Lab-on-a-chip modules for detection of highly pathogenic bacteria: from sample preparation to detection

Julich, Sandra; Kopinč, Rok; Hlawatsch, Nadine; Moche, Christian; Lapanje, Aleš; Gärtner, Claudia; Tomaso, Herbert

doi:10.1117/12.2049859

Cited by 2 publications

(3 citation statements)

References 26 publications

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“…Microfluidics and lab-on-a-chip (LOC) approaches have the potential to alleviate many of these concerns while retaining the advantages of traditional FC. These approaches can also incorporate many different types of sensing formats, making them quite versatile. − Such microfluidic devices are part of the larger microelectromechanical systems (MEMS) family. Typical versions are fabricated using photolithography to etch the channels into polydimethylsiloxane (PDMS), and these are attached to a glass base, for example. , Of course, there are many other materials iterations, fabrication techniques, and final functional formats.…”

Section: Spectroscopic Sensorsmentioning

confidence: 99%

“…Microfluidic/MEMS platforms have been used to detect many different biothreat agents using a wide variety of experimental setups . Due to full sample processing integration, they have been explored for on-site detection of foodborne pathogens, , including, E. coli and Salmonella . − Such devices have also been used for the capture of both sputum and airborne bacteria, as was demonstrated in the case of M. tuberculosis . , Other efforts have used MEMS in the detection of B. anthracis Sterne spore germination or the detection of PCR amplicons targeting this same pathogen’s genetic material …”

Section: Spectroscopic Sensorsmentioning

confidence: 99%

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Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

et al. 2018

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Although a fundamental understanding of the pathogenicity of most biothreat agents has been elucidated and available treatments have increased substantially over the past decades, they still represent a significant public health threat in this age of (bio)terrorism, indiscriminate warfare, pollution, climate change, unchecked population growth, and globalization. The key step to almost all prevention, protection, prophylaxis, post-exposure treatment, and mitigation of any bioagent is early detection. Here, we review available methods for detecting bioagents including pathogenic bacteria and viruses along with their toxins. An introduction placing this subject in the historical context of previous naturally occurring outbreaks and efforts to weaponize selected agents is first provided along with definitions and relevant considerations. An overview of the detection technologies that find use in this endeavor along with how they provide data or transduce signal within a sensing configuration follows. Current "gold" standards for biothreat detection/diagnostics along with a listing of relevant FDA approved in vitro diagnostic devices is then discussed to provide an overview of the current state of the art. Given the 2014 outbreak of Ebola virus in Western Africa and the recent 2016 spread of Zika virus in the Americas, discussion of what constitutes a public health emergency and how new in vitro diagnostic devices are authorized for emergency use in the U.S. are also included. The majority of the Review is then subdivided around the sensing of bacterial, viral, and toxin biothreats with each including an overview of the major agents in that class, a detailed cross-section of different sensing methods in development based on assay format or analytical technique, and some discussion of related microfluidic lab-on-a-chip/point-of-care devices. Finally, an outlook is given on how this field will develop from the perspective of the biosensing technology itself and the new emerging threats they may face.

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Section: Spectroscopic Sensorsmentioning

confidence: 99%

Section: Spectroscopic Sensorsmentioning

confidence: 99%

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

et al. 2018

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“…We evaluated the microfluidic fluidized bed for performing individual steps of the magnetic bead PLP/C2CA protocol for DNA analysis (Fig. 1b), and compared it to ChipGenie® edition P (Fig 3), a commercially available benchtop solution for magnetic bead handling (Holubova et al 2014;Julich et al 2016;Julich et al 2014;Kucerova et al 2014). This instrument is designed with a holder for microscope slide format microfluidic chips, possesses a heating element and a magnet placed underneath the chip, and can perform magnetic bead actuation for mixing and incubation by mechanical movement of the magnet along the length of the microchannel.…”

Section: Efficiencies Of Individual Plp/c2ca Steps Using the Magneticmentioning

confidence: 99%

Microfluidic magnetic fluidized bed for DNA analysis in continuous flow mode

Hernández-Neuta

Pereiro

Ahlford

et al. 2018

Biosensors and Bioelectronics

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Magnetic solid phase substrates for biomolecule manipulation have become a valuable tool for simplification and automation of molecular biology protocols. However, the handling of magnetic particles inside microfluidic chips for miniaturized assays is often challenging due to inefficient mixing, aggregation, and the advanced instrumentation required for effective actuation. Here, we describe the use of a microfluidic magnetic fluidized bed approach that enables dynamic, highly efficient and simplified magnetic bead actuation for DNA analysis in a continuous flow platform with minimal technical requirements. We evaluate the performance of this approach by testing the efficiency of individual steps of a DNA assay based on padlock probes and rolling circle amplification. This assay comprises common nucleic acid analysis principles, such as hybridization, ligation, amplification and restriction digestion. We obtained efficiencies of up to 90% for these reactions with high throughput processing up to 120μL of DNA dilution at flow rates ranging from 1 to 5μL/min without compromising performance. The fluidized bed was 20-50% more efficient than a commercially available solution for microfluidic manipulation of magnetic beads. Moreover, to demonstrate the potential of this approach for integration into micro-total analysis systems, we optimized the production of a low-cost polymer based microarray and tested its analytical performance for integrated single-molecule digital read-out. Finally, we provide the proof-of-concept for a single-chamber microfluidic chip that combines the fluidized bed with the polymer microarray for a highly simplified and integrated magnetic bead-based DNA analyzer, with potential applications in diagnostics.

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Lab-on-a-chip modules for detection of highly pathogenic bacteria: from sample preparation to detection

Cited by 2 publications

References 26 publications

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

Microfluidic magnetic fluidized bed for DNA analysis in continuous flow mode

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