ-terpinene from Origanum vulgare essential oil against the cotton bollworm, Helicoverpa armigera (Hübner)

Gong, Xue; Ren, Yujian

doi:10.1007/s11356-020-08391-2

Cited by 43 publications

(24 citation statements)

References 77 publications

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“…bevanii) exhibited antimicrobial activities against a variety of plant pathogenic fungi and bacteria (Table 1). In addition, they had excellent effects on the control of agricultural insect pests (Gong & Ren, 2020;Pavela, 2012), stored product insects (Ebadollahi, Ziaee & Palla, 2020) and acarids (Koc et al, 2013;Zandi-Sohani & Ramezani, 2015;Shang et al, 2016). Interestingly, although many of Origanum species shared the same ingredients, it showed significant differences to the same microorganism (De Martino, De Feo & Nazzaro, 2009b;Mamadalieva et al, 2017;Moumni et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Effects of Origanum vulgare essential oil and its two main components, carvacrol and thymol, on the plant pathogen Botrytis cinerea

Hou

Zhang

Zhao

et al. 2020

PeerJ

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Background Botrytis cinerea causes serious gray mold disease in many plants. This pathogen has developed resistance to many fungicides. Thus, it has become necessary to look for new safe yet effective compounds against B. cinerea. Methods Essential oils (EOs) from 17 plant species were assayed against B. cinerea, of which Origanum vulgare essential oil (OVEO) showed strong antifungal activity, and accordingly its main components were detected by GC/MS. Further study was conducted on the effects of OVEO, carvacrol and thymol in vitro on mycelium growth and spore germination, mycelium morphology, leakages of cytoplasmic contents, mitochondrial injury and accumulation of reactive oxygen species (ROS) of B. cinerea. The control efficacies of OVEO, carvacrol and thymol on tomato gray mold were evaluated in vivo. Results Of all the 17 plant EOs tested, Cinnamomum cassia, Litsea cubeba var. formosana and O. vulgare EOs had the best inhibitory effect on B. cinerea, with 0.5 mg/mL completely inhibiting the mycelium growth of B. cinerea. Twenty-one different compounds of OVEO were identified by gas chromatography–mass spectrometry, and the main chemical components were carvacrol (89.98%), β-caryophyllene (3.34%), thymol (2.39%), α-humulene (1.38%) and 1-methyl-2-propan-2-ylbenzene isopropyl benzene (1.36%). In vitro experiment showed EC50 values of OVEO, carvacrol and thymol were 140.04, 9.09 and 21.32 μg/mL, respectively. Carvacrol and thymol completely inhibited the spore germination of B. cinerea at the concentration of 300 μg/mL while the inhibition rate of OVEO was 80.03%. EC50 of carvacrol and thymol have significantly (P < 0.05) reduced the fresh and dry weight of mycelia. The collapse and damage on B. cinerea mycelia treated with 40 μg/mL of carvacrol and thymol was examined by scanning electron microscope (SEM). Through extracellular conductivity test and fluorescence microscope observation, it was found that carvacrol and thymol led to increase the permeability of target cells, the destruction of mitochondrial membrane and ROS accumulation. In vivo conditions, 1000 μg/mL carvacrol had the best protective and therapeutic effects on tomato gray mold (77.98% and 28.04%, respectively), and the protective effect was significantly higher than that of 400 μg/mL pyrimethanil (43.15%). While the therapeutic and protective effects of 1,000 μg/mL OVEO and thymol were comparable to chemical control. Conclusions OVEO showed moderate antifungal activity, whereas its main components carvacrol and thymol have great application potential as natural fungicides or lead compounds for commercial fungicides in preventing and controlling plant diseases caused by B. cinerea.

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Section: Introductionmentioning

confidence: 99%

Effects of Origanum vulgare essential oil and its two main components, carvacrol and thymol, on the plant pathogen Botrytis cinerea

Hou

Zhang

Zhao

et al. 2020

PeerJ

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“…In addition, these chemical agents present a high risk of toxicity to the farmers and farm workers. Morevover, their intensive and indiscriminate applications have caused the emergence of new pests and resistant populations (Camaroti et al 2018;Gong and Ren 2020). The increasing resistance of many storage insects, including S. granarius, against a variety of insecticides, such as deltamethrin, malathion, tetrachlorvinphos, fenitrothion, and phosphine, has been reported (Tyler & Binns 1982;Kljajic & Ilija 2005;Mutlu et al 2019).…”

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confidence: 99%

Toxicity and repellent activities of Thymus pallescens and Cymbopogon citratus essential oils against Sitophylus granarius

Moutassem¹,

Bellik²,

Sannef³

2021

Plant Prot. Sci.

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This study evaluated the toxicity and repellent activities of essential oils (EOs) against Sitophilus granarius (Linnaeus, 1758), both in vitro and in vivo conditions. The EOs obtained from Thymus pallescens Noë. (Lamiaceae) and Cymbopogon citratus Stapf. (Poaceae) were analyzed by GC-MS, and revealed that carvacrol (56.64%) and geraniol (20.8%) as the respective major components. T. pallescens EO was found to be a more effective toxicant, with LC<sub>50</sub> and LC<sub>90</sub> values of 9.3 and 34.6 µL/mL, respectively, in the contact test vs 8.2 and 25.3 µL/mL in the fumigation test. T. pallescens EO showed also a stronger repellent effect with values ranging from 83.4% to 100%. In the in vivo test, the survival probability decreased from 99.9 to 2.48% among insects exposed to T. pallescens EO. These data demonstrated that EOs could be considered effective alternatives to chemical insecticides, providing pest control for stored products in an ecologically sustainable manner.

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“…It is generally known that the fungicidal, insecticidal, and nematocidal activities of plant EOs can be attributed to various compounds, notably alcohols, aldehydes, phenol, terpenes, and terpenoids (Boulogne et al 2012;Tak and Isman 2017;Benelli et al 2018;Liu et al 2019;Gong and Ren 2020). In previous studies, the chemical analysis of litsea, melissa, palmarosa, and verbena has been reported (Seo et al 2009;De Martino et al 2011;Si et al 2012;Kakaraparthi et al 2015;Rehman et al 2017;Khalili et al 2018;Kittler et al 2018a,b;Pouyanfar et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Antifungal activity and mechanism of the essential oils from Litsea (Litsea cubeba), Melissa (Melissa officinalis), Palmarosa (Cymbopogon martini) and Verbena (Verbena officinalis) and their major active constituents against Trametes hirsuta and Laetiporus sulphureus

Xie

Yang

Han

et al. 2021

Preprint

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Antifungal activities of 37 essential oils (EOs) against two wood-decaying fungi, Trametes hirsuta and Laetiporus sulphureus were screened in vitro, and investigated the underlying mechanism. Of the 37 EOs, litsea (Litsea cubeba), melissa (Melissa officinalis), palmarosa (Cymbopogon martini), and verbena (Verbena officinalis) demonstrated strong antifungal activity, in which litsea oil exhibited the strongest antifungal property against T. hirsuta and L. sulphureus, with IC50 values of 72.3 and 40.2 µg/ml, respectively. The compositions of litsea, melissa, palmarosa, and verbena EOs were analyzed using a gas chromatography-mass spectrometry method and demonstrated geranial, geraniol, neral, and citral as their major active constituents. Among of them geranial exhibited the strongest antifungal activity against T. hirsuta and L. sulphureus, with IC50 values of 56.6 and 33.3 µg/ml, respectively. These EOs and their major active constituents increased the plasma membrane permeability of T. hirsuta and L. sulphureus, resulting in the leakage of nucleic acid, protein, and soluble sugar. Results indicate that the EOs of litsea, melissa, palmarosa, and verbena and its major constituents inhibited T. hirsuta and L. sulphureus growth by targeting its plasma membrane.

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Larvicidal and ovicidal activity of carvacrol, p-cymene, and γ-terpinene from Origanum vulgare essential oil against the cotton bollworm, Helicoverpa armigera (Hübner)

Cited by 43 publications

References 77 publications

Effects of Origanum vulgare essential oil and its two main components, carvacrol and thymol, on the plant pathogen Botrytis cinerea

Effects of Origanum vulgare essential oil and its two main components, carvacrol and thymol, on the plant pathogen Botrytis cinerea

Toxicity and repellent activities of Thymus pallescens and Cymbopogon citratus essential oils against Sitophylus granarius

Antifungal activity and mechanism of the essential oils from Litsea (Litsea cubeba), Melissa (Melissa officinalis), Palmarosa (Cymbopogon martini) and Verbena (Verbena officinalis) and their major active constituents against Trametes hirsuta and Laetiporus sulphureus

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