Laccase-loaded magnetic dialdehyde inulin nanoparticles as an efficient heterogeneous natural polymer-based biocatalyst for removal and detoxification of ofloxacin

Shokri, Mahtab; Mojtabavi, Somayeh; Jafari-Nodoushan, Hossein; Vojdanitalab, Khashayar; Golshani, Shiva; Jahandar, Hoda; Faramarzi, Mohammad Ali

doi:10.1007/s10532-022-09994-x

Cited by 7 publications

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results showed that the immobilization did not increase the stability of the enzyme but instead led to a loss in the stability at high temperatures, together with enzyme leaching and degradation. In a different approach, in which a laccase was covalently immobilized into the surface of inulin-coated magnetic nanoparticles, approximately a 1.5-fold increase in thermal stability was conferred to the enzyme, as shown by residual activity after a 3 h-incubation at 75°C and pH ranging from 2.5 to 7.5 [ 58 ]. Inulin is also a fructan-based polymer, as levan.…”

Section: Resultsmentioning

confidence: 99%

Thermostabilization of a fungal laccase by entrapment in enzymatically synthesized levan nanoparticles

Alishah Aratboni,

Martinez,

Olvera

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

In this work, we present a comprehensive investigation of the entrapment of laccase, a biotechnologically relevant enzyme, into levan-based nanoparticles (LNPs). The entrapment of laccase was achieved concomitantly with the synthesis of LNP, catalyzed by a truncated version of a levansucrase from Leuconostoc mesenteroides. The study aimed to obtain a biocompatible nanomaterial, able to entrap functional laccase, and characterize its physicochemical, kinetic and thermal stability properties. The experimental findings demonstrated that a colloidal stable solution of spherically shaped LNP, with an average diameter of 68 nm, was obtained. An uniform particle size distribution was observed, according to the polydispersity index determined by DLS. When the LNPs synthesis was performed in the presence of laccase, biocatalytically active nanoparticles with a 1.25-fold larger diameter (85 nm) were obtained, and a maximum load of 243 μg laccase per g of nanoparticle was achieved. The catalytic efficiency was 972 and 103 (μM·min)-1, respectively, for free and entrapped laccase. A decrease in kcat values (from 7050 min-1 to 1823 min-1) and an increase in apparent Km (from 7.25 μM to 17.73 μM) was observed for entrapped laccase, compared to the free enzyme. The entrapped laccase exhibited improved thermal stability, retaining 40% activity after 1 h-incubation at 70°C, compared to complete inactivation of free laccase under the same conditions, thereby highlighting the potential of LNPs in preserving enzyme activity under elevated temperatures. The outcomes of this investigation significantly contribute to the field of nanobiotechnology by expanding the applications of laccase and presenting an innovative strategy for enhancing enzyme stability through the utilization of fructan-based nanoparticle entrapments.

show abstract

Section: Resultsmentioning

confidence: 99%

Thermostabilization of a fungal laccase by entrapment in enzymatically synthesized levan nanoparticles

Alishah Aratboni,

Martinez,

Olvera

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…It is worth to mention that both, biochar from various sources and other biomass-derived products such as coconut fiber, quinoa husks, rice husks, switchgrass, wood from Eucalyptus sp., spent grain, and others, as well as synthetic biopolymers have been used as Lac carriers for the removal of nonchlorinated compounds such as antibiotics (Shokri et al, 2022;Zdarta et al, 2020;Zou et al, 2023), dyes (Ariaeenejad et al, 2022;Aricov et al, 2020;Gao et al, 2022;Ghodake et al, 2018;Girelli et al, 2021;Pandey et al, 2022Pandey et al, , 2023dos Santos et al, 2022;Tuncay & Yagar, 2020), nitrogen-containing organic compounds such as carbazole and indole (Gu et al, 2020), precursors for industrial use such as bisphenol A (Bilal, Jing, et al, 2019;Lassouane et al, 2019;Zhang et al, 2022), anthracene (Imam et al, 2021), petroleum contaminants (Zhou et al, 2023), phenolic compounds in fruit juices (Bezerra et al, 2015), naturally occurring syringic acid (Scuto et al, 2023), and estrogen (Rybarczyk T A B L E 4 Compilation of studies dealing with the removal of OC compounds using immobilized Lac on biochar as material supports. | 443 et al, 2023;Vázquez et al, 2023).…”

Section: Fungal Lac Immobilizationmentioning

confidence: 99%

Immobilizing white‐rot fungi laccase: Toward bio‐derived supports as a circular economy approach in organochlorine removal

Serbent,

Magario,

Saux

2023

Biotech & Bioengineering

View full text Add to dashboard Cite

Despite their high persistence in the environment, organochlorines (OC) are widely used in the pharmaceutical industry, in plastics, and in the manufacture of pesticides, among other applications. These compounds and the byproducts of their decomposition deserve attention and efficient proposals for their treatment. Among sustainable alternatives, the use of ligninolytic enzymes (LEs) from fungi stands out, as these molecules can catalyze the transformation of a wide range of pollutants. Among LEs, laccases (Lac) are known for their efficiency as biocatalysts in the conversion of organic pollutants. Their application in biotechnological processes is possible, but the enzymes are often unstable and difficult to recover after use, driving up costs. Immobilization of enzymes on a matrix (support or solid carrier) allows recovery and stabilization of this catalytic capacity. Agricultural residual biomass is a passive environmental asset. Although underestimated and still treated as an undesirable component, residual biomass can be used as a low‐cost adsorbent and as a support for the immobilization of enzymes. In this review, the adsorption capacity and immobilization of fungal Lac on supports made from residual biomass, including compounds such as biochar, for the removal of OC compounds are analyzed and compared with the use of synthetic supports. A qualitative and quantitative comparison of the reported results was made. In this context, the use of peanut shells is highlighted in view of the increasing peanut production worldwide. The linkage of methods with circular economy approaches that can be applied in practice is discussed.

show abstract

Towards oxidoreductase-based processes for the removal of antibiotics from wastewater

de Boer,

Schäffer,

Moreira

2023

Rev Environ Sci Biotechnol

View full text Add to dashboard Cite

The occurrence of antibiotics in surface waters is an alarming issue that can be addressed by advanced wastewater treatment technologies. Among them, enzymatic treatment is an emerging technology claimed to provide prospective benefits in terms of efficiency, controllability, and safety. This review illustrates the current state of research focused on enzyme-based approaches for pollutant abatement, specifically on the most critical classes of antibiotics (e.g. tetracyclines, sulfonamides, fluoroquinolones). In addition to providing an overview of the efficiency both in terms of compound removal as well as toxicity reduction, we critically analyze if selected reaction conditions, such as the pH, temperature and water matrix are representative for real-case scenarios. Enzyme immobilization strategies onto inorganic, organic and composite materials are analyzed in terms of their effect on enzyme stability and activity. Their feasibility to be applied in future processes was also evaluated. We found that adequate kinetic description of target compound removal by sufficiently detailed models is still scarce even though it will be key for successful conceptualization of treatment processes. Considering that only a few studies have been conducted at scales above 100 mL, we present the investigated reactor configurations which are at the forefront of further scale-up. The systematic approach presented in this manuscript, which aims to critically evaluate the feasibility to implement enzymatic processes for the removal of antibiotics, can be adapted for other types of recalcitrant compounds targeted by oxidoreductases. Intensified research in the recommended areas will contribute to the development of enzyme-based processes which can complement other advanced wastewater treatment processes. Graphical abstract

show abstract

Laccase-loaded magnetic dialdehyde inulin nanoparticles as an efficient heterogeneous natural polymer-based biocatalyst for removal and detoxification of ofloxacin

Cited by 7 publications

References 36 publications

Thermostabilization of a fungal laccase by entrapment in enzymatically synthesized levan nanoparticles

Thermostabilization of a fungal laccase by entrapment in enzymatically synthesized levan nanoparticles

Immobilizing white‐rot fungi laccase: Toward bio‐derived supports as a circular economy approach in organochlorine removal

Towards oxidoreductase-based processes for the removal of antibiotics from wastewater

Contact Info

Product

Resources

About