Laboratory diagnosis of tuberculosis: literature review

Costa, Ronaldo Rodrigues da; Silva, Marcio Roberto; Gonçalves, Isabel Cristina Rocha Hipólito

doi:10.5935/2238-3182.20180137

Cited by 2 publications

(2 citation statements)

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“…The same error occurs while performing the culture test and the diagnostic test, which is considered the TB standard [32,33], in which 87.2% were not performed. Not performing the culture analysis is a "bad culture", which can lead to a blind treatment especially for MDR-TB cases or for pulmonary diseases caused by non-tuberculous mycobacterias (NTM) (mycobacteriosis), such as Mycobacterium kansasii, which are undistinguished from TB clinically or through microscopy.…”

Section: Discussionmentioning

confidence: 98%

A Clinical-Epidemiological and Geospatial Study of Tuberculosis in a Neglected Area in the Amazonian Region Highlights the Urgent Need for Control Measures

Mesquita

Conceição

Monteiro

et al. 2021

IJERPH

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Tuberculosis (TB) is an infectious communicable disease, which despite global efforts, still needs special attention in regions with difficult access. This study aims to describe the spatial and epidemiological trends of TB incidences from 2013 to 2018 in Marajó Island, the Amazonian region, Pará, Brazil. We have obtained secondary data from the Brazilian TB databases and performed geospatial and statistical analyses on the data for new TB cases, relapses, and re-admissions. From 2013 to 2018, 749 new cases were reported, in which the diagnostics (culture) was not performed for 652 (87.2%) patient samples, the drug resistance test (DST) was performed for nine (1.2%) samples, and one (0.13%) was multidrug-resistant TB (MDR-TB). The rapid molecular testing (RMT) was performed on 40 (5.3%) patient samples, with results indicating that eight (20%) were susceptible to rifampicin and two (5%) were rifampicin resistant. Overall, the cure rate was 449 (66.7%), while relapses and re-admissions were 41 and 44, respectively. On the geospatial distribution, the municipality of Soure stands out with a high number of incidences, relapses, and re-admissions. Spatially, the eight MDR cases were randomly distributed. Our data highlight the urgent need for TB control measures in this region, by introducing the Xpert-Ultra® MTB/RIF (Cepheid, Sunnyvale, CA, USA) and Ogawa-Kudoh.

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Section: Discussionmentioning

confidence: 98%

A Clinical-Epidemiological and Geospatial Study of Tuberculosis in a Neglected Area in the Amazonian Region Highlights the Urgent Need for Control Measures

Mesquita

Conceição

Monteiro

et al. 2021

IJERPH

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“…Agreement with AFB testing was substantial (Kappa = 0.62), that is, 11 AFB negative samples were detected by PCR. Greater detection by qPCR was already expected, due to the need for a high bacterial load (around 10,000 bacilli per mL of sample) for sputum smear positivity, (28) unlike qPCR, where low DNA concentrations of NTMs could already be amplified. (16,29) When testing the 14 samples from TB positive patients, no amplification was detected, which may allow the use of qPCR to differentiate between TB and NTM.…”

Section: Discussionmentioning

confidence: 99%

Detection of non-tuberculosus mycobacteria (NTMs) in lung samples using 16S rRNA

Morais

Bello

Costi

et al. 2022

Mem. Inst. Oswaldo Cruz

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BACKGROUND Non-tuberculous mycobacteria (NTMs) cause diseases known as mycobacteriosis and are an important cause of morbidity and mortality. The diagnosis of pulmonary disease caused by NTM is hampered by its clinical similarity with tuberculosis (TB) and by the lack of an accurate and rapid laboratory diagnosis. OBJECTIVES Detect DNA from NTMs directly from lung samples using real-time polymerase chain reaction (qPCR) for amplification of 16S rRNA. Additionally, DNA sequencing ( hsp65 and rpoB genes) was used to identify the species of MNTs. METHODS A total of 68 sputum samples (54 with suspected NTMs and 14 with TB) from patients treated at a referral hospital were used. FINDINGS Of these, 27/54 (50%) were qPCR positive for NTMs and 14/14 TB patients (controls) were qPCR negative with an almost perfect concordance ( Kappa of 0.93) with the Mycobacterium spp. culture. Sequencing confirmed the presence of NTM in all positive samples. The most common species was Mycobacterium gordonae (33%), followed by Mycobacterium abscessus (26%), Mycobacterium fortuitum (22%), Mycobacterium avium (15%) and Mycobacterium peregrinum (4%). MAIN CONCLUSIONS The qPCR technique for detecting NTMs targeting 16S rRNA has the potential to detect NTMs and rapidly differentiate from Mycobacterium tuberculosis . However, it is necessary to identify the species to help in the differential diagnosis between disease and contamination, and to guide the choice of the therapeutic scheme.

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Laboratory diagnosis of tuberculosis: literature review

Cited by 2 publications

References 0 publications

A Clinical-Epidemiological and Geospatial Study of Tuberculosis in a Neglected Area in the Amazonian Region Highlights the Urgent Need for Control Measures

A Clinical-Epidemiological and Geospatial Study of Tuberculosis in a Neglected Area in the Amazonian Region Highlights the Urgent Need for Control Measures

Detection of non-tuberculosus mycobacteria (NTMs) in lung samples using 16S rRNA

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