La résistance aux traitements antiangiogéniques

Gu, Yuchen; Lű, He; Boisson-Vidal, Catherine; Li, Hong; Bousquet, Guilhem; Janin, Anne; Benedetto, Mélanie Di

doi:10.1051/medsci/20163204015

Cited by 3 publications

(1 citation statement)

References 47 publications

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“…[16][17][18] This was not confirmed in our "real-life" cohort, possibly due to another chemotherapy resistance mechanism: overexpression of p-glycoprotein. 19,20 This overexpression decreases SN-38 (irinotecan metabolite) concentration in cells. 21 In murine models, bevacizumab can reduce p-glycoprotein activity and thus increase SN-38 concentration and chemotherapy activity.…”

Section: Discussionmentioning

confidence: 99%

FOLFIRI plus BEvacizumab or aFLIbercept after FOLFOX‐bevacizumab failure for COlorectal cancer (BEFLICO): An AGEO multicenter study

Torregrosa

Pernot

Vaflard

et al. 2022

Intl Journal of Cancer

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Section: Discussionmentioning

confidence: 99%

FOLFIRI plus BEvacizumab or aFLIbercept after FOLFOX‐bevacizumab failure for COlorectal cancer (BEFLICO): An AGEO multicenter study

Torregrosa

Pernot

Vaflard

et al. 2022

Intl Journal of Cancer

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Un nouveau rôle non métabolique de la glutamine synthétase au cours de l’angiogenèse

Dubois¹,

Eelen

Carmeliet

2019

Med Sci (Paris)

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Drug resistance‑related sunitinib sequestration in autophagolysosomes of endothelial cells

Huang

Shen

et al. 2019

Int J Oncol

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Our previous study demonstrated that the tyrosine kinase receptor inhibitor sunitinib induces acquired drug resistance in endothelial cells. The present study explored the role of lysosomal sequestration of sunitinib in the acquisition of drug resistance in human microcapillary endothelial HMEC-1 cells. Resistance was induced by escalating concentrations of sunitinib and a shift in IC50 from 12.8 to >20 µM was detected. The results of time-lapse fluorescence microscopy illustrated an instantaneous emergence of fluorescent vesicles in living cells once sunitinib was added. Most of these vesicles emerged in the juxtanuclear area, and exhibited the characteristics of growing autophagosomes and lysosomes. The vesicles were identified as autophagosomes and lysosomes because they co-located with the lysosomal tracers Lyso-ER and Lyso-NIR, and the protein markers lysosomal-associated membrane protein 1 (LAMP-1) and microtubule-associated protein 1A/1B-light chain 3 (LC3). The results of western blotting demonstrated that sunitinib induced upregulation of LAMP-1 and LC3-II, and downregulation of sequestosome 1/p62, indicating the activation of autophagy. Bafilomycin A1, which suppresses lysosomal acidification, completely blocked sunitinib sequestration; however, chloroquine, which blocks lysosomal fusion with autophagosomes, exhibited no effect. Notably, bafilomycin A1 and chloroquine significantly counterbalanced HMEC-1 drug-resistance. These results provided evidence for autophagy-flux-associated sunitinib lysosomal sequestration in endothelial cells, leading to isolation of the drug from the cytoplasm; a key process involved in the development of drug resistance during antiangiogenic therapy. These data supported the notion that inhibiting autophagy may be a potential strategy to prevent drug sequestration and resistance to antiangiogenic therapy.

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La résistance aux traitements antiangiogéniques

Cited by 3 publications

References 47 publications

FOLFIRI plus BEvacizumab or aFLIbercept after FOLFOX‐bevacizumab failure for COlorectal cancer (BEFLICO): An AGEO multicenter study

FOLFIRI plus BEvacizumab or aFLIbercept after FOLFOX‐bevacizumab failure for COlorectal cancer (BEFLICO): An AGEO multicenter study

Un nouveau rôle non métabolique de la glutamine synthétase au cours de l’angiogenèse

Drug resistance‑related sunitinib sequestration in autophagolysosomes of endothelial cells

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