Lactic acid bacteria as antigen delivery vehicles for oral immunization purposes

Effect of Environmental Stresses on S-Layer Production in Lactobacillus acidophilus ATCC 4356

“…The degree of retention is likely dependent on the ability of the bacteria to interact with eukaryotic cell surfaces or with the mucosal layer surrounding these cells (Altermann et al, 2004). Several factors contribute to the interaction of Lactobacilli with the host tissues, such as cell surface hydrophobicity (Vadillo-Rodriguez et al, 2005;Van der Mei et al, 2003), autoaggregation (Kos et al, 2003), lipoteichoic acids (Granato et al, 1999) and external surface proteins (such as S-layer, fibronectin and mucinbinding proteins) (Altermann et al, 2004;Avall-Jaaskelainen & Palva, 2005;Frece et al, 2005;Kos et al, 2003;Kosin & Rakshit, 2006;Pouwels et al, 1998;Velez et al, 2007;Ventura et al, 2002). Surface layer (S-layer) has been identified as the outermost structure of cell envelope in numerous organisms from the domains Bacteria (in both Gram-positive and Gramnegative Eubacteria) and Archaea (Debabov, 2004;Sara & Sleytr, 2000).…”

“…Species of these genera can be found in gastrointestinal tract of man and animal Klein et al, 1998;Mathur & Singh, 2005). They have also been involved since time immemorial in food processing and food preservation, and are applied in particular for the manufacturing of dairy products, fermented meat, vegetables, bread and ensilage (Pouwels et al, 1998). Several lactic acid bacteria have the potential to promote the health of the host or to prevent and treat diseases.…”

Dendritic cell targeting ofBacillus anthracisprotective antigen expressed byLactobacillus acidophilusprotects mice from lethal challenge

“…Although many of the properties related to their pathogenicity make them attractive candidates for inducing immune responses, the potential for reversion of attenuated strains to virulence is a significant safety concern. Moreover, these bacteria are highly immunogenic, which may prevent their use in vaccine regimens requiring multiple doses (4).…”

Autohydrolysed Tilapia nilotica Fish Viscera as a Peptone Source in Bacteriocin Production

“…The promising results obtained with L. sakei are of interest because lactobacilli (and other LAB) have a GRAS (generally regarded as safe) status, because some LAB have potential as probiotics, and because some LAB may be exploited as (in situ) food-grade cell factories and delivery vehicles for e.g. antigens, antibodies and growth factors [20]. Thus the ability to effectively grow bacteria on media free of compounds derived from meat sources or from plant sources (e.g.…”