Lactobacillus crispatus KT-11 Enhances Intestinal Immune Functions in C3H/HeN Mice

Abstract

SummaryWe investigated the effect of Lactobacillus crispatus KT-11 (KT-11) on intestinal immune systems in C3H/HeN mice. The level of intestinal total immunoglobulin (Ig) A was significantly higher in mice given KT-11 than in mice not given KT-11. Gene expression relating to antibody production and innate immune response increased more than 2-fold in the former compared with the later. Moreover, the number of IL-6 ϩ CD11bϩ cells was significantly higher in Peyer's patch cells cultured with KT-11 than in those cultured without KT-11, although the number of CD4 ϩ cells and the cell ratio of CD4 ϩ /CD8 ϩ were remarkably lower in the culture with KT-11. These results indicate that KT-11 enhances intestinal IgA production and innate immune response in C3H/HeN mice.

Enhancement of Microbiota in Healthy Macaques Results in Beneficial Modulation of Mucosal and Systemic Immune Function

“…Indeed, previous work has shown that treatment of mice with various strains of PBio lactobacilli spp. induced an increase in the level of IgA in the intestine (28, 29) and an increase in the frequency of small intestine IgA-expressing B cells (30). In order to assess whether PBio treatment with VSL#3 resulted in commensurate changes to Ig phenotype, we measured the frequency of B cells that expressed IgA versus IgG before and after PBio therapy using flow cytometry (Figure 2A, representative staining).…”

Cross-talk between probiotic lactobacilli and host immune system

“…Lactobacillus crispatus K‐11 increased mucin 13 and defensin alpha genes expression in Peyer's patches by twofold compared with control, ensuring constant protection against the attack of digestive fluid, micro‐organisms, pollutants and toxins (Tobita et al . ). Moreover, the pili‐encoding operon spaABC of Lact.…”

“…crispatus K‐11 did not influence the Th1/Th2 balance as illustrated by no change in the IFN‐ γ + CD4 + and IL‐4 + CD4 + cells in the normal C#H/HeN mice (Tobita et al . ). However, it reduced allergic symptoms by shifting the Th1/Th2 balance from Th2 dominant state to a Th1 dominant state in type I allergic model mice (Tobita et al .…”

Discrimination of the Lactobacillus acidophilus group using sequencing, species‐specific PCR and SNaPshot mini‐sequencing technology based on the recA gene

“…bulgaricus species have been shown to be probiotic and are currently widely used in the food industry 2. Moreover, numerous studies have demonstrated that these probiotic Lactobacillus strains provide beneficial effects to the human immune system 3–5…”