Label-free quantitative proteomic analysis reveals muscle contraction and metabolism proteins linked to ultimate pH in bovine skeletal muscle

Poleti, Mirele Daiana; Moncau, Cristina Tschorny; Silva-Vignato, Bárbara; Rosa, Alessandra Fernandes; Jr, Adalfredo Rocha Lobo; Cataldi, Thaís Regiani; Negrão, João Alberto; Silva, Saulo da Luz e; Eler, Joanir Pereira; Balieiro, J. C. C.

doi:10.1016/j.meatsci.2018.06.041

Cited by 41 publications

(29 citation statements)

References 84 publications

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“…No statistical differences (p> 0.05) between cortisol values of pH<5.8 group (75.33 ng/mL) than those observed in the pH≥5.8 group (63.89 ng/mL) were observed. Similar results were reported by Poleti et al [ 26 ] who found cortisol levels of 61.6 and 70.2 ng/mL between high (pH≥6.0) and normal (pH<5.8) ultimate pH groups, respectively, in bovine Longissimus thoracis muscle. In our study, the percentages of animals with normal (25 to 45 ng/mL), high (>45 – 90 ng/mL), or extremely high (>90 ng/mL) [ 3 ] cortisol levels were 23.2% (36.1 ng/mL), 44.3% (63.0 ng/mL), and 22.7% (108.8 ng/mL), respectively.…”

Section: Resultssupporting

confidence: 90%

“…According to Abril et al [ 27 ], pH≥6.1 meat from male cattle had lower L *, a *, b *, C *, and h * values than pH<6.1 meat at the most measuring times; this is characteristic of the darker and redder color of DFD meats. Poleti et al [ 26 ] observed that meat with high pH≥6.0 had lower L *, a *, and b * values within all postmortem times, indicating that muscle with high pH was darker, less red, and less yellow. During rigor onset, the increase in the overlapping myofilaments and shortening of the sarcomere is associated with higher reflectance.…”

Section: Resultsmentioning

confidence: 99%

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Effect of stress during slaughter on carcass characteristics and meat quality in tropical beef cattle

Carrasco-García

Pardío-Sedas

León-Banda

et al. 2020

Asian-Australas J Anim Sci

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Effect of stress during slaughter on carcass characteristics and meat quality in tropical beef cattle

Carrasco-García

Pardío-Sedas

León-Banda

et al. 2020

Asian-Australas J Anim Sci

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“…[ 7 ] Moreover, FAO reported that a pHu higher than 6.2 indicates that the animal was stressed, injured, or diseased prior to slaughter. [ 9 ] Therefore, measurement of final pH during the conversion of muscle into meat is a highly important factor for meat industry [ 10 ] and it is used the most as routine practice to control the occurrence of defective meats. However, some studies have reported the main factors contributing to dark cutting meat and the controversial use of pHu to detect it.…”

Section: Introductionmentioning

confidence: 99%

“…[ 13 ] However, proteomic approaches addressing this problem in beef cattle are based on the study of total protein extract. [ 10,14,15 ] Alternatively, we have recently proposed an innovative proteomic approach based on liquid isoelectric focusing (OFFGEL) for the specific fractionation/enrichment of the sarcoplasmic fraction hyphenated with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) and LC‐MS analysis. [ 16 ] Although it is known that sarcoplasmic proteins (stress/defense proteins and metabolic enzymes) are the ones mainly related to stress, the number of studies reporting changes occurring on myofibrillar proteome are scarce and deserves further investigation.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Myofibrillar Proteome as a Way to Better Understand Differences in Bovine Meats Having Different Ultimate pH Values

et al. 2020

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Influence of ultimate pH (pHu) on the occurrence of defective meats known as dark, firm, and dry (DFD) meats has been studied through a proteomic approach at early post‐mortem times. The myofibrillar sub‐proteome of longissimus thoracis et lumborum muscle from twelve loin samples from Asturiana de los Valles x Friesian yearling bulls, previously classified into two groups of six samples according to their pH values (normal, pHu < 6.0 and high, pHu ≥ 6.0), is analyzed at 24 h post‐mortem. Fractionation/enrichment of muscle samples is carried out by combining OFFGEL fractionation in the pH range 4–7 followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) of the retrieved liquid fractions. Four protein bands satisfactorily discriminate between meat samples with normal and high pHu. These bands are quantified by image analysis, and further identified by liquid chromatography‐mass spectrometry as desmin, pyruvate kinase, myosin light chain, and myosin heavy chain‐1 and ‐2. Coupling OFFGEL and SDS‐PAGE separation with MS provides detailed and reproducible myofibrillar protein profiles enabling comparison among the sample groups assayed. This makes feasible to identify biomarkers capable to better understand pre‐slaughter stress condition susceptible to give DFD meats with high pHu values.

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“…About 987 results were revealed when a search was conducted in the PubMed (http://www.ncbi.nlm.gov/pubmed) database using "Proteomics, animal, and Brazil" terms. Approximately 68% of the articles were published in the last five years and include for instance topics such as beef quality and muscle function [30,31]. Pioneering reports also involved the proteome characterization of venomous secretions [32].…”

Section: Domestic Animal Proteomics In Brazil and Latin America In Recent Yearsmentioning

confidence: 99%

Domestic animal proteomics in the 21st century: A global retrospective and viewpoint analysis

Almeida

Ali

Ceciliani

et al. 2021

Journal of Proteomics

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Animal production and health are of significant economic importance, particularly regarding the world food supply. Animal and veterinary sciences have evolved immensely in the past six decades, particularly in genetics, nutrition, housing, management and health. To address major challenges such as those posed by climate change or metabolic disorders, it is of utmost importance to use state-of-the-art research tools. Proteomics and the other post-genomic tools (transcriptomics or metabolomics) are among them. Proteomics has experienced a considerable development over the last decades. This brought developments to different scientific fields. The use and adoption of proteomics tools in animal and veterinary sciences has some limitations (database availability or access to proteomics platforms and funding). As a result, proteomics' use by animal science researchers varies across the globe. In this viewpoint article, we focus on the developments of domestic animal proteomics over the last decade in different regions of the globe and how the researchers have coped with such challenges. In the second part of the article, we provide examples of funding, educational and laboratory establishment initiatives designed to foster the development of (animal-based) proteomics. International scientific collaboration is a definitive and key feature in the development and advancement of domestic animal proteomics. Significance: Animal production and health are very important for food supply worldwide particularly as a source of proteinaceous foods. Animal and veterinary sciences have evolved immensely in the last decades. In order to address the major contemporary challenges facing animal and veterinary sciences, it is of utmost importance to use state-of-the-art research tools such as Proteomics and other Omics. Herein, we focus on the major developments in domestic animal proteomics worldwide during the last decade and how different regions of the

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Label-free quantitative proteomic analysis reveals muscle contraction and metabolism proteins linked to ultimate pH in bovine skeletal muscle

Cited by 41 publications

References 84 publications

Effect of stress during slaughter on carcass characteristics and meat quality in tropical beef cattle

Effect of stress during slaughter on carcass characteristics and meat quality in tropical beef cattle

Characterization of the Myofibrillar Proteome as a Way to Better Understand Differences in Bovine Meats Having Different Ultimate pH Values

Domestic animal proteomics in the 21st century: A global retrospective and viewpoint analysis

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