Lactobacillus fermentum FTDC 8312 combats hypercholesterolemia via alteration of gut microbiota

Lye, Huey-Shi; Kato, Tamotsu; Low, Wai Yee; Taylor, Todd D.; Prakash, Tulika; Lew, Lee-Ching; Ohno, Hiroshi; Liong, Min‐Tze

doi:10.1016/j.jbiotec.2017.09.007

Cited by 48 publications

(31 citation statements)

References 33 publications

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“…These negatively correlated bacteria may play a role in the reduction of C. difficile numbers in the feces caused by probiotic treatment. Consistent with our results, Lactobacillus fermentum could alter the gut microbiota structure by increasing the relative abundance of Oscillospira (Lye et al, 2017) and oral probiotic cocktail of Lactobacillus and Bifidobacterium increased the level of Ruminococcus in feces after 8 weeks (De Wolfe et al, 2018). Besides, Coprococcus and Dorea were also reported to be lower level of abundance in C. difficile-infected patient (Shankar et al, 2014;Sokol et al, 2018).…”

Section: Discussionsupporting

confidence: 89%

Consortium of Probiotics Attenuates Colonization of Clostridioides difficile

Chu

Huang

et al. 2019

Front. Microbiol.

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Clostridioides difficile infection (CDI) is increasing morbidity and mortality rates globally. Fecal microbiota transplantation (FMT), an effective therapy for eliminating Clostridioides difficile (C. difficile), cannot be used extensive due to a range of challenges. Probiotics thus constitutes a promising alternative therapy. In our study, we evaluated the effect of consortium of probiotics including five Lactobacilli strains and two Bifidobacterium strains on the colonization of toxigenic BI/NAP1/027 C. difficile in a mouse model. The results of 16S rRNA sequencing and targeted metabolomics showed the consortium of probiotics effectively decreased the colonization of C. difficile, changed the αand β-diversity of the gut microbiota, decreased the primary bile acids, and increased the secondary bile acids. Spearman's correlation showed that some of the OTUs such as Akkermansia, Bacteroides, Blautia et al. were positively correlated with C. difficile numbers and the primary bile acids, and negatively correlated with the secondary bile acids. However, some of the OTUs, such as Butyricicoccus, Ruminococcus, and Rikenellaceae, were negatively correlated with C. difficile copies and the primary bile acids, and positively correlated with the secondary bile acids. In summary, the consortium of probiotics effectively decreases the colonization of C. difficile, probably via alteration of gut microbiota and bile acids. Our probiotics mixture thus offers a promising FMT alternative.

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Section: Discussionsupporting

confidence: 89%

Consortium of Probiotics Attenuates Colonization of Clostridioides difficile

Chu

Huang

et al. 2019

Front. Microbiol.

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“…Similarly, Lye et al. () determined that adding 1 × 10 9 CFU/ml of L. fermentarum FTDC 8313 increased Lactobacillus and Oscillospira counts in the fecal microbiota in BALB/c mice fed a high‐fat diet. Shi et al.…”

Section: Discussionmentioning

confidence: 93%

The effects of the supplementation of multi‐strain probiotics on intestinal microbiota, metabolites and inflammation of young SPF chickens challenged with Salmonella enterica subsp. enterica

Chang

Teng

Lee

et al. 2019

Animal Science Journal

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This study assessed the effect of probiotics on cecal microbiota, cecal short‐chain fatty acids (SCFAs), and the gene expression of cytokines in young specific‐pathogen‐free (SPF) chickens infected with S. enterica subsp. enterica. One‐day‐old SPF chickens (n = 105) were randomly assigned to one of the three treatment groups: control (Cont) group, Salmonella‐infected (Sal) group, and a Salmonella‐infected group treated with multi‐strain probiotics (ProSal group). All chickens except those in the Cont group were challenged orally with 1 × 108 cfu/ml of Salmonella 4 days after hatching. Chickens in the Sal group exhibited more abundance of Proteobacteria than those in the Cont and ProSal groups. At the genus level, chickens in ProSal group exhibited increased numbers of Lactobacillus and Oscillospira compared with those in the other groups. Chickens in the ProSal group exhibited a significant increase of cecal SCFAs compared with chickens in the Sal group. Chickens in the ProSal group exhibited increased gene expression of anti‐inflammatory cytokines, IL‐10 and TGF‐β4, and decreased expression of the proinflammatory cytokine, IFN‐γ, in the cecal tonsil compared with those in the Sal group. The results of this study indicated that the administration of probiotics can modulate microbiota, SCFAs, and immunomodulatory activity in SPF chickens.

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“…The disrupted micelles could not transport fatty acids to the enterocytes for absorption, leading to reduced cholesterol levels in serum. It was also found that bound-cholesterol are less likely to be absorbed into the small intestine and eventually resulted in an increased excretion of faecal cholesterol and reduced serum and liver cholesterol in rats (Lye et al 2010(Lye et al , 2017.…”

Section: Discussionmentioning

confidence: 99%

“…The homogenate was centrifuged at 5000 g for 2 min. The supernatant was collected to analyse for faecal TC, TG and TBA using Cholesterol Fluorometric Assay kit (Cayman), Triglyceride Colorimetric Assay kit (Cayman) and EnzyFluo TM Bile Acid Assay kit (BioAssay Systems), respectively (Lye et al 2017).…”

Section: Measurement Of Faecal Tc Tg and Tbamentioning

confidence: 99%

Cholesterol homeostasis associated with probiotic supplementation in vivo

Lim

Loke

et al. 2020

J. Appl. Microbiol.

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Aims: To determine the mechanism underlying the serum cholesterol reduction effect by probiotics isolated from local fermented tapioca (Tapai). Methods and Results: Lactic acid bacteria strains were isolated and examined for acid tolerance, bile salt resistance and hypocholesterolemic properties. Among the isolates, Lactobacillus plantarum TAR4 showed the highest cholesterol reduction ability (48Á01%). The focus in the in vivo trial was to elucidate the cholesterol balance from findings pertaining to serum cholesterol reduction in rat model fed with high fat diet via oral administration. Rats fed with high-cholesterol diet supplemented with Lact. plantarum TAR4 showed significant reduction in serum total cholesterol (29Á55%), serum triglyceride (45Á31%) and liver triglyceride (23Á44%) as compared to high-cholesterol diet (HCD) group. There was a significant increment in faecal triglyceride (45Á83%) and faecal total bile acid (384Á95%) as compared to HCD group. Conclusions: The findings showed that probiotic Lact. plantarum TAR4 supplementation reduced the absorption of bile acids for enterohepatic recycling and increased the catabolism of cholesterol to bile acids and not by suppressing the rate of cholesterol synthesis. Significance and Impact of Study: Probiotic supplements could provide a new nonpharmacological alternative to reduce cardiovascular risk factors. Key Points • Lactobacillus plantarum TAR4 exhibited an excellent tolerance to acid and bile salts. • Lactobacillus plantarum TAR4 was able to assimilate cholesterol in vitro. • Lactobacillus plantarum TAR4 had the ability to regulate cholesterol metabolism levels in rats. • Hypocholesterolemic effect related to cholesterol binding and deconjugation of bile acid by Lactobacillus plantarum TAR4.

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Lactobacillus fermentum FTDC 8312 combats hypercholesterolemia via alteration of gut microbiota

Cited by 48 publications

References 33 publications

Consortium of Probiotics Attenuates Colonization of Clostridioides difficile

Consortium of Probiotics Attenuates Colonization of Clostridioides difficile

The effects of the supplementation of multi‐strain probiotics on intestinal microbiota, metabolites and inflammation of young SPF chickens challenged with Salmonella enterica subsp. enterica

Cholesterol homeostasis associated with probiotic supplementation in vivo

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