Lactic acid as a substrate for fermentative hydrogen production

Grause, Guido; Igarashi, Masashige; Kameda, Tomohito; Yoshioka, Toshiaki

doi:10.1016/j.ijhydene.2012.08.096

Cited by 34 publications

(18 citation statements)

References 26 publications

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“…In general, if HRT was in the range of 18.7 to 10 h, acetate consumption was higher (85.9%) than lactate. The presence of acetate promoted a higher biohydrogen production rate . In the absence of acetate, cells have first to provide acetate by some alternative metabolic processes.…”

Section: Resultsmentioning

confidence: 99%

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Continuous two-staged co-digestion process for biohydrogen production from agro-industrial wastes

Gómez-Romero

Gonzalez-Garcia

Chairez

et al. 2015

Int. J. Energy Res.

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SUMMARYMexico produces large amounts of organic residues. Twenty milliard tons of fruit and vegetable wastes (FVW) are produced yearly. On the other hand, the cheese processing industry produces crude cheese whey (CCW) with an annual production of 1 million metric tons. These types of residues are well characterized, and both constitute a potential feedstock for biohydrogen production, individually or as a mixture. Recently, we demonstrated the feasibility of utilizing CCW and FVW in a co-digestion process to produce biohydrogen. The possibility to design an effective process in a two stage configuration was evaluated based in our previous studies of the by-products and description of microbial ecology evolution of the co-digestion process. An initial assimilation of easily degradable carbohydrates and protein allowed cell growth and acetate and lactate as main byproducts. The co-digestion and two-stage processes increased several times the biohydrogen yield compared with those obtained from single substrate and in a co-digestion single stage process. Higher biohydrogen productivity was a result of reaction stability in comparison to single-substrate digestion and one-stage processes.The effect of hydraulic retention time (HRT) and organic loading rate (OLR) on the volumetric hydrogen production rate (VHPR) and yield (Y H2 ) was evaluated in a two-staged co-digestion process of FVW and CCW. The system consisted of hydrolytic and hydrogenic bioreactors operated in batch mode and continuous regime (89 days), respectively. Eight conditions of HRTs and respective OLRs were evaluated. As HRT decreased, the VHPR increased proportionally, reaching the highest average values around 7 L L À1 d À1 at an HRT between 15 and 17.5 h. The highest Y H2, (813.3 mL H 2 g COD À1 ) was determined at 17.5 h (OLR, 80.02 g COD L À1 d À1 ). Based on the distribution of the final products, the processes occur sequentially and reach a steady state set by the OLR. Biohydrogen production is a result of a syntrophic microbial activity. In general, the two stages of the co-digestion process provided higher stability, reliability, and efficiency.

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Section: Resultsmentioning

confidence: 99%

“…Two‐stage processes using lactate/glucose and ethanol as substrates have been reported recently . Previous studies agreed that co‐digestion and two‐stage processes could provide higher biohydrogen productivity and reaction stability in comparison to single‐substrate digestion and one‐stage processes .…”

Section: Introductionmentioning

confidence: 96%

Continuous two-staged co-digestion process for biohydrogen production from agro-industrial wastes

Gómez-Romero

Gonzalez-Garcia

Chairez

et al. 2015

Int. J. Energy Res.

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“…Lactobacillus spp. can utilize a wide range of sugars as the carbon source, while producing either mainly lactate (homofermentation) or lactate, acetate, and ethanol (heterofermentation), while one species within this genus is able to convert lactate to acetate and formate (and in rare cases form H2) [59]. Thus, the formation of acetate from lactate has been observed while fermenting food waste at regulated pH 7, with associated H2 production [60].…”

Section: Food Waste Reactorsmentioning

confidence: 99%

“…can utilize a wide range of sugars as the carbon source, while producing either mainly lactate (homofermentation) or lactate, acetate, and ethanol (heterofermentation), while one species within this genus is able to convert lactate to acetate and formate (and in rare cases form H 2 ) [58]. Thus, the formation of acetate from lactate has been observed while fermenting food waste at regulated pH 7, with associated H 2 production [59]. These three routes may, therefore, have occurred simultaneously to differing extents in the food waste reactors, explaining the divergent patterns of lactic and acetic acid production in the reactors (Figure 2).…”

Section: Food Waste Reactorsmentioning

confidence: 99%

Inoculum Source Determines Acetate and Lactate Production during Anaerobic Digestion of Sewage Sludge and Food Waste

et al. 2019

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Acetate production from food waste or sewage sludge was evaluated in four semi-continuous anaerobic digestion processes. To examine the importance of inoculum and substrate for acid production, two different inoculum sources (a wastewater treatment plant (WWTP) and a co-digestion plant treating food and industry waste) and two common substrates (sewage sludge and food waste) were used in process operations. The processes were evaluated with regard to the efficiency of hydrolysis, acidogenesis, acetogenesis, and methanogenesis and the microbial community structure was determined. Feeding sewage sludge led to mixed acid fermentation and low total acid yield, whereas feeding food waste resulted in the production of high acetate and lactate yields. Inoculum from WWTP with sewage sludge substrate resulted in maintained methane production, despite a low hydraulic retention time. For food waste, the process using inoculum from WWTP produced high levels of lactate (30 g/L) and acetate (10 g/L), while the process initiated with inoculum from the co-digestion plant had higher acetate (25 g/L) and lower lactate (15 g/L) levels. The microbial communities developed during acid production consisted of the major genera Lactobacillus (92-100%) with food waste substrate, and Roseburia (44-45%) and Fastidiosipila (16-36%) with sewage sludge substrate. Use of the outgoing material (hydrolysates) in a biogas production system resulted in a non-significant increase in bio-methane production (+5-20%) compared with direct biogas production from food waste and sewage sludge.by slower-growing bacteria that thrive best at neutral pH. These differences in reaction speed and pH optimum allow AD to be divided into two separate production steps, where H 2 and VFA are generated in a primary acid reactor and bio-methane in a secondary reactor. This two-stage approach has been shown to optimize the overall AD process and increase methane yield [3][4][5][6][7][8]. It also allows additional applications, such as the production of bio-hydrogen or of a range of VFA that can be extracted and used as "green" chemical feedstock for further conversion [8,9]. The typical VFA composition in an acidic AD reactor is a mixture dominated by acetate, propionate, butyrate, and valerate [10-13], but it can also be dominated by acetate, butyrate, and H 2 [14], or mainly consist of lactate and/or acetate [15,16]. The amount and type of acid produced depend on both chemical, technical, and microbiological parameters, often interlinked. Parameters shown to be of importance include, for example, reactor configuration, substrate composition and pre-treatment, hydraulic retention time (HRT), temperature, pH, as well as the choice of inoculum and final microbial composition [10,11,[17][18][19][20][21][22].Among the different acids that can be produced in a two-stage set-up, acetate is a highly interesting compound. In addition to being an energy carrier between different stages within AD, it can be used for the production of value-added products such as biopolymers, com...

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“…Embora seja um processo espontâneo e apresente maiores rendimentos entre os processos de bioprodução de H 2 , a fermentação ainda é sensível a diversos fatores operacionais, e assim o principal objetivo da maioria dos estudos de pesquisa relaciona-se a possibilidade de aumento no rendimento (PERERA et al, 2012). A questão seria focada em como atingir condições ideais de produção de hidrogênio, a partir de reduzidos custos de operação, considerando a configuração do reator, temperatura, concentração de substrato e taxa de carregamento orgânico aplicada orgânica aplicada (WANG e WAN, 2009;GRAUSE et al, 2012;GIOANNIS et al, 2013).…”

Section: Introductionunclassified

Produção de hidrogênio em reator anaeróbio de leito fluidificado termofílico com vinhaça como substrato orgânico

Santos¹

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Por me ensinarem, mais uma vez, os reais valores da vida. "Haja o que houver, distribua confiança e bom ânimo, porque a alegria é talvez a única dádiva que você é capaz de ofertar sem possuir." André Luiz por Chico Xavier AGRADECIMENTOS A Deus, pelo dom da vida, por mais esta oportunidade de crescimento pessoal e profissional e por colocar pessoas tão queridas e especiais para guiar o meu caminho. Ao Prof. Dr. Edson Luiz Silva pelo aceite de orientação, pelo incentivo, comprometimento, experiência e auxílio em todas as etapas até que este projeto se tornasse realidade. Agradeço-o pela dedicação, paciência e competência com que orientou este trabalho. A Prof a. Dr a. Maria Bernadete Amâncio Varesche pela recepção, conselhos, encorajamento e oportunidade de co-orientação deste projeto. Agradeço as valiosas contribuições durante estes três anos de convívio, o aprendizado diário durante o Programa de Aperfeiçoamento Estudantil e as fundamentais considerações no Exame de Qualificação.

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Lactic acid as a substrate for fermentative hydrogen production

Cited by 34 publications

References 26 publications

Continuous two-staged co-digestion process for biohydrogen production from agro-industrial wastes

Continuous two-staged co-digestion process for biohydrogen production from agro-industrial wastes

Inoculum Source Determines Acetate and Lactate Production during Anaerobic Digestion of Sewage Sludge and Food Waste

Produção de hidrogênio em reator anaeróbio de leito fluidificado termofílico com vinhaça como substrato orgânico

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