Langmuir-Blodgett films of immunoglobulines IgG. Ellipsometric study of the deposition process and of immunological activity

Layer-by-Layer Method for Immobilization of Protein Molecules on Biochip Surface

“…It has allowed us to store plated without special care before use. The SPR signal shift to the higher angle was observed by the subsequent treatment of gold surface with I mM thioacids or CystEst derivative for 12 hours. II-UDA provided most reproducible results among thioacids studied.…”

“…The surface has been modified by various methods, including Langmuir-Blodgett transfer [11][12][13][14], covalent binding [15][16][17][18], and spontaneous adsorption from solution [19][20][21] J.…”

A Fluorescent-Photochrome Method for the Quantitative Characterization of Solid Phase Antibody Orientation

“…Of the three immobilized systems, the LB method for solid phase immobilization of antibodies has been least investigated, although it has been shown that at a pressure of 20 mN/m and above, antibodies are densely packed and oriented in a random up-down manner at the gas-water interface (21,39). Physical adsorption of antibodies on glass or quartz gives rise to low surface densities, antibody orientation is casual, and the adsorption may be reversible in the presence of detergents or chaotropic agents (20).…”

Engineering of Enzyme Monolayer for Industrial Biocatalysis: An Overviewa

“…The resulting monolayer is then transferred onto the chosen substrate‐the Langmuir‐Schaefer method. As several problems may indeed occur in different classes of protein films, 1,3 like random orientation, denaturation, and protein movement, a systematic study 4–27 was carried out. Several biophysical techniques and a wide class of proteins, of different size, composition, and function, were utilized in order to identify the numerous parameters controlling the monolayer formation and to optimize each single existing problem (Table 1).…”

“…10 In this overview, the recent development in the engineering of enzyme monolayers that are more relevant to industrial biocatalysis 13–15 is summarized, with emphasis on the recycling stability and the cost‐effectiveness, along with the outline of a possible emerging bioreactor and the attempt to standardize an optimal procedure that takes into consideration the numerous parameters controlling the structure and the function of enzymes during thin film formation at the air/water interface. 16–27…”