Lactobacillus exopolysaccharides: New perspectives on engineering strategies, physiochemical functions, and immunomodulatory effects on host health

Rajoka, Muhammad Shahid Riaz; Wu, Yu-Fei; Mehwish, Hafiza Mahreen; Bansal, Manisha; Zhao, Lingyun

doi:10.1016/j.tifs.2020.06.003

Cited by 103 publications

(53 citation statements)

References 156 publications

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“…Высокие энергозатраты при биосинтезе полимеров указывают на то, что эти соединения играют важную роль в жизнедеятельности бифидобактерий. Предполагается, что ЭПС играет роль барьера, защищая микроорганизмы от агрессивных факторов окружающей среды, в том числе от антибиотиков, факторов иммунитета, секретов желудочно-кишечного тракта [3,18,19,20]. Так, кислотоустойчивость производственного штамма B. breve BB8 связана с увеличенной продукцией ЭПС [21].…”

Section: биологическая роль эпс бифидобактерийunclassified

“…В настоящее время микробные экзополисахариды (ЭПС) стали привлекать внимание ученых благодаря их большому медицинскому, фармацевтическому и промышленному потенциалу. Поиск штаммов, продуцирующих ЭПС, особенно активно осуществляется для производства ферментированных молочных продуктов, так как эти полимеры положительно влияют на вязкость, текстуру готовой продукции и повышают ее биологическую ценность [2,3]. В связи с этим функциональная активность, биологические эффекты, условия их продукции наиболее подробно описаны у молочнокислых микроорганизмов, в частности у представителей рода Lactobacillus [3,4].…”

Section: Introductionunclassified

“…Поиск штаммов, продуцирующих ЭПС, особенно активно осуществляется для производства ферментированных молочных продуктов, так как эти полимеры положительно влияют на вязкость, текстуру готовой продукции и повышают ее биологическую ценность [2,3]. В связи с этим функциональная активность, биологические эффекты, условия их продукции наиболее подробно описаны у молочнокислых микроорганизмов, в частности у представителей рода Lactobacillus [3,4]. Однако исследования механизмов взаимодействия микробиоты в многокомпонентных сообществах показали, что продукция ЭПС является широко распространенным фенотипическим признаком у многих ми- [5,6].…”

Section: Introductionunclassified

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Bifdobacterial exopolysaccharides: a brief review

Захарова¹,

Леванова²,

Отдушкина³

2021

Fundamentalʹnaâ i kliničeskaâ medicina

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Exopolysaccharide (EPS) production is a widespread phenotypic trait in many commensal and pathogenic microorganisms. In bifidobacteria, the discovery of the eps gene cluster propelled the multiple studies of their EPS, which represent heteropolysaccharides and generally consist of three monosaccharides: D-glucose, D-galactose, and L-rhamnose. EPS of B. animalis subsp. lactis additionally contains mannose while EPS of B. adolescentis and B. longum subsp. longum contains 6-deoxytealose. The number of repeat units in bifidobacterial EPS is a straincharacteristic feature. Precursors of the indicated EPS monomers are glucose-1-phosphate and fructose-6-phosphate, and the synthesis involves nucleotide sugar intermediates. Two molecular systems are implica in polymerisation and polymer secretion in bifidobacteria: ABC transporters and fippase polymerase complex (Wzx/Wzydependent pathway). EPS perform numerous functions. They protect bifidobacteria from aggressive gastrointestinal milieu and reactive oxygen species, provide a scaffold for the bacterial-bacterial interactions, and act as the receptors for phage adsorption. Further, EPS are used by the other members of the gut microbiota as substrates for nutrition, i.e. bifidobacteria regulate the composition and metabolic activity of intestinal microorganisms. Therefore, EPS-producing strains exhibit pronounced antibacterial effects due to the binding of opportunistic and pathogenic microbes. Finally, EPS can act as pathogen-associated molecular patterns. Beneficial effects of bifidobacterial EPS determined the possibility of their use as prebiotics or as a part of symbiotics. The main limitation in this regard is the low yield of the target product when culturing EPS-producing strains. Therefore, current research is aimed at finding novel EPSproducing strains among the bifidobacteria and creating favorable technological conditions that promote EPS production.

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Section: биологическая роль эпс бифидобактерийunclassified

Section: Introductionunclassified

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Bifdobacterial exopolysaccharides: a brief review

Захарова¹,

Леванова²,

Отдушкина³

2021

Fundamentalʹnaâ i kliničeskaâ medicina

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“…LAB synthesize two types of EPSs including homopolysaccharides and heteropolysaccharides [17] . Homopolysaccharide synthesis is a relatively simple biochemical process involving a specific GT (glucansucrase or fructansucrase) and an extracellular sugar donor (sucrose for the synthesis of glucans, but it can also be other fructose-containing oligosaccharide (e.g.…”

Section: Epss Biosynthesis In Labmentioning

confidence: 99%

“…In recent decades, the immunomodulatory potential of EPSs has received a lots of scientific consideration. Many in-vitro studies have demontrated that EPSs produced by different LAB species have the immunomodulatory ability [17] . The phosphate group (a good inducer of the immune response) plays a critical role and characterizes the immunomodulatory effects of EPSs.…”

Section: The Physiological Functions Of Epssmentioning

confidence: 99%

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

Nguyen¹,

Nguyen²,

Bui³

et al. 2020

AIMS Microbiology

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Exopolysaccharides (EPSs) are biological polymers secreted by microorganisms including Lactic acid bacteria (LAB) to cope with harsh environmental conditions. EPSs are one of the main components involved in the formation of extracellular biofilm matrix to protect microorganisms from adverse factors such as temperature, pH, antibiotics, host immune defenses, etc.. In this review, we discuss EPS biosynthesis; the role of EPSs in LAB stress tolerance; the impact of environmental stresses on EPS production and on the expression of genes involved in EPS synthesis. The evaluation results indicated that environmental stresses can alter EPS biosynthesis in LAB. For further studies, environmental stresses may be used to generate a new EPS type with high biological activity for industrial applications.

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