Label-Free and Real-Time Detection of Avian Influenza Using Nanowire Field Effect Transistors

Ahn, Jae Hyuk; Im, Maesoon; Park, Tae Jung; Lee, Sang Yup; Choi, Yang Kyu

doi:10.1166/jbn.2015.1501

Cited by 8 publications

(9 citation statements)

References 11 publications

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“…163 In addition, several advanced or modified assays and platforms for detecting H7N9 AIVs are under investigation, such as modified surface plasmon resonance, 39 enzyme-induced metallization, 164 reverse transcription-loop-mediated isothermal amplification assay, 165 and chip-based biosensors. 166 This study has some limitations. We selected articles written in English only; in addition, we excluded small cohort studies and clinical trials and focused mainly on study results in human cases of AIV infection.…”

Section: Discussionmentioning

confidence: 91%

Virus-induced pathogenesis, vaccine development, and diagnosis of novel H7N9 avian influenza A virus in humans: a systemic literature review

Mrc

et al. 2019

J Int Med Res

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H7N9 avian influenza virus (AIV) caused human infections in 2013 in China. Phylogenetic analyses indicate that H7N9 AIV is a novel reassortant strain with pandemic potential. We conducted a systemic review regarding virus-induced pathogenesis, vaccine development, and diagnosis of H7N9 AIV infection in humans. We followed PRISMA guidelines and searched PubMed, Web of Science, and Google Scholar to identify relevant articles published between January 2013 and December 2018. Pathogenesis data indicated that H7N9 AIV belongs to low pathogenic avian influenza, which is mostly asymptomatic in avian species; however, H7N9 induces high mortality in humans. Sporadic human infections have recently been reported, caused by highly pathogenic avian influenza viruses detected in poultry. H7N9 AIVs resistant to adamantine and oseltamivir cause severe human infection by rapidly inducing progressive acute community-acquired pneumonia, multiorgan dysfunction, and cytokine dysregulation; however, mechanisms via which the virus induces severe

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Section: Discussionmentioning

confidence: 91%

Virus-induced pathogenesis, vaccine development, and diagnosis of novel H7N9 avian influenza A virus in humans: a systemic literature review

Mrc

et al. 2019

J Int Med Res

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“…The signal (20–140 mV) and limit of detection (∼5.8 pM) of our sensor were comparable to or higher than those of other aptamer-based FET sensors. ,,− However, direct comparisons could not be made due to different aptamer and target biomolecules. Various FET-based approaches have been proposed for AIV detection, − ,− but this work is the first study to demonstrate the applicability of DNA aptamer as a bioreceptor, as summarized in Table . The sensitivity and dynamic range of our sensor were compared to those of the previously reported FET-based sensors for AIV detection.…”

Section: Resultsmentioning

confidence: 99%

“…These methods are generally effective but require time-consuming procedures, bulky and expensive equipment, and trained technicians, which limits their applications in on-site use. As an alternative, label-free electrical detection of H5N1 AIV based on field-effect transistors (FETs) is suitable for portable applications in field tests because it provides rapid signal response, hand-held readout system, low cost, and easy operation. − An FET is greatly advantageous for a transducer because it offers superior limit of detection, which is as low as the picomolar level, analysis of a small sample volume, and scalable manufacturing process. − …”

mentioning

confidence: 99%

Aptamer-Based Field-Effect Transistor for Detection of Avian Influenza Virus in Chicken Serum

et al. 2020

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Early diagnosis of the highly pathogenic H5N1 avian influenza virus (AIV) is significant for preventing and controlling a global pandemic. However, there is no existing electrical biosensor for detecting biomarkers for AIV in clinically relevant samples such as chicken serum. Herein, we report the first use of an aptamer-functionalized field-effect transistor (FET) as a label-free sensor for AIV detection in chicken serum. A DNA aptamer is employed as a sensitive and selective receptor for hemagglutinin (HA) protein, which is a biomarker for AIVs. This aptamer is immobilized on a gold microelectrode that is connected to the gate of a reusable FET transducer. The specific binding of the target protein results in a change in the surface potential, which generates a signal response of the FET transducer. We hypothesize that a conformational change in the DNA aptamer upon specific binding of HA protein may alter the surface potential. The signal of the aptamer-based FET biosensor increased linearly with the increase in the logarithm of HA protein concentration in a dynamic range of 10 pM to 10 nM with a detection limit of 5.9 pM. The selectivity of the biosensor for HA protein was confirmed by employing relevant interfering proteins. The proposed biosensor was successfully applied to the selective detection of HA protein in a chicken serum sample. Owing to its simple and low-cost architecture, portability, and sensitivity, the aptamer-based FET biosensor has potential as a point-of-care diagnosis of H5N1 AIVs in clinical samples.

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“…Immuno-FEDs for the detection of antibodies against viruses have been rarely studied (mostly as proof-of-concept experiments). For example, a nanogap FET (Gu et al, 2009), an underlap channel-embedded FET (Lee et al, 2010), and a SiNW-FET (Ahn et al, 2015) were realized to detect specific antibodies directed against avian influenza viruses. Moreover, the multiplexed detection of antibodies against avian influenza and human immunodeficiency viruses (HIV) by means of an underlap-embedded SiNW-FET is demonstrated (Kim et al, 2014).…”

Section: Detection Of Host Antibodiesmentioning

confidence: 99%

Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers

Poghossian¹,

Jablonski

Molinnus

et al. 2020

Front. Plant Sci.

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Coronavirus disease 2019 (COVID-19) is a novel human infectious disease provoked by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Currently, no specific vaccines or drugs against COVID-19 are available. Therefore, early diagnosis and treatment are essential in order to slow the virus spread and to contain the disease outbreak. Hence, new diagnostic tests and devices for virus detection in clinical samples that are faster, more accurate and reliable, easier and cost-efficient than existing ones are needed. Due to the small sizes, fast response time, label-free operation without the need for expensive and time-consuming labeling steps, the possibility of real-time and multiplexed measurements, robustness and portability (point-of-care and on-site testing), biosensors based on semiconductor field-effect devices (FEDs) are one of the most attractive platforms for an electrical detection of charged biomolecules and bioparticles by their intrinsic charge. In this review, recent advances and key developments in the field of label-free detection of viruses (including plant viruses) with various types of FEDs are presented. In recent years, however, certain plant viruses have also attracted additional interest for biosensor layouts: Their repetitive protein subunits arranged at nanometric spacing can be employed for coupling functional molecules. If used as adapters on sensor chip surfaces, they allow an efficient immobilization of analyte-specific recognition and detector elements such as antibodies and enzymes at highest surface densities. The display on plant viral bionanoparticles may also lead to long-time stabilization of sensor molecules upon repeated uses and has the potential to increase sensor performance substantially, compared to conventional layouts. This has been demonstrated in different proof-of-concept biosensor devices. Therefore, richly available plant viral particles, non-pathogenic for animals or humans, might gain novel importance if applied in receptor layers of FEDs. These perspectives are explained and discussed with regard to future detection strategies for COVID-19 and related viral diseases.

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Label-Free and Real-Time Detection of Avian Influenza Using Nanowire Field Effect Transistors

Cited by 8 publications

References 11 publications

Virus-induced pathogenesis, vaccine development, and diagnosis of novel H7N9 avian influenza A virus in humans: a systemic literature review

Virus-induced pathogenesis, vaccine development, and diagnosis of novel H7N9 avian influenza A virus in humans: a systemic literature review

Aptamer-Based Field-Effect Transistor for Detection of Avian Influenza Virus in Chicken Serum

Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers

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