Label-Free Detection of DNA Hybridization Using Pyrene-Functionalized Single-Walled Carbon Nanotubes: Effect of Chemical Structures of Pyrene Molecules on DNA Sensing Performance

Baek, Youn-Kyoung; Jung, Dae-Sung; Yoo, Seung Min; Shin, Sunhaera; Kim, Ju-Hyun; Jeon, Hwan‐Jin; Choi, Yang‐Kyu; Lee, Sang Yup; Jung, Hee‐Tae

doi:10.1166/jnn.2011.3663

Cited by 15 publications

(8 citation statements)

References 12 publications

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“…The linker molecules were also characterised using absorbance spectra, which could easily resolve a concentration level of 0.2 µg/mL, as shown in Figure 7. The data show clear triple peaks in absorbance due to the pyrene moiety that is central to the Pyr-NHS ester linker molecules, also observed by, Baek et al (2011) after functionalising carbon nanotubes for electrical detection of DNA hybridisation. The data in Figure 7 also show a repeat measurement (off-set for clarity) of the absorbance spectra over the 220-400 nm wavelength range, demonstrating excellent repeatability of the measurements.…”

Section: Functionalisation Of Gfetssupporting

confidence: 56%

Graphene FET Sensors for Alzheimer’s Disease Protein Biomarker Clusterin Detection

Bungon

Haslam

Damiati

et al. 2021

Front. Mol. Biosci.

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We report on the fabrication and characterisation of graphene field-effect transistor (GFET) biosensors for the detection of Clusterin, a prominent protein biomarker of Alzheimer’s disease (AD). The GFET sensors were fabricated on Si/SiO2 substrate using photolithographic patterning and metal lift-off techniques with evaporated chromium and sputtered gold contacts. Raman Spectroscopy was performed on the devices to determine the quality of the graphene. The GFETs were annealed to improve their performance before the channels were functionalized by immobilising the graphene surface with linker molecules and anti-Clusterin antibodies. Concentration of linker molecules was also independently verified by absorption spectroscopy using the highly collimated micro-beam light of Diamond B23 beamline. The detection was achieved through the binding reaction between the antibody and varying concentrations of Clusterin antigen from 1 to 100 pg/mL, as well as specificity tests using human chorionic gonadotropin (hCG), a glycoprotein risk biomarker of certain cancers. The GFETs were characterized using direct current (DC) 4-probe electrical resistance (4-PER) measurements, which demonstrated a limit of detection of the biosensors to be ∼ 300 fg/mL (4 fM). Comparison with back-gated Dirac voltage shifts with varying concentration of Clusterin show 4-PER measurements to be more accurate, at present, and point to a requirement for further optimisation of the fabrication processes for our next generation of GFET sensors. Thus, we have successfully fabricated a promising set of GFET biosensors for the detection of Clusterin protein biomarker. The developed GFET biosensors are entirely generic and also have the potential to be applied to a variety of other disease detection applications such as Parkinson’s, cancer, and cardiovascular.

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Section: Functionalisation Of Gfetssupporting

confidence: 56%

Graphene FET Sensors for Alzheimer’s Disease Protein Biomarker Clusterin Detection

Bungon

Haslam

Damiati

et al. 2021

Front. Mol. Biosci.

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“…They confirmed the adhesion of the high aromatic system on the graphene surface by electrochemical measurement, and confirmed by controlled experiments that the irreversible adhesion of the high aromatic system on the graphene surface was formed by the non-covalent manner of the πstacking of the aromatic ring. Both Hongjie Dai et al [49] and Baek et al [50] also found the similar strong interact on the surface of carbon nanotubes. Since the product has more benzene rings in the NDI and the branch, the benzene ring can interact with the carbon six-membered ring of the carbon material to attach the product to the EG (SWNT).…”

Section: Uv-vis @ CDmentioning

confidence: 74%

Self-assembly and cell imaging behaviors of Tripeptide-naphthalenediimide and interactions with carbon nano-materials

Xiao¹,

Wang²,

Zhang³

et al. 2019

Preprint

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Compared with other self-assembling molecule, peptide, especially unprotected peptide have better biocompatibility and are more acceptable for food science, cosmetics and biopharmaceuticals. However, the regulation of the microstructure formed by peptide self-assembled supramolecular remains a major problem. Herein we have designed three amphiphilic supramolecular thCompared with other self-assembling molecule, peptide, especially unprotected peptide have better biocompatibility and are more acceptable for food science, cosmetics and biopharmaceuticals. However, the regulation of the microstructure formed by peptide self-assembled supramolecular remains a major problem. Herein we have designed three amphiphilic supramolecular that can self-assemble to form specific structures. The amphiphilic supramolecular use 1,4,5,8-naphthalenetetracarboxylic anhydride as the self-assembling framework to form some specific nanostructure by the regulation of the tripeptide molecule attached to both ends of the naphthalene diimide molecules. As designed, we have observed nanostructures such as spheres, squares, and needles formed under acidic or basic condition by electron microscopy images. And we attached the molecule to the carbon six-membered ring structure of carbon nanotubes and graphene, which provides a method for improving the dispersibility of carbon nanotubes and graphene. The peptide-based molecular designs enforce intimate π-π communication and hydrogen bonding within the aggregates after self-assembly, making these nanostructures attractive for optical or electronic applications in biological environments.at can self-assemble to form specific structures. The amphiphilic supramolecular use 1,4,5,8-naphthalenetetracarboxylic anhydride as the self-assembling framework to form some specific nanostructure by the regulation of the tripeptide molecule attached to both ends of the naphthalene diimide molecules. As designed, we have observed nanostructures such as spheres, squares, and needles formed under acidic or basic condition by electron microscopy images. And we attached the molecule to the carbon six-membered ring structure of carbon nanotubes and graphene, which provides a method for improving the dispersibility of carbon nanotubes and graphene. The peptide-based molecular designs enforce intimate π-π communication and hydrogen bonding within the aggregates after self-assembly, making these nanostructures attractive for optical or electronic applications in biological environments.

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“…The biosensor described efficiently discriminated a complementary and a mismatch-containing targets. Baek et al studied the effect of the structure of the pyrene anchor on the biosensor performance [ 258 ]. Three types of pyrene derivatives (1-pyrenebutanoic acid, 1-pyrene carboxaldehyde, and 1-pyrenyl methylamine) were adsorbed on SWCNT films and reacted with oligonucleotides in specific conditions (carboxylic group activation, Schiff’s base reaction, followed by the reduction of amide, and UV-crosslinking, respectively) ( Figure 39 ).…”

Section: Supramolecular Assembliesmentioning

confidence: 99%

Recent Advances in Nucleic Acid Targeting Probes and Supramolecular Constructs Based on Pyrene-Modified Oligonucleotides

et al. 2017

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In this review, we summarize the recent advances in the use of pyrene-modified oligonucleotides as a platform for functional nucleic acid-based constructs. Pyrene is of special interest for the development of nucleic acid-based tools due to its unique fluorescent properties (sensitivity of fluorescence to the microenvironment, ability to form excimers and exciplexes, long fluorescence lifetime, high quantum yield), ability to intercalate into the nucleic acid duplex, to act as a π-π-stacking (including anchoring) moiety, and others. These properties of pyrene have been used to construct novel sensitive fluorescent probes for the sequence-specific detection of nucleic acids and the discrimination of single nucleotide polymorphisms (SNPs), aptamer-based biosensors, agents for binding of double-stranded DNAs, and building blocks for supramolecular complexes. Special attention is paid to the influence of the design of pyrene-modified oligonucleotides on their properties, i.e., the structure-function relationships. The perspectives for the applications of pyrene-modified oligonucleotides in biomolecular studies, diagnostics, and nanotechnology are discussed.

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Label-Free Detection of DNA Hybridization Using Pyrene-Functionalized Single-Walled Carbon Nanotubes: Effect of Chemical Structures of Pyrene Molecules on DNA Sensing Performance

Cited by 15 publications

References 12 publications

Graphene FET Sensors for Alzheimer’s Disease Protein Biomarker Clusterin Detection

Graphene FET Sensors for Alzheimer’s Disease Protein Biomarker Clusterin Detection

Self-assembly and cell imaging behaviors of Tripeptide-naphthalenediimide and interactions with carbon nano-materials

Recent Advances in Nucleic Acid Targeting Probes and Supramolecular Constructs Based on Pyrene-Modified Oligonucleotides

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