Lack of evidence supporting a role of IFN-β and TGF-β in differential polarization of Bordetella pertussis specific-T cell responses

Antunes, Ricardo da Silva; Quiambao, Lorenzo; Soldevila, Ferran; Sutherland, Aaron; Peters, Bjoern; Sette, Alessandro

doi:10.1016/j.cyto.2020.155313

Cited by 11 publications

(12 citation statements)

References 39 publications

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“…The specific MPs were prepared by sequential lyophilization of overlapping 15-mers by 10 pooled by protein combinations, as previously reported [ 16 ]. We also utilized two previously described MPs as additional controls encompassing T cell epitopes from Bordetella pertussis (PT) [ 19 , 20 ] and cytomegalovirus (CMV) [ 15 ].…”

Section: Resultsmentioning

confidence: 99%

“…We also analyzed CD4 T cell reactivity to two previously described MPs encompassing T cell epitopes from Bordetella pertussis (PT) [ 19 , 20 ] and cytomegalovirus (CMV) [ 15 ] as controls. The results are shown in Figure S3 (panels A and C) .…”

Section: Resultsmentioning

confidence: 99%

“…Detailed information of the MPs composition and peptide sequences were listed in Tables S1–S4 . We also utilized, as additional controls, two previously described MPs encompassing T cell epitopes from Bordetella pertussis (PT) [ 19 , 20 ] and Cytomegalovirus (CMV) [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

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Balanced Cellular and Humoral Immune Responses Targeting Multiple Antigens in Adults Receiving a Quadrivalent Inactivated Influenza Vaccine

Grifoni

Sutherland

et al. 2021

Vaccines

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The role of T cell immunity has been acknowledged in recent vaccine development and evaluation. We tested the humoral and cellular immune responses to Flucelvax®, a quadrivalent inactivated seasonal influenza vaccine containing two influenza A (H1N1 Singapore/GP1908/2015 IVR-180 and H3N2 North Carolina/04/2016) and two influenza B (Iowa/06/2017 and Singapore/INFTT-16-0610/2016) virus strains, using peripheral blood mononuclear cells stimulated by pools of peptides overlapping all the individual influenza viral protein components. Baseline reactivity was detected against all four strains both at the level of CD4 and CD8 responses and targeting different proteins. CD4 T cell reactivity was mostly directed to HA/NA proteins in influenza B strains, and NP/M1/M2/NS1/NEP proteins in the case of the Influenza A strains. CD8 responses to both influenza A and B viruses preferentially targeted the more conserved core viral proteins. Following vaccination, both CD4 and CD8 responses against the various influenza antigens were increased in day 15 to day 91 post vaccination period, and maintained a Th1 polarized profile. Importantly, no vaccine interference was detected, with the increased responses balanced across all four included viral strains for both CD4 and CD8 T cells, and targeting HA and multiple additional viral antigens.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Balanced Cellular and Humoral Immune Responses Targeting Multiple Antigens in Adults Receiving a Quadrivalent Inactivated Influenza Vaccine

Grifoni

Sutherland

et al. 2021

Vaccines

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“…This is in contrast to influenza, where the vaccine composition and peptide epitopes change every year, or to other ubiquitous pathogens (such as CMV) where responsiveness is dependent on the individuals being chronically infected. Furthermore, the PT peptide pool is associated with HLA class II restricted CD4 responses, like α-syn, and it has been extensively characterized 11,[20][21][22][23] . α-Syn, in contrast, is a small self-antigen with only 11 defined T cell epitopes, and so we expected to find a much narrower repertoire for α-syn.…”

Section: Discussionmentioning

confidence: 99%

The TCR repertoire of α-synuclein-specific T cells in Parkinson’s disease is surprisingly diverse

Singhania

Pham

Dhanwani

et al. 2021

Sci Rep

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The self-antigen α-synuclein (α-syn) was recently shown to be associated with Parkinson’s disease (PD). Here we mapped the T cell receptor (TCR) repertoire of α-syn-specific T cells from six PD patients. The self-antigen α-syn-specific repertoire was compared to the repertoire of T cells specific for pertussis (PT), as a representative foreign antigen that most individuals are exposed to, revealing that the repertoire for α-syn was as diverse as the repertoire for PT. The diversity of PT-specific clonotypes was similar between individuals with PD diagnosis and age-matched healthy controls. We found that the TCR repertoire was specific to each PD patient, and no shared TCRs among patients were defined, likely due to differences in HLA expression that select for different subsets of epitope-specific TCR rearrangements. This study provides the first characterization of α-syn-specific TCR clonotypes in individuals with PD. Antigen-specific TCRs can serve as immunotherapeutics and diagnostics, and means to track longitudinal changes in specific T cells, and disease progression.

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“…Observing differences in antibody isotype polarization decades after the initial priming is interesting and was also observed at the level of memory T cell responses ( 22 ). Although of high interest, the examination of memory T cell responses at the antigen-specific level is beyond the scope of this study and has already been addressed in our previous studies ( 22 , 54 ).…”

Section: Discussionmentioning

confidence: 99%

A system-view of Bordetella pertussis booster vaccine responses in adults primed with whole-cell versus acellular vaccine in infancy

et al. 2021

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Lack of evidence supporting a role of IFN-β and TGF-β in differential polarization of Bordetella pertussis specific-T cell responses

Cited by 11 publications

References 39 publications

Balanced Cellular and Humoral Immune Responses Targeting Multiple Antigens in Adults Receiving a Quadrivalent Inactivated Influenza Vaccine

Balanced Cellular and Humoral Immune Responses Targeting Multiple Antigens in Adults Receiving a Quadrivalent Inactivated Influenza Vaccine

The TCR repertoire of α-synuclein-specific T cells in Parkinson’s disease is surprisingly diverse

A system-view of Bordetella pertussis booster vaccine responses in adults primed with whole-cell versus acellular vaccine in infancy

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