Lactate fraction dependent mechanical properties of semitransparent poly(lactate-co-3-hydroxybutyrate)s produced by control of lactyl-CoA monomer fluxes in recombinant Escherichia coli

Yamada, Miwa; Matsumoto, Ken’ichiro; Uramoto, Shu; Motohashi, Ren; Abe, Hideki; Taguchi, Seiichi

doi:10.1016/j.jbiotec.2011.05.011

Cited by 57 publications

(52 citation statements)

References 38 publications

(50 reference statements)

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“…Recently, LA-based polyester P(LA-co-3HB)s were shown to be flexible and semitransparent material depending on their LA fractions (Yamada et al, 2011). Because the MCL units with 8-12 carbons should have a more potent effect of material softening than 3HB units, the physical properties of P(GL-co-3HA) and P(LA-co-3HA) comprise an interesting research target.…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase

Matsumoto

Ishiyama

Sakai

et al. 2011

Journal of Biotechnology

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Glycolate(GL)-based polyesters were for the first time produced in the recombinant Escherichia coli fatty acid -oxidation pathway reinforcing mutant LS5218, using extracellularly added GL as a monomer precursor. Cells expressing a Ser325Thr/Gln481Lys mutant of polyhydroxyalkanoate synthase (PhaC1STQK) from Pseudomonas sp. 61-3, propionyl-CoA transferase from Megasphaera elsdenii and enoyl-CoA hydratase from Pseudomonas aeruginosa grown on GL and dodecanoate were found to produce novel copolymers of GL with 3-hydroxyalkanoates (3HAs) (C 4 -C 12 ), P(GL-co-3HA), with a weight-average molecular weight of 34 000. The 1 H NMR analysis of the copolymer revealed the incorporation of GL units into the polymer chain. This result demonstrates that PhaC1STQK polymerized glycolyl-CoA as a monomer substrate. Additionally, the novel lactate(LA)-based polyester P(LA-co-3HA) was produced by substituting GL with LA, indicating that the method is versatile and allows the production of a variety of biopolymers. Keywords:poly (glycolide) poly (lactide) lactate-polymerizing enzyme biobased plastic polyhydroxyalkanoate

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Section: Discussionmentioning

confidence: 99%

Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase

Matsumoto

Ishiyama

Sakai

et al. 2011

Journal of Biotechnology

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“…The monomeric constituents and their ratio affect polymer properties due to the altered crystallinity and melting temperatures (T m ) (25). Previously, it had been demonstrated that incorporation of 6 mol% of LA into P(LA-co-3HB) reduces the T m of the copolymer compared to that of P(3HB) (8).…”

Section: Production Of La-enriched Copolymersmentioning

confidence: 99%

“…The advantage of using of xylose for the production of P(LA-co-3HB) was justified by the higher cellular polymer content and LA fraction of the copolymer compared to the utilization of glucose. In earlier studies using glucose, copolymers with LA fraction greater than 50 mol% were characterized with low polymer yields (less than 1 g/L) from 20 g/L glucose, limiting exploration of polymer properties (25). However, when using xylose, 7.3 g/L of P(60 mol% LA-co-3HB) was obtained, demonstrating the dual advantage of using xylose (35).…”

Section: Xylose Use For the Production Of P(la-co-3hb)mentioning

confidence: 99%

“…It was found that the molecular weight of biosynthesized LA-based polymer has an inverse relationship with LA fraction (Table 1) (23,25). This may be due to the fact that although LPE has acquired the ability to polymerize LA unit, the LA-CoA was still not preferencially used as the substrate to synthsize high-molecular weight polymers by LPE.…”

Section: Sequential Structure and Molecular Weight Of La-based Polymersmentioning

confidence: 99%

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Biosynthesis, Properties, and Biodegradation of Lactate-Based Polymers

Nduko

Sun

Taguchi

2015

ACS Symposium Series

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Tel/Fax: +81-11-706-6610.The tight crude oil supply and its negative impacts on the environment have accelerated efforts towards the search for alternatives to the conventional petrochemical-derived plastics. Polylactic acid (PLA), a polymer produced from renewable resources has emerged as a potential substitute to the conventional synthetic petrochemical-derived plastics owing to their biodegradable and bioresorbable nature. Despite these advantages, PLA has had little success as a substitute to the conventional petrochemical-derived plastics because the PLA synthesis; a two-step bio-chemo process where fermentative lactic acid is polymerized using heavy metal catalysts is expensive. Furthermore, the heavy metal catalyst remnants in the polymer hinder the use of PLA for medical devices and food handling packages. To overcome these obstacles, bacteria have been genetically manipulated to synthesize lactate (LA)-based polymers in a single-step metal-free system. This chapter reviews the production of LA-based polymers using a lactate-polymerizing enzyme (LPE). Production of LA-enriched and PLA-like polymers in bacteria from glucose and xylose, metabolic/ genetic engineering for polymer yield enhancement, polymer properties, and the biodegradability of the LA-based polymers will be discussed. Moreover, future perspectives on the synthesis and applications of the LA-based polymers will be mentioned.

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“…P(LA-co-3HB)s are processed into a semitransparent film depending on the monomer composition. The copolymer with higher LA fraction tends to be more transparent [20]. As mentioned above, the transparency was determined by the degree of crystal growth.…”

Section: Properties Of P(lactate-co-3hb)mentioning

confidence: 95%