Laccase stabilized on β-D-glucan films on the surface of carbon black/gold nanoparticles: A new platform for electrochemical biosensing

Mattos, Gabriel Junquetti; Moraes, Jaqueline Tobias; Barbosa, Eduardo C. M.; Camargo, Pedro Henrique Cury de; Dekker, Robert F. H.; Barbosa-Dekker, Aneli M.; Sartori, Elen Romão

doi:10.1016/j.bioelechem.2019.05.002

Cited by 28 publications

(7 citation statements)

References 28 publications

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“…Biosensors are analytical devices developed for the detection of compounds, and analysis of parameters of biological interest. The unique redox properties of laccases, especially those produced from fungi, make them interesting tools to be applied for bioelectrochemical purposes in biosensor devices . The potential of prokaryotic laccases in electrochemistry has been not fully understood yet.…”

Section: Laccases As Versatile Enzymes: Traditional and New Industriamentioning

confidence: 99%

Laccases as versatile enzymes: from industrial uses to novel applications

Moreno

Ibarra²,

Eugenio³

et al. 2019

J of Chemical Tech & Biotech

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The application of enzymes offers an enormous potential in the improvement of existing industrial procedures and in the establishment of new processes for obtaining high-added value products. Enzymes provide cleaner and more efficient industrial processes and contribute to the sustainability concept. In this sense, laccases are very versatile biocatalysts currently used in food, textile and pulp and paper sectors among others. During the last years, scientific efforts have been diverted to the exploitation of such interesting enzymes in novel fields like lignocellulosic biorefineries, biosensors or enzymatic biofuel cells. This review provides a general vision of the use of laccase enzymes describing their main characteristics and mode of action. Furthermore, their current uses in industrial processes are summarized and the most novel potential application of laccases are revealed. The increasing interest on laccases is also demonstrated by the research efforts on enzyme engineering as it is detailed in this review. wileyonlinelibrary.com/jctb www.soci.org AD Moreno et al. J Chem Technol Biotechnol 2020; 95: 481-494

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Section: Laccases As Versatile Enzymes: Traditional and New Industriamentioning

confidence: 99%

Laccases as versatile enzymes: from industrial uses to novel applications

Moreno

Ibarra²,

Eugenio³

et al. 2019

J of Chemical Tech & Biotech

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“…Often, the CB electroanalytical features, both for direct sensing and bio-receptor immobilization, are improved by using gold nanoparticles (AuNPs). The CB-AuNPs’ synergy has been exploited to improve electron transfer and the electroanalytical detection of different analytes as phenols [ 19 , 35 , 36 , 37 , 38 ], sugars [ 36 ], and pollutant metals [ 39 , 40 , 41 ]. To form CB-AuNP nanocomposites, different strategies have been proposed.…”

Section: Introductionmentioning

confidence: 99%

“…To form CB-AuNP nanocomposites, different strategies have been proposed. The most-employed strategies include the drop-casting of preformed AuNPs [ 35 , 36 , 37 , 38 , 39 , 40 ] and the AuNPs’ electrosynthesis onto electrodes previously modified with CB [ 41 , 42 ]. These approaches present some disadvantages, mainly due to the random and/or non-reproducible CB modifications, the tendency to form nanoparticle aggregates, the risk of the formation of metallic micro islands, and the persistence of the metallic precursor.…”

Section: Introductionmentioning

confidence: 99%

Carbon Black Functionalized with Naturally Occurring Compounds in Water Phase for Electrochemical Sensing of Antioxidant Compounds

et al. 2022

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A new sustainable route to nanodispersed and functionalized carbon black in water phase (W-CB) is proposed. The sonochemical strategy exploits ultrasounds to disaggregate the CB, while two selected functional naturally derived compounds, sodium cholate (SC) and rosmarinic acid (RA), act as stabilizing agents ensuring dispersibility in water adhering onto the CB nanoparticles’ surface. Strategically, the CB-RA compound is used to drive the AuNPs self-assembling at room temperature, resulting in a CB surface that is nanodecorated; further, this is achieved without the need for additional reagents. Electrochemical sensors based on the proposed nanomaterials are realized and characterized both morphologically and electrochemically. The W-CBs’ electroanalytical potential is proved in the anodic and cathodic window using caffeic acid (CF) and hydroquinone (HQ), two antioxidant compounds that are significant for food and the environment. For both antioxidants, repeatable (RSD ≤ 3.3%; n = 10) and reproducible (RSD ≤ 3.8%; n = 3) electroanalysis results were obtained, achieving nanomolar detection limits (CF: 29 nM; HQ: 44 nM). CF and HQ are successfully determined in food and environmental samples (recoveries 97–113%), and also in the presence of other phenolic classes and HQ structural isomers. The water dispersibility of the proposed materials can be an opportunity for (bio) sensor fabrication and sustainable device realization.

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“…In the last few years, a number of electrochemical biosensors combining phenol oxidases with a variety of materials, such as polymeric matrices [5,6] or nanomaterials, Sensors 2021, 21, 899 2 of 13 have been developed [7,8]. Carbon nanotubes (CNTs) [9,10] graphene [11,12], carbon fibers [13], metallic nanoparticles, metal oxide nanoparticles, nanostars [14][15][16][17][18], molecular imprinted polymers [19], and nanostructured thin films [20,21] have all been shown to be excellent electron mediators. One-dimensional (1D) nanostructures can be an interesting alternative to zero-dimensional (OD) nanoparticles due to their high surface-to-volume ratios.…”

Section: Introductionmentioning

confidence: 99%

Silver Nanowires as Electron Transfer Mediators in Electrochemical Catechol Biosensors

Salvo-Comino

Martín-Pedrosa

Garcı́a

et al. 2021

Sensors

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The integration of nanomaterials as electron mediators in electrochemical biosensors is taking on an essential role. Due to their high surface-to-volume ratio and high conductivity, metallic nanowires are an interesting option. In this paper, silver nanowires (AgNWs) were exploited to design a novel catechol electrochemical biosensor, and the benefits of increasing the aspect ratio of the electron mediator (nanowires vs. nanoparticles) were analyzed. Atomic force microscopy (AFM) studies have shown a homogeneous distribution of the enzyme along the silver nanowires, maximizing the contact surface. The large contact area promotes electron transfer between the enzyme and the electrode surface, resulting in a Limit of Detection (LOD) of 2.7 × 10−6 M for tyrosinase immobilized onto AgNWs (AgNWs-Tyr), which is one order of magnitude lower than the LOD of 3.2 × 10−5 M) obtained using tyrosinase immobilized onto silver nanoparticles (AgNPs-Tyr). The calculated KM constant was 122 mM. The simultaneous use of electrochemistry and AFM has demonstrated a limited electrochemical fouling that facilitates stable and reproducible detection. Finally, the biosensor showed excellent anti-interference characteristics toward the main phenols present in wines including vanillin, pyrogallol, quercetin and catechin. The biosensor was able to successfully detect the presence of catechol in real wine samples. These results make AgNWs promising elements in nanowired biosensors for the sensitive, stable and rapid voltammetric detection of phenols in real applications.

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Laccase stabilized on β-D-glucan films on the surface of carbon black/gold nanoparticles: A new platform for electrochemical biosensing

Cited by 28 publications

References 28 publications

Laccases as versatile enzymes: from industrial uses to novel applications

Laccases as versatile enzymes: from industrial uses to novel applications

Carbon Black Functionalized with Naturally Occurring Compounds in Water Phase for Electrochemical Sensing of Antioxidant Compounds

Silver Nanowires as Electron Transfer Mediators in Electrochemical Catechol Biosensors

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