Lack of Response of Murine Peritoneal Macrophages to <i>in vitro</i> Activation by Muramyl Dipeptide (MDP)

Nagao, Shigeki; Akagawa, Kiyoko S.; Yamada, Kazuo; Yagawa, Katsuro; Tokunaga, Tohru; Kotani, Shozo

doi:10.1111/j.1348-0421.1990.tb01013.x

Cited by 24 publications

(17 citation statements)

References 28 publications

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“…25 However, Nagao et al 26 claimed that peptidoglycan and MDP failed to induced IL-1 and TNF in murine macrophage cultures, which is contrary to the positive results with guinea-pig macrophages and human monocyte cultures. Controversy still exists as to the responsiveness of murine macrophages to peptidoglycan and MDP.…”

Section: Discussioncontrasting

confidence: 49%

Induction of inflammatory cytokines by a soluble moiety prepared from an enzyme lysate of Actinomyces viscosus cell walls

Takada¹,

Kimura²,

Hamada³

1993

Journal of Medical Microbiology

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Summary.A carbohydrate-rich and immunobiologically active component, M 1 Av, was prepared from an M-1 endo-N-acetylmuramidase digest of cell walls of Actinomyces viscosus ATCC 19246 by CM Sephadex C-25 and Sephadex G-100 chromatography. M 1Av stimulated thioglycolate-induced peritoneal macrophages from C3H/HeN and C3H/HeJ mice to release cell-free tumour necrosis factor (TNF) and interleukin (1L)-6, and a cell-associated thymocyte activating factor, probably IL-1. An intravenous (i.v.) injection of M 1Av induced increased levels of TNF and IL-6 in the serum of C3H/HeN mice that had been primed with 1OOpg of muramyldipeptide (MDP) i.v. However, MlAv did not induce TNF release in C3H/HeJ mice similarly primed with MDP. Simultaneous administration of M1 Av (100 pg, i.v.) and galactosamine (18 mg, intraperitoneally) killed C3H/HeN, but not C3H/HeJ mice. MlAv was shown to be practically free of endotoxin by the Limulus test. These findings indicate that the solubilised A. viscosus cell-wall carbohydrate moiety induced inflammatory cytokines both in vitro and in vivo.

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Section: Discussioncontrasting

confidence: 49%

Induction of inflammatory cytokines by a soluble moiety prepared from an enzyme lysate of Actinomyces viscosus cell walls

Takada¹,

Kimura²,

Hamada³

1993

Journal of Medical Microbiology

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“…These two studies both consider that the used compounds are recognized equally well by mouse and human NOD1. However, it must be noted that species dependency of MDP sensing has been reported [10,23], opening up the possibility that species differences may exist for agonist specificity of both NOD1 and NOD2.…”

Section: Discussionmentioning

confidence: 99%

Synergistic stimulation of human monocytes and dendritic cells by Toll-like receptor 4 and NOD1- and NOD2-activating agonists

et al. 2005

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Muropeptides are degradation products of bacterial peptidoglycan (PG) sensed by nucleotide-binding oligomerization domain 1 (NOD1) and NOD2, members of a recently discovered family of pattern recognition molecules (PRM). One of these muropeptides, muramyl dipeptide (MDP) mediates cell signaling by NOD2, exerts adjuvant activity and synergizes with lipopolysaccharide (LPS) to induce proinflammatory responses in vitro and in vivo. In contrast, few and contradictory results exist about the stimulatory capacity of NOD1 agonists. Thus, the ability of NOD1 (MurNAc-L-Ala-c-D-Glu-meso-diaminopimelic acid, MtriDAP) and NOD2 (MurNAc-LAla-D-isoGln, MDP; MurNAc-L-Ala-c-D-Glu-L-Lys, MtriLYS) agonists to activate primary human myeloid cells was examined. We show that both CD14 + monocytes and CD1a + immature dendritic cells (DC) express NOD1 and NOD2 mRNA. Stimulation of primary human monocytes and DC with highly purified muropeptides (MtriDAP, MDP and MtriLYS) induces release of pro-inflammatory cytokines. We reveal here that NOD1 as well as NOD2 agonists act cooperatively with LPS to stimulate the release of both proand anti-inflammatory cytokines in these myeloid cell subsets. Finally, we report that NOD1 as well as NOD2 agonists synergize with sub-active doses of LPS to induce DC maturation, demonstrating that NOD agonists act cooperatively with molecules sensed by Toll-like receptor 4 to instruct the onset of adaptive immune responses.

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“…Nagao et al (23,24) reported species dependency of in vitro macrophage activation by peptidoglycans and MDP; guinea pig and rat cells were responsive, but murine cells were not. In human peripheral blood mononuclear cell cultures, MDP is a powerful cytokine inducer comparable to LPS.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effect of Muramyldipeptide with Lipopolysaccharide or Lipoteichoic Acid To Induce Inflammatory Cytokines in Human Monocytic Cells in Culture

et al. 2001

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Lack of Response of Murine Peritoneal Macrophages to in vitro Activation by Muramyl Dipeptide (MDP)

Abstract: Peritoneal exudate macrophages from mice, rats, and guinea pigs were assessed using six different parameters of macrophage activation to determine whether

Cited by 24 publications

References 28 publications

Induction of inflammatory cytokines by a soluble moiety prepared from an enzyme lysate of Actinomyces viscosus cell walls

Induction of inflammatory cytokines by a soluble moiety prepared from an enzyme lysate of Actinomyces viscosus cell walls

Synergistic stimulation of human monocytes and dendritic cells by Toll-like receptor 4 and NOD1- and NOD2-activating agonists

Synergistic Effect of Muramyldipeptide with Lipopolysaccharide or Lipoteichoic Acid To Induce Inflammatory Cytokines in Human Monocytic Cells in Culture

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