Lactic acid bacterium, Lactobacillus paracasei KW3110, suppresses inflammatory stress-induced caspase-1 activation by promoting interleukin-10 production in mouse and human immune cells

Yamazaki, Takahiro; Ohshio, Konomi; Sugamata, Miho; Morita, Yuji

doi:10.1371/journal.pone.0237754

Cited by 5 publications

(6 citation statements)

References 32 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous studies have shown that KW3110 ameliorates the inflammatory responses of macrophages through promoting anti-inflammatory cytokine IL-10 production [17,18]. In this study, we investigated whether IL-10 suppresses inflammatory-stress-induced mitochondrial dysfunction in J774A.1 cells.…”

Section: Il-10 Suppresses Mitochondrial Dysfunction In Inflammatory-s...mentioning

confidence: 94%

“…Mitochondrial ROS production was analyzed in cells labeled with MitoTracker Green and MitoSOX and the ratio of cells with higher MitoSOX intensity than MitoTracker Green intensity (SOX high cells) to total cells was calculated. While cells were treated with 10 µg/mL LPS for 4 h followed by treatment with ATP for 2 h aiming to prime and activate IL-1β in our previous study [17,19], in this study, we used a lower concentration of LPS alone to induce mitochondrial damage in accordance with the experimental condition of another group's study [38,39]. Additionally, the concentration of KW3110 was lower in this study than that used in our previous study because the LPS concentration used in this study was low and KW3110 treatment (5 µg/mL and lower) with J774A.1 cells significantly induced IL-10 production (Figure 4A).…”

Section: Mitochondrial Membrane Potential and Ros Analyses By Flow Cy...mentioning

confidence: 99%

“…Our previous reports have shown that a specific strain of LAB, Lactobacillus paracasei KW3110 (KW3110), has antiallergy and anti-inflammatory effects in mice and humans [12,[14][15][16][17][18][19]. In addition, administration of whole bacteria of KW3110 prevented ageing-related chronic inflammation and blue light exposure induced retinal inflammation in mice [20,21].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lactobacillus paracasei KW3110 Prevents Inflammatory-Stress-Induced Mitochondrial Dysfunction in Mouse Macrophages

Yamada

Ohshio

Sugamata

et al. 2022

IJMS

Self Cite

View full text Add to dashboard

Lactobacillus paracasei KW3110 (KW3110) has anti-inflammatory effects, including the prevention of blue light exposure induced retinal inflammation and ageing-related chronic inflammation in mice. The mechanism involves the promotion of anti-inflammatory cytokine interleukin (IL)-10 production by KW3110, leading to reduced pro-inflammatory cytokine IL-1β production. Although various stress-induced mitochondrial damages are associated with excessive inflammatory responses, the effect of KW3110 on inflammatory-stress-induced mitochondrial damage remains unknown. In this study, we investigated the effect of KW3110 on inflammatory stress-induced mitochondrial damage using the murine macrophage-like cell line J774A.1. KW3110 treatment suppressed lipopolysaccharide (LPS)-induced mitochondrial dysfunction, including downregulation of membrane potential, induction of reactive oxygen species, and respiratory dysfunction. In addition, KW3110 prevented LPS-induced disruption of mitochondrial morphology including cristae structures. IL-10 treatment also ameliorated LPS-induced mitochondrial dysfunction and morphology disruption. These results suggest that KW3110 prevents LPS-induced mitochondrial dysfunction, potentially via promoting IL-10 production in mouse macrophages. We are the first to reveal a suppressive effect of lactic acid bacteria on mitochondrial morphology disruption in inflammatory-stressed macrophages. Our findings contribute to understanding inflammatory-stress-induced mitochondrial damage and developing food ingredients with preventive effects on mitochondrial-damage-derived inflammatory conditions.

show abstract

Section: Il-10 Suppresses Mitochondrial Dysfunction In Inflammatory-s...mentioning

confidence: 94%

Section: Mitochondrial Membrane Potential and Ros Analyses By Flow Cy...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lactobacillus paracasei KW3110 Prevents Inflammatory-Stress-Induced Mitochondrial Dysfunction in Mouse Macrophages

Yamada

Ohshio

Sugamata

et al. 2022

IJMS

Self Cite

View full text Add to dashboard

show abstract

“…Treatment with probiotic strain L. acidophilus DDS-1 upsurges the abundance of beneficial bacteria such as Lactobacillus spp and Akkermansia spp and also the levels of butyrate, while downregulating the production of inflammatory cytokines IL-6, IL-1β, IL-1α, MCP-1, Macrophage Inflammatory Protein (MIP)-1α, MIP-1β, IL-12 and IFN-γ in aging mice 101 . L. paracasei KW3110 suppresses hyperinflammation via activation of M2 macrophages and exhibit antiinflammatory effects via suppression of IL-β production and caspase 1 activation by promoting IL-10 production 103 104,114 . Use of probiotics may restore the healthy gut microbiome in CoVID-19 patients and exhibit antiviral effects through gut-lung axis.…”

Section: Probiotics: Suppressors Of Respiratory Tract Infections and Inflammationmentioning

confidence: 99%

“…Treatment with probiotic strain L. acidophilus DDS-1 upsurges the abundance of beneficial bacteria such as Lactobacillus spp and Akkermansia spp and also the levels of butyrate, while downregulating the production of inflammatory cytokines IL-6, IL-1β, IL-1α, MCP-1, Macrophage Inflammatory Protein (MIP)-1α, MIP-1β, IL-12 and IFN-γ in aging mice 101 . L. paracasei KW3110 suppresses hyperinflammation via activation of M2 macrophages and exhibit anti-inflammatory effects via suppression of IL-β production and caspase 1 activation by promoting IL-10 production 103 . Probiotic complex of L. acidophilus, L. casei, L. fermentum, L. paracasei, Streptococcus thermophilus, Bifidobacterium longum, B. bifidum, B. breve, L. rhamnosus, L. plantarum, L. helveticus, and L. salivarius in combination with zinc and coenzyme Q10 can improve autoimmune arthritis via downregulation of proinflammatory cytokines including IL-6, IL-17 and TNF-α and inhibition of T-helper cell 17 (Th17) cell differentiation 109 – 111 .…”

Section: Probiotics: Suppressors Of Respiratory Tract Infections and Inflammationmentioning

confidence: 99%

Is butyrate a natural alternative to dexamethasone in the management of CoVID-19?

Patil

Bhandary³

et al. 2021

F1000Res

View full text Add to dashboard

Coronavirus disease 2019 (CoVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 has affected more than 100 million lives. Severe CoVID-19 infection may lead to acute respiratory distress syndrome and death of the patient, and is associated with hyperinflammation and cytokine storm. The broad spectrum immunosuppressant corticosteroid, dexamethasone, is being used to manage the cytokine storm and hyperinflammation in CoVID-19 patients. However, the extensive use of corticosteroids leads to serious adverse events and disruption of the gut-lung axis. Various micronutrients and probiotic supplementations are known to aid in the reduction of hyperinflammation and restoration of gut microbiota. The attenuation of the deleterious immune response and hyperinflammation could be mediated by short chain fatty acids produced by the gut microbiota. Butyric acid, the most extensively studied short chain fatty acid, is known for its anti-inflammatory properties. Additionally, butyric acid has been shown to ameliorate hyperinflammation and reduce oxidative stress in various pathologies, including respiratory viral infections. In this review, the potential anti-inflammatory effects of butyric acid that aid in cytokine storm depletion, and its usefulness in effective management of critical illness related to CoVID-19 have been discussed.

show abstract

Lactic acid bacterium, Lactobacillus paracasei KW3110, suppresses inflammatory stress-induced caspase-1 activation by promoting interleukin-10 production in mouse and human immune cells

Cited by 5 publications

References 32 publications

Lactobacillus paracasei KW3110 Prevents Inflammatory-Stress-Induced Mitochondrial Dysfunction in Mouse Macrophages

Lactobacillus paracasei KW3110 Prevents Inflammatory-Stress-Induced Mitochondrial Dysfunction in Mouse Macrophages

Is butyrate a natural alternative to dexamethasone in the management of CoVID-19?

Contact Info

Product

Resources

About