Label-free quantitative proteomic analysis of drought stress-responsive late embryogenesis abundant proteins in the seedling leaves of two wheat (Triticum aestivum L.) genotypes

Li, Na; Zhang, Shuai; Liang, Yajun; Qi, Yuhong; Chen, Juan; Zhu, Weining; Zhang, Linsheng

doi:10.1016/j.jprot.2017.09.016

Cited by 35 publications

(18 citation statements)

References 95 publications

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“…An Em protein, H5, was over-represented by AF086. The Em gene of wheat encodes the first LEA protein group [99], playing a key role in environmental stress response [100]. They help other proteins to fold after denaturation during water stress [101].…”

Section: Proteins Involved In Regulation Processesmentioning

confidence: 99%

Analysis of durum wheat proteome changes under marine and fungal biostimulant treatments using large-scale quantitative proteomics: A useful dataset of durum wheat proteins

Pichereaux

Laurent

Gargaros

et al. 2019

Journal of Proteomics

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Section: Proteins Involved In Regulation Processesmentioning

confidence: 99%

Analysis of durum wheat proteome changes under marine and fungal biostimulant treatments using large-scale quantitative proteomics: A useful dataset of durum wheat proteins

Pichereaux

Laurent

Gargaros

et al. 2019

Journal of Proteomics

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“…Cellular enrichment of these hydrophilic proteins under water deficit conditions play a key role in maintaining cellular water status, and thus help to prevent the desiccation-induced aggregation of the water-soluble proteomes [42]. A recent study, on the leaf proteomes of drought treated wheat (Triticum aestivum L.), showed that the abundances of over half of the highly hydrophilic LEA proteins changed significantly after drought stress and they were involved in protection against drought [43]. One of the primary changes in response to drought is the generation of reactive oxygen species (ROS).…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomics of Root Apex and Root Elongation Zones Provides Insights into Molecular Mechanisms for Drought Stress and Recovery Adjustment in Switchgrass

Sangireddy

et al. 2020

Proteomes

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Switchgrass plants were grown in a Sandwich tube system to induce gradual drought stress by withholding watering. After 29 days, the leaf photosynthetic rate decreased significantly, compared to the control plants which were watered regularly. The drought-treated plants recovered to the same leaf water content after three days of re-watering. The root tip (1cm basal fragment, designated as RT1 hereafter) and the elongation/maturation zone (the next upper 1 cm tissue, designated as RT2 hereafter) tissues were collected at the 29th day of drought stress treatment, (named SDT for severe drought treated), after one (D1W) and three days (D3W) of re-watering. The tandem mass tags mass spectrometry-based quantitative proteomics analysis was performed to identify the proteomes, and drought-induced differentially accumulated proteins (DAPs). From RT1 tissues, 6156, 7687, and 7699 proteins were quantified, and 296, 535, and 384 DAPs were identified in the SDT, D1W, and D3W samples, respectively. From RT2 tissues, 7382, 7255, and 6883 proteins were quantified, and 393, 587, and 321 proteins DAPs were identified in the SDT, D1W, and D3W samples. Between RT1 and RT2 tissues, very few DAPs overlapped at SDT, but the number of such proteins increased during the recovery phase. A large number of hydrophilic proteins and stress-responsive proteins were induced during SDT and remained at a higher level during the recovery stages. A large number of DAPs in RT1 tissues maintained the same expression pattern throughout drought treatment and the recovery phases. The DAPs in RT1 tissues were classified in cell proliferation, mitotic cell division, and chromatin modification, and those in RT2 were placed in cell wall remodeling and cell expansion processes. This study provided information pertaining to root zone-specific proteome changes during drought and recover phases, which will allow us to select proteins (genes) as better defined targets for developing drought tolerant plants. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD017441.

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“…Increased proline accumulation correlates with higher RWC. A wheat genotype sensitive to drought exhibited reduced RWC at 30% of soil moisture, whereas RWC was not reduced in a drought tolerant genotype [ 45 ]. Similarly, a reduction in leaf water potential, stomatal conductance and transpiration rate, and an increase in leaf temperature and abscisic acid (ABA) level were observed in two genotypes of soybean under heat stress [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

et al. 2020

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Background Abiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level. Results In this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 °C) for 24 h. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free quantification proteomics. Across all samples, a total of 1878 protein groups were detected. Under heat stress, 45 protein groups in the empty vector plants were significantly altered (P < 0.05; |log2FC| > 2). Conversely, 105 protein groups were significantly altered when miR156OE alfalfa was subjected to heat stress, of which 91 were unique to miR156OE plants. The identified protein groups unique to miR156OE plants were related to diverse functions including metabolism, photosynthesis, stress-response and plant defenses. Furthermore, we identified transcription factors in miR156OE plants, which belonged to squamosa promoter binding-like protein, MYB, ethylene responsive factors, AP2 domain, ABA response element binding factor and bZIP families of transcription factors. Conclusions These results suggest a positive role for miR156 in heat stress response in alfalfa. They reveal a miR156-regulated network of mechanisms at the protein level to modulate heat responses in alfalfa.

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Label-free quantitative proteomic analysis of drought stress-responsive late embryogenesis abundant proteins in the seedling leaves of two wheat (Triticum aestivum L.) genotypes

Cited by 35 publications

References 95 publications

Analysis of durum wheat proteome changes under marine and fungal biostimulant treatments using large-scale quantitative proteomics: A useful dataset of durum wheat proteins

Analysis of durum wheat proteome changes under marine and fungal biostimulant treatments using large-scale quantitative proteomics: A useful dataset of durum wheat proteins

Comparative Proteomics of Root Apex and Root Elongation Zones Provides Insights into Molecular Mechanisms for Drought Stress and Recovery Adjustment in Switchgrass

Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

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