Label-Free Protease Sensors Based on Optical Microsphere Resonators

Hanumegowda, Niranjan M.; White, Ian M.; Oveys, Hesam; Fan, Xudong

doi:10.1166/sl.2005.044

Cited by 62 publications

(37 citation statements)

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“…Despite this, fused-silica microspheres have proved to be capable of mercury detection in water, with a detection limit of 50 ppb (w/w), which is comparable to results obtained using surface plasmon resonance [119]. Specific results concerning proteins like BSA [110], DNA [118] and protease [120], deposited on the surface of the microspheres, have shown that the LOD can be as low as 1-10 pg/mm 2 . Vollmer et al [118] also demonstrated that multiplexed DNA detection by using two microspheres was possible, and that a single nucleotide mismatch in an 11-mer 1 oligonucleotide could be discriminated.…”

Section: Biochemical Sensorsmentioning

confidence: 85%

Spherical whispering‐gallery‐mode microresonators

Chiasera

Dumeige

Féron

et al. 2010

Laser & Photonics Reviews

435

280

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International audienceWhispering-gallery modes (WGM) on a spherical surface were first described by Lord Rayleigh at the beginning of the last century, but only after the invention of laser did they start to have some scientific relevance and only during the last two decades there has been a substantial move towards real devices and practical applications. WGM resonators have peculiar properties, the most notable being the potential of having an ultrahigh quality factor Q, which makes them very appealing both as laser cavities and as extremely sensitive sensors. Among the different types of WGM resonators, the microspherical ones represent a very important category, due to their simplicity, easy fabrication, and very high quality. In this review we provide a description of their fundamental properties and we summarize recent works on their application as filters, sensors and lasers

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Section: Biochemical Sensorsmentioning

confidence: 85%

Spherical whispering‐gallery‐mode microresonators

Chiasera

Dumeige

Féron

et al. 2010

Laser & Photonics Reviews

435

280

View full text Add to dashboard Cite

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“…In these systems, the light is coupled to the WGM through evanescent waves from the side of an eroded optical fiber 35 or the end of an angle polished fiber. 38 Analytes measured include bovine serum albumin (BSA), 35 DNA, 34 bacteriorhodopsin, 32 trypsin (via removal of BSA), 15 mercury, 14 and thrombin. 40 For bulk index change, a model system of ethanol and water indicate a resolution of approximately 10 À7 refractive index units (RIU).…”

Section: Whispering Gallery Mode Sensorsmentioning

confidence: 99%

Whispering Gallery Mode Biosensors Consisting of Quantum Dot-Embedded Microspheres

2009

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New methods of biological analyte sensing are needed for development of miniature biosensors that are highly sensitive and require minimal sample preparation. One technique employs optical resonances, known as whispering gallery modes (WGMs), in spherical or cylindrical microstructures. The spectral positions of these resonant modes are very sensitive to the local refractive index and spectral shifts may be used to sense changes in the index. To excite these WGMs and enable remote excitation, quantum dots are embedded in polystyrene microspheres to serve as local light sources. Using a simple continuous wave excitation optical system, these sensors are demonstrated by monitoring the wavelength shift of multiple resonant modes as bulk index of refraction is changed in ethanol-water mixtures. The potential for targeted biosensing is explored through addition of a protein that adsorbs to the microsphere surface, thrombin, and one that does not, bovine serum albumin (BSA). The thrombin produced a spectral shift that was much larger than that due to the bulk index change. The BSA produced a significantly smaller shift that was slightly larger than the expected shift due to bulk index change. Most likely due to the thin, high index layer of quantum dots, microsensor response in all cases demonstrated increased sensitivity over theoretical predictions.

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“…3, typical optical detection methods comprise the direct detection by monitoring the light properties including fluorescence, 74,[81][82][83] absorbance, [84][85][86] and luminescence-based [87][88][89] methods, and the light property modulation detections such as surface plasmon 78 and optical waveguides. 79,132 Photonic crystals, optical cavity structures, and several optical techniques have also been reported to be integrated with the microfluidic system for sensing purposes, using one-and two-dimensional surface PCs (guided mode resonance filters), [133][134][135][136] , or three-dimensional photonic crystals, 137,138 optical cavities, 139,140 whispering gallery mode resonators, [141][142][143][144][145][146][147] and optical tweezers for cell related monitoring or fluidic rheological measurements [148][149][150] (Fig. 4).…”

Section: Detection Methodsmentioning

confidence: 99%