Lack of Association Between Titers of HAl Antibody and Whole-Virus ELISA Values for Patients with Congenital Rubella Syndrome

Hancock, Elizabeth J.; Pot, Kees H.; Puterman, Martin L.; Tingle, Aubrey J.

doi:10.1093/infdis/154.6.1031

Cited by 10 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HI or microneutralization assays may severely underestimate the degree of immunity by omitting the detection of antibodies raised against other viral proteins [15]. In our studies, NI titer did not positively correlate with HI titers ( P ≤ 0.84) nor was associated with IgG titers to whole virus (data not shown) illustrating the complexity of the antibody response to influenza.…”

Section: Discussioncontrasting

confidence: 48%

Inactivated Seasonal Influenza Vaccines Increase Serum Antibodies to the Neuraminidase of Pandemic Influenza A(H1N1) 2009 Virus in an Age‐Dependent Manner

Marcelin¹,

Bland²,

Negovetich³

et al. 2010

J INFECT DIS

View full text Add to dashboard Cite

Pre-existing antibodies to the hemagglutinin of pandemic A (H1N1) 2009 influenza virus (pH1N1) positively correlates with age. The impact of contemporary seasonal influenza vaccines on establishing immunity to other pH1N1 proteins is unknown. We measured serum antibodies to the neuraminidase of pandemic H1N1 (pNA) in adults prior to and after vaccination with seasonal trivalent inactivated influenza vaccines. Serum antibodies to pNA were observed in all age groups; however, vaccination elevated pNA antibodies in predominately the elderly. Therefore, contemporary seasonal vaccines likely contribute to reduction of pH1N1 associated disease in older individuals.

show abstract

Section: Discussioncontrasting

confidence: 48%

Inactivated Seasonal Influenza Vaccines Increase Serum Antibodies to the Neuraminidase of Pandemic Influenza A(H1N1) 2009 Virus in an Age‐Dependent Manner

Marcelin¹,

Bland²,

Negovetich³

et al. 2010

J INFECT DIS

View full text Add to dashboard Cite

show abstract

“…Several limitations exist when protective responses are determined by antibody responses to HA alone. Measuring exclusively HA antibodies may severely underestimate the degree of immunity by omitting the detection of antibodies raised against other viral proteins [35]. In our previous study [20], we discovered that antibodies to NA did not positively correlate with antibodies to HA, thus supporting this idea.…”

Section: Discussionmentioning

confidence: 73%

A Contributing Role for Anti-Neuraminidase Antibodies on Immunity to Pandemic H1N1 2009 Influenza A Virus

et al. 2011

View full text Add to dashboard Cite

BackgroundExposure to contemporary seasonal influenza A viruses affords partial immunity to pandemic H1N1 2009 influenza A virus (pH1N1) infection. The impact of antibodies to the neuraminidase (NA) of seasonal influenza A viruses to cross-immunity against pH1N1 infection is unknown.Methods and ResultsAntibodies to the NA of different seasonal H1N1 influenza strains were tested for cross-reactivity against A/California/04/09 (pH1N1). A panel of reverse genetic (rg) recombinant viruses was generated containing 7 genes of the H1N1 influenza strain A/Puerto Rico/08/34 (PR8) and the NA gene of either the pandemic H1N1 2009 strain (pH1N1) or one of the following contemporary seasonal H1N1 strains: A/Solomon/03/06 (rg Solomon) or A/Brisbane/59/07 (rg Brisbane). Convalescent sera collected from mice infected with recombinant viruses were measured for cross-reactive antibodies to pH1N1 via Hemagglutinin Inhibition (HI) or Enzyme-Linked Immunosorbent Assay (ELISA). The ectodomain of a recombinant NA protein from the pH1N1 strain (pNA-ecto) was expressed, purified and used in ELISA to measure cross-reactive antibodies. Analysis of sera from elderly humans immunized with trivalent split-inactivated influenza (TIV) seasonal vaccines prior to 2009 revealed considerable cross-reactivity to pNA-ecto. High titers of cross-reactive antibodies were detected in mice inoculated with either rg Solomon or rg Brisbane. Convalescent sera from mice inoculated with recombinant viruses were used to immunize naïve recipient Balb/c mice by passive transfer prior to challenge with pH1N1. Mice receiving rg California sera were better protected than animals receiving rg Solomon or rg Brisbane sera.ConclusionsThe NA of contemporary seasonal H1N1 influenza strains induces a cross-reactive antibody response to pH1N1 that correlates with reduced lethality from pH1N1 challenge, albeit less efficiently than anti-pH1N1 NA antibodies. These findings demonstrate that seasonal NA antibodies contribute to but are not sufficient for cross-reactive immunity to pH1N1.

show abstract

“…Alternatively, this domain, as an adjacent epitope to the previously established neutralization and HAI domains, may also be recognized antigenically in the context of an effective immune response to RV. It is well established that congenital RV infection may be associated with long-term virus persistence in tissues and excretion of RV postnatally, as well as abnormal patterns of RV immunoreactivity (3,6,12,16). RV persistence in CRS patients may be due to development of selective immunologic tolerance to neutralization domains on RV El protein as a result of exposure of the immature fetal immune system to RV.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, lack of responsiveness to biologically relevant regions of RV El protein may lead to failed immunity to RV and reinfection. Numerous cases concerning the lack of association of immunity as determined by gold standard techniques such as HAI and neutralizing antibody tests and RV reinfection have been documented (9,12,15,21). This is of considerable concern because of the risk of RV reinfection in pregnant women (9,13).…”

Section: Discussionmentioning

confidence: 99%

Characterization of rubella virus-specific antibody responses by using a new synthetic peptide-based enzyme-linked immunosorbent assay

Mitchell

Zhang

et al. 1992

J Clin Microbiol

View full text Add to dashboard Cite

Rubella virus (RV)-specific immunoglobulin G antibodies were studied by enzyme-linked immunosorbent assay (ELISA) techniques in sera from RV (RA 27/3)-vaccinated individuals, patients experiencing natural RV infection, congenital rubella syndrome patients, and individuals failing to respond to repeated RV immunization. Results obtained by using whole-RV ELISAs (detergent-solubilized M33 strain or intact Gilchrist strain) and hemagglutination inhibition (HAI) and neutralization (NT) assays were compared with results obtained with the same sera by using ELISAs employing a synthetic peptide, BCH-178, representing a putative neutralization domain on the RV El protein. Murine RV El-specific monoclonal antibodies with HAI and NT activities exhibited strong reactivity in ELISAs with BCH-178 peptide. In sera from RA 27/3-vaccinated individuals collected at 0 (prevaccine), 1, 2, 3, 4, 5, 6, 12, and 24 to 52 weeks postvaccine, the development of El-peptide-reactive antibodies closely paralleled increases in RV-specific antibodies measured by whole-RV ELISAs and HAI and NT assays. Similarly, sequential serum samples obtained from patients during acute and convalescent phases of natural RV infection showed a coordinate increase in RV-specific antibodies as measured by whole-RV and peptide ELISAs. Conversely, congenital rubella syndrome patient sera, although exhibiting high levels of antibody in whole-RV ELISAs, had little or no antibody directed to the neutralization domain peptide. Sera from patients failing to respond to repeated RV immunization contained very low levels of RV-specific antibody in all ELISAs. Our results suggest that the sequence represented by BCH-178 peptide may be a previously unidentified neutralization epitope for human antibodies on the RV El protein and may prove useful in determining effective RV immunity.

show abstract

Lack of Association Between Titers of HAl Antibody and Whole-Virus ELISA Values for Patients with Congenital Rubella Syndrome

Cited by 10 publications

References 0 publications

Inactivated Seasonal Influenza Vaccines Increase Serum Antibodies to the Neuraminidase of Pandemic Influenza A(H1N1) 2009 Virus in an Age‐Dependent Manner

Inactivated Seasonal Influenza Vaccines Increase Serum Antibodies to the Neuraminidase of Pandemic Influenza A(H1N1) 2009 Virus in an Age‐Dependent Manner

A Contributing Role for Anti-Neuraminidase Antibodies on Immunity to Pandemic H1N1 2009 Influenza A Virus

Characterization of rubella virus-specific antibody responses by using a new synthetic peptide-based enzyme-linked immunosorbent assay

Contact Info

Product

Resources

About