Large-scale microarray gene expression analysis in discrete electrophysiologically identified neuronal clusters

Bragin, Anatol; Karsten, Stanislav L.; Almajano, Joyel; Wilson, Charles L.; Geschwind, Daniel H.; Engel, Jerome

doi:10.1016/j.jneumeth.2003.09.016

Cited by 2 publications

(2 citation statements)

References 27 publications

(28 reference statements)

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“…As microarrays were the ideal technology for providing genome-wide expression information, [11][12][13] they were employed to detect transcriptomic differences between human and non-human primates. One of the first studies using blood, liver and brain reported that human brain has an accelerated number of gene expression changes compared with chimpanzees, orangutans and macaques.…”

Section: Gene Expression Divergence Among Brain Regionsmentioning

confidence: 99%

Decoding human gene expression signatures in the brain

Wang

Konopka²

2013

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T he evolution of higher cognitive functions in humans is thought to be due, at least in part, to the molecular evolution of gene expression patterns specific to the human brain. In this article, we explore recent and past findings using comparative genomics in human and non-human primate brain to identify these novel human patterns. We suggest additional directions and lines of experimentation that should be taken to improve our understanding of these changes on the human lineage. Finally, we attempt to put into context these genomic changes with biological phenotypes and diseases in humans.

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Section: Gene Expression Divergence Among Brain Regionsmentioning

confidence: 99%

Decoding human gene expression signatures in the brain

Wang

Konopka²

2013

Transcription

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“…However, advances in applying functional genomics techniques on limited biological samples provide the ability to study molecular processes within electrophysiologically identified neuronal clusters (Bragin et al, 2004). Therefore, we designed our study to compare gene expression in areas of the dentate gyrus that generated FRs to neighboring regions that did not display abnormal activity.…”

Section: Introductionmentioning

confidence: 99%

Molecular alterations in areas generating fast ripples in an animal model of temporal lobe epilepsy

Winden

Bragin

Engel

et al. 2015

Neurobiology of Disease

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The molecular basis of epileptogenesis is poorly characterized. Studies in humans and animal models have identified an electrophysiological signature that precedes the onset of epilepsy, which has been termed fast ripples (FRs) based on its frequency. Multiple lines of evidence implicate regions generating FRs in epileptogenesis, and FRs appear to demarcate the seizure onset zone, suggesting a role in ictogenesis as well. We performed gene expression analysis comparing areas of the dentate gyrus that generate FRs to those that do not generate FRs in a well-characterized rat model of epilepsy. We identified a small cohort of genes that are differentially expressed in FR versus non-FR brain tissue and used quantitative PCR to validate some of those that modulate neuronal excitability. Gene expression network analysis demonstrated conservation of gene co-expression between non-FR and FR samples, but examination of gene connectivity revealed changes that were most pronounced in the cm-40 module, which contains several genes associated with synaptic function and the differentially expressed genes Kcna4, Kcnv1, and Npy1r that are down-regulated in FRs. We then demonstrate that the genes within the cm-40 module are regulated by seizure activity and enriched for the targets of the RNA binding protein Elavl4. Our data suggest that seizure activity induces co-expression of genes associated with synaptic transmission and that this pattern is attenuated in areas displaying FRs, implicating the failure of this mechanism in the generation of FRs.

show abstract

Large-scale microarray gene expression analysis in discrete electrophysiologically identified neuronal clusters

Cited by 2 publications

References 27 publications

Decoding human gene expression signatures in the brain

Decoding human gene expression signatures in the brain

Molecular alterations in areas generating fast ripples in an animal model of temporal lobe epilepsy

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