Large sample volumes in preparative chromatography

Characterization and applications of Amberlite XAD-4 in preparative liquid chromatography

“…Volume Overload. The effect of volume overload on peak shape has been widely studied by using silica-based preparative columns (2,3,17,18). In general, an evaluation of the effect of injection volume on both the 8.0 mm and 20.5 mm i.d.…”

Characterization and applications of Amberlite XAD-4 in preparative liquid chromatography

“…Volume Overload. The effect of volume overload on peak shape has been widely studied by using silica-based preparative columns (2,3,17,18). In general, an evaluation of the effect of injection volume on both the 8.0 mm and 20.5 mm i.d.…”

Characterization of pectins by size exclusion chromatography in conjunction with viscosity detection

“…An elution band with a plateau can be produced by adopting the procedure of Barford et al (1978): A small sample (e.g. 100 µ ) is injected onto the column and the retention volume of the eluted peak width measured (ca.…”

Process Considerations for Scale-up of Liquid Chromatography and Electrophoresis

“…This phenomena is reflected by an increase in peak standard deviation (σ) where the width of a Gaussian peak is 4σ. In process chromatography, particularly in the case of sample overload, the peak may be skewed (16). A standard deviation can still be defined leading to a value of plate height, although moment analysis of the eluting peak must be used to obtain values of σ (5,17).…”

Characterization and applications of Amberlite XAD-4 in preparative liquid chromatography

“…Volume Overload. The effect of volume overload on peak shape has been widely studied by using silica-based preparative columns (2,3,17,18). In general, an evaluation of the effect of injection volume on both the 8.0 mm and 20.5 mm i.d.…”

Characterization of pectins by size exclusion chromatography in conjunction with viscosity detection

“…An elution band with a plateau can be produced by adopting the procedure of Barford et al (1978): A small sample (e.g. 100 µ ) is injected onto the column and the retention volume of the eluted peak width measured (ca.…”

Process Considerations for Scale-up of Liquid Chromatography and Electrophoresis

“…This phenomena is reflected by an increase in peak standard deviation (σ) where the width of a Gaussian peak is 4σ. In process chromatography, particularly in the case of sample overload, the peak may be skewed (16). A standard deviation can still be defined leading to a value of plate height, although moment analysis of the eluting peak must be used to obtain values of σ (5,17).…”

Characterization and applications of Amberlite XAD-4 in preparative liquid chromatography

“…Volume Overload. The effect of volume overload on peak shape has been widely studied by using silica-based preparative columns (2,3,17,18). In general, an evaluation of the effect of injection volume on both the 8.0 mm and 20.5 mm i.d.…”

Characterization of pectins by size exclusion chromatography in conjunction with viscosity detection

“…An elution band with a plateau can be produced by adopting the procedure of Barford et al (1978): A small sample (e.g. 100 µ ) is injected onto the column and the retention volume of the eluted peak width measured (ca.…”

Process Considerations for Scale-up of Liquid Chromatography and Electrophoresis

“…This phenomena is reflected by an increase in peak standard deviation (σ) where the width of a Gaussian peak is 4σ. In process chromatography, particularly in the case of sample overload, the peak may be skewed (16). A standard deviation can still be defined leading to a value of plate height, although moment analysis of the eluting peak must be used to obtain values of σ (5,17).…”