Large volume leukapheresis for peripheral blood stem cell collection in children under 10 kg in weight

Peripheral blood stem cell collection in children with extremely low body weight (≤8 kg). What have we learned over the past 25 years and where are the limits?

“…Most teams used central catheters for leukapheresis in very small children 18,20‐27 . In our experience, whenever a central line had previously been inserted, it was always used for collection.…”

“…Pediatric patients with a lower total RBC volume due to small size may not tolerate this reduction in circulating red cell mass 25 . In the literature, most authors report the systematic use of RBC priming 18,20‐22,24‐26 . This may be partly because none of the leukaphereses reported was performed with the Optia device, and because the extracorporeal volume was larger with older devices.…”

Clinical Application of Apheresis in Very Small Dogs Weighing <8 kg to Pediatric Patients

“…Another issue relating to hemodynamic stability is the volume of extracorporeal blood in the machine that is returned to the dogs in the rinseback procedure. For the dogs undergoing LVL, this would be the equivalent of an approximately 285 mL transfusion over 15 min, which would result in a large net positive gain to their TBV . This is also an important issue for TPE since, although the extracorporeal volume of the tubing kit is only ~140 mL, immune‐mediated diseases, such as IMHA, tend to occur primarily in small breed dogs.…”

Hematopoietic progenitor cells collection in pediatric patients with brain tumor

“…The use of a central venous catheter is also regarded as an important problem by some authors . All of our leukapheresis have been performed smoothly by a temporary, tunneled, and rigid catheter, which was removed the day after collection.…”