Label-Free Bimetallic In Situ-Grown 3D Nickel-Foam-Supported NH₂-MIL-88B(Fe₂Co)-MOF-based Impedimetric Immunosensor for the Detection of Cardiac Troponin I

Abstract

In this study, a simple and competent metal–organic framework (MOF)-based nickel foam (NF)-supported three-dimensional (3D) immunosensor (Ab-NH2-MIL-88B­(Fe2Co)-MOF/NF) was constructed and utilized for the specific recognition of the biomarker cardiac troponin (I) (cTnI). In the present work, biosensor fabrication was progressed through the modification of the NF substrate with the MOF material (NH2-MIL-88B­(Fe2Co)-MOF) to enable an amine-functionalized electrode. This amine-functionalized NF electrodes (NH2-MIL-88B­(Fe2Co)-MOF/NF) were then biointerfaced with anti-cTnI antibodies, which ended up as Ab-NH2-MIL-88B­(Fe2Co)-MOF/NF electrodes. Analytical executions of the constructed bioelectrode were investigated for the quantitative analysis of cTnI in both buffered and serum solutions. Then, the electrochemical studies were carried out using the electrochemical impedance spectroscopy (EIS) method by monitoring changes concerning the charge transfer resistance (R ct) characteristics. The limit of detection (LOD) of the Ab-NH2-MIL-88B­(Fe2Co)-MOF/NF immunosensor was achieved to be 13 fg/mL with great specificity. This kind of immunosensor imparts a new platform for the construction and application of MOF-hybrid 3D electrode materials with enhanced electrochemical behavior in cTnI sensing for the first time.

Fabrication of 3D Amino-Functionalized Metal–Organic Framework on Porous Nickel Foam Skeleton to Combinate Follicle Stimulating Hormone Antibody for Specific Recognition of Follicle-Stimulating Hormone

“…The as-prepared materials showed main diffraction peaks at 9.39°and 17.95°which are similar to that of the reported diffraction patterns of the MIL 101 series. 46,47 The XRD patterns of the synthesized H 2 N−Fe-MIL-101 samples exhibited strong peaks fairly at high intensity and flat background, indicating high crystallinity of the materials. In addition, no other peaks were recorded for the as-obtained sample evidencing the high phase purity of H 2 N− Fe-MIL-101.…”

Electrochemical Impedance Spectroscopy (EIS) Based Label-Free Immunosensors

“…Successful target detection includes a number of cancer biomarkers [238][239][240][241][242] and Alzheimer disease biomarkers, 91,243 and cardiovascular disease biomarkers. [244][245][246][247][248] Diagnostics are not limited to diseases in human, pen-side devices are also desirable for animal health monitoring. Work…”

A Comprehensive Overview on Advanced Sensing Applications of Functional Metal Organic Frameworks (MOFs)

“…With respect to the changes related to the charge transfer resistance characteristics, the EIS method was exploited for electrochemical studies. [104] Zhou et al engineered a sensitive EIS aptasensor for proteins using carcinoembryonic antigen (CEA) as an analyte model. They developed a peroxidase-analogue nanocomposite consisting of Cu-MOF functionalised with Pt nanoparticles, hemin, CEA aptamer and GOx (glucose oxidase).…”

“…These amine‐functionalised NF electrodes were bio‐interfaces with anti‐cTnI antibodies, which ended up as Ab‐NH 2 ‐MIL‐88B(Fe 2 Co)‐MOF/NF electrodes. With respect to the changes related to the charge transfer resistance characteristics, the EIS method was exploited for electrochemical studies [104] …”