Lactobacillus paracasei subsp. paracasei LC01 positively modulates intestinal microflora in healthy young adults

Abstract

Lactobacillus paracasei subsp. paracasei LC01 (LC01) can tolerate intestinal stresses and has antioxidant activity. To evaluate the effect of the bacterium on human intestinal microflora, a randomized, double-blind, placebo-controlled human trial was carried out. Fifty-two healthy adult volunteers were randomized equally to two groups. One group consumed 12% (wt/vol) skimmed milk supplemented with 10(10) CFU of LC01 each day for the 4-week treatment period, and then consumed placebo in the next treatment period, separated by a 2-week washout. The other group followed the reverse order. Group-specific real-time PCR and biochemical analyses was used to determine the intestinal bacterial composition of fecal samples collected at the end of every period, and the concentration of short-chain fatty acids and ammonia. A significant inhibition in fecal Escherichia coli and increase in Lactobacillus, Bifidobacterium, and Roseburia intestinalis were observed after consumption of LC01. Acetic acid and butyric acid were significantly higher in the probiotic stage and fecal ammonia was significantly lower. The results indicated a modulation effect of LC01 on the intestinal microflora of young adults, suggesting a beneficial effect on bowel health. LC01 may have potential value as a probiotic.

Effects of Regular Kefir Consumption on Gut Microbiota in Patients with Metabolic Syndrome: A Parallel-Group, Randomized, Controlled Study

“…Ameliorating the intestinal dysbiosis with prebiotics and probiotics have gained considerable attention in recent years for the management of MetS [45]. However, the studies have yielded inconsistent results regarding the influence of probiotics on fecal microbial diversity and composition [46,47,48,49,50,51,52]. Furthermore, the effects of kefir as a probiotic on gut microbiota have been examined very limited and mainly with animal studies.…”

Microbiota and Lifestyle: A Special Focus on Diet

“…Regarding changes in the levels of bacteria taxonomically related to the ingested probiotic strains, the most common finding in the reviewed studies is the increase in the fecal Lactobacillus [ 39 , 45 , 47 , 48 , 49 , 50 , 54 ] or Bifidobacterium levels [ 41 , 49 , 50 ], particularly after the intake of Lactobacillus strains, suggesting the existence of bacterial cross-feeding mechanisms in the intestinal niche. In this respect, there is evidence that some Lactobacillus strains can either degrade dietary carbohydrates or host mucin polysaccharides.…”

Antiviral effects of Lactobacillus ruminis SPM0211 and Bifidobacterium longum SPM1205 and SPM1206 on rotavirus-infected Caco-2 cells and a neonatal mouse model

“…Probiotics, such as Lactobacillus spp. and Bifidobacterium spp., are reported multiple potential health benefits including blocking gastroenteric pathogens, enhancing immune responses, and neutralizing viruses (Rolfe, 2000;Minocha, 2009;Zhang et al, 2013). Preidis et al (2011) and Sur et al (2011) reported that probiotics such as Lactobacillus rhamnosus, L. casei, Bifidobacterium bifidum, and Streptococcus thermophilus promote the innate immune response and stimulate cellular multiplication of epithelial cells.…”