Large offspring syndrome in cattle and sheep

Young, Lorraine; Sinclair, Kevin D.; Wilmut, Ian

doi:10.1530/ror.0.0030155

Cited by 785 publications

(287 citation statements)

References 16 publications

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“…Several studies have demonstrated increased blastocyst development when FBS is supplemented, (Lazzari et al 2002, Leivas et al 2011) similar to the current study. However, the use of FBS has also been associated with abnormal fetal growth, in subsequent pregnancies (Young et al 1998, Lazzari et al 2002, Farin et al 2004). However, these latter studies supplemented FBS up to 10%, while the current study utilized 2.5%.…”

Section: Immunofluorescene Of H3r26me2 During In Vitro Developmentmentioning

confidence: 99%

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

et al. 2015

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In vitro production (IVP) of bovine embryos is not only of great economic importance to the cattle industry, but is also an important model for studying embryo development. The aim of this study was to evaluate the histone modification, H3R26me2 during pre-implantation development of IVP bovine embryos cultured with or without serum supplementation and how these in vitro treatments compared to in vivo embryos at the morula stage. After in vitro maturation and fertilization, bovine embryos were cultured with either 0 or 2.5% fetal bovine serum (FBS). Development was evaluated and embryos were collected and fixed at different stages during development (2-, 4-, 8-, 16-cell, morula and blastocyst). Fixed embryos were then used for immunofluorescence utilizing an antibody for H3R26me2. Images of stained embryos were analyzed as a percentage of total DNA. Embryos cultured with 2.5% FBS developed to blastocysts at a greater rate than 0%FBS groups (34.85±5.43% vs. 23.38±2.93%; P<0.05). Levels of H3R26me2 changed for both groups over development. In the 0%FBS group, the greatest amount of H3R26me2 staining was at the 4-cell (P<0.05), 16-cell (P<0.05) and morula (P<0.05) stages. In the 2.5%FBS group, only 4-cell stage embryos were significantly higher than all other stages (P<0.01). Morula stage in vivo embryos had similar levels as the 0%FBS group, and both were significantly higher than the 2.5%FBS group. These results suggest that the histone modification H3R26me2 is regulated during development of pre-implantation bovine embryos, and that culture conditions greatly alter this regulation.

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Section: Immunofluorescene Of H3r26me2 During In Vitro Developmentmentioning

confidence: 99%

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

et al. 2015

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“…However, little is known about the possible effect of embryo cryopreservation and transfer on postnatal growth development in mammals. Only some insights into the roles of embryo manipulation on overgrowth syndrome in ruminants [23][24][25] and on growth traits in rabbits during the first 63 days of life [26] have been reported.…”

Section: Introductionmentioning

confidence: 99%

Embryo vitrification in rabbits: Consequences for progeny growth

et al. 2015

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The objective of this research is to examine if there are any effects of the rederivation procedures on rabbit growth pattern and on weight of different organ in adults. For this purpose, three experiments were conducted on two different groups of animals (control group and vitrified-transferred group) to evaluate the possible effect of embryo manipulation (vitrification and transfer procedures) on future growth traits. The first experiment studies body weight from 1 to 9 weeks of age from the two groups. The second experiment describes the growth curve of progeny from experimental groups and analyzes their Gompertz curve parameters, including the estimation of adult body weight. The third experiment has been developed to study if there are any differences in different organ weight in adult males from the two experimental groups. In general, the results indicate that rederivation procedures had effect on the phenotypic expression of growth traits. The results showed that rabbit produced by vitrification and embryo transfer had higher body weight in the first four weeks of age than control progeny. Results from body weight (a parameter) and b parameter estimated by fitting the Gompertz growth curve did not show any difference between experimental groups. However, differences related with growth velocity (k parameter of the Gompertz curve) were observed among them, showing that the control group had higher growth velocity than the vitrified-transferred group. In addition, we found that liver weight at 40th week of age exhibits significant differences between the experimental groups. The liver weight was higher in the control males than in the VF males. Although the present results indicate that vitrification and transfer pro-cedures might affect some traits related with growth in rabbits, further research is needed to assess the mechanisms involved in the appearance of these phenotypes and if these phenotypes could be transferred to the future progeny.

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“…O maior peso observado nos produtos de PIVE reforça evidências anteriores em bezerros taurinos de diferentes raças (Hasler et al 1995, Jacobsen et al 2000, Numabe et al 2000, Bertolini & Anderson 2002, mas contradiz outras (Jacobsen et al 2003, Rérat et al 2005. Definitivamente, não caracteriza, nos animais estudados, a ocorrência da condição denominada de Síndrome do Recém-nascido Grande (Large Offspring Syndrome), a qual é relatada em bezerros concebidos por métodos artificias in vitro (Young et al 1998, Farin et al 2006. Gasparelli et al (2009), acompanhando o nascimento de 122 bezerros Nelore produtos de PIVE, chegaram a observar dois bezerros nascidos com peso entre 50 e 59 kg, e três com peso superior a 60 kg.…”

Section: Resultsunclassified

“…No caso dos bezerros clonados já está bem caracterizado que nascem com tamanho e peso maiores, com vasos umbilicais de maior diâmetro e apresentam maior chance de desenvolverem doenças umbilicais (Garry et al 1996, Batchelder et al 2007, Miglino et al 2007, Meirelles et al 2010. A ocorrência da síndrome do bezerro grande (Large Offspring Syndrome) é comumente descrita em animais frutos de processos artificiais de fertilização e não somente nos bezerros clonados (Behboodi et al 1995, Kruip & den Daas 1997, Young et al 1998, Jacobsen et al 2000, Bertolini & Anderson 2002, Farin et al 2006. A maior susceptibilidade dos bezerros produtos de PIVE à doença umbilical é um assunto que merece ser melhor esclarecido.…”

Section: Introductionunclassified

Avaliação ultrassonográfica da involução das estruturas umbilicais extra e intracavitárias em bezerros sadios da raça Nelore concebidos naturalmente e produtos de fertilização in vitro

Sturion

Lisbôa

2013

Pesq. Vet. Bras.

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Esse trabalho foi desenvolvido com o objetivo de caracterizar a involução das estruturas umbilicais em bezerros sadios da raça Nelore ao longo dos primeiros 35 dias de vida, e de comparar esse processo em bezerros concebidos por métodos naturais ou por fertilização in vitro (FIV). Quarenta bezerros foram distribuídos em dois grupos (n=20) de acordo com o método de concepção (natural ou FIV) e cada grupo foi composto por dez machos e dez fêmeas. A ultrassonografia (transdutor microconvexo de 7,5 MHz) foi empregada para examinar o conjunto das estruturas remanescentes do cordão umbilical que compõem o umbigo externo e as estruturas abdominais (veia umbilical, artéria umbilical esquerda e ducto alantóide), mensurando-se os seus diâmetros em locais definidos. Os exames foram realizados entre 24 e 36 horas de vida e aos 7, 14, 21, 28 e 35 dias de idade. Testaram-se os efeitos do sexo, da idade e do método de concepção por meio da análise de variâncias de medidas repetidas. O exame ultrassonográfico provou-se adequado para a avaliação das estruturas umbilicais extra e intracavitárias permitindo a caracterização do processo fisiológico de involução das mesmas. No umbigo externo, as veias umbilicais foram observadas como imagem individualizada até os 14 dias de vida e um conjunto de estruturas em processo de atrofia era visualizado após essa idade. No abdômen, a veia e a artéria umbilicais foram visualizadas até os 35 dias de idade e o ducto alantóide somente durante a primeira semana de vida. Essas estruturas apresentaram-se com parede hiperecóica regular e contínua e lúmen homogeneamente anecóico. O diâmetro de todas as estruturas umbilicais estudadas se reduziu continuamente ao longo do primeiro mês de vida (p<0,05), sem efeito do sexo (p>0,05). Comparados aos bezerros concebidos por métodos naturais, os produtos de FIV nasceram com os vasos umbilicais e o ducto alantóide um pouco mais calibrosos (diâmetros 1 a 3 mm maiores). Distintamente dos valores mais elevados estabelecidos em estudos prévios para os bezerros de raças européias, pode-se admitir, por fim, que nos bezerros recém-nascidos sadios da raça Nelore a espessura das estruturas que compõem o umbigo externo não deve ultrapassar 2 cm, o diâmetro da veia e da artéria umbilicais pode chegar a 1 cm e o do ducto alantóide é próximo a 0,5 cm.

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Large offspring syndrome in cattle and sheep

Cited by 785 publications

References 16 publications

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

Embryo vitrification in rabbits: Consequences for progeny growth

Avaliação ultrassonográfica da involução das estruturas umbilicais extra e intracavitárias em bezerros sadios da raça Nelore concebidos naturalmente e produtos de fertilização in vitro

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