La enfermedad diarreica aguda: un reto para la salud pública en Colombia

Cáceres, Diana Carolina; Estrada, Eduardo Cordero; DeAntonio, Rodrigo; Peláez, Dioselina

doi:10.1590/s1020-49892005000100002

Cited by 16 publications

(13 citation statements)

References 20 publications

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“…Differences among socioeconomic groups are an important factor in the genesis of diseases related to the widespread microbiological contamination of the environment [32]. Several studies in Latin America have shown that adequate hygiene practices and good sanitary conditions decrease the levels of contamination significantly [14, 15, 33]. In our study, due to the sample size, no significant differences among strata were shown in the STEC contamination level of ground beef.…”

Section: Discussioncontrasting

confidence: 49%

Characterization of Shiga Toxin-ProducingEscherichia coliIsolated from Ground Beef Collected in Different Socioeconomic Strata Markets in Buenos Aires, Argentina

Llorente

Barnech

Irino

et al. 2014

BioMed Research International

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Consumption of raw/undercooked ground beef is the most common route of transmission of Shiga toxin-producing E. coli (STEC). The aim of the study was to determine the STEC contamination level of the ground beef samples collected in 36 markets of different socioeconomic strata in Buenos Aires, Argentina, and the characterization of the isolated strains. Ninety-one out of 252 (36.1%) samples were stx+. Fifty-seven STEC strains were recovered. Eleven STEC strains belonged to O157 serogroup, and 46 to non-O157 serogroups. Virulence markers of the 57 STEC were stx1, 5.3% (3/57); stx2, 86.0% (49/57); stx1/stx2, 8.8% (5/57); ehxA, 61.4% (35/57); eae, 26.3% (15/57); saa, 24.6% (14/57). Shiga toxin subtypes were stx2, 31.5% (17/54); stx2c-vhb, 24.1% (13/54); stx2c-vha, 20.4% (11/54); stx2/stx2c-vha, 14.8% (8/54); stx2/stx2c-vhb, 5.6% (3/54); stx2c-vha/vhb, 3.7% (2/54). Serotypes O178:H19 and O157:H7 were prevalent. Contamination rate of STEC in all strata was high, and the highest O157 contamination was observed at low strata at several sampling rounds. Persistence of STEC was not detected. Sixteen strains (28.1%) were resistant to ampicillin, streptomycin, amikacin, or tetracycline. The STEC contamination level of ground beef could vary according to the sociocultural characteristics of the population.

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Section: Discussioncontrasting

confidence: 49%

Characterization of Shiga Toxin-ProducingEscherichia coliIsolated from Ground Beef Collected in Different Socioeconomic Strata Markets in Buenos Aires, Argentina

Llorente

Barnech

Irino

et al. 2014

BioMed Research International

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“…Genetic variability is, thus, increased and, conse quently, nontypeable strains appear ( 12,29,51). Fischer et al ( 19) found that in 20% of nontypeable P strains, the VP4 gene showed point mutations in the P [8] primer when it was amplified and sequenced. Iturriza-Gomara et al (34) found that the recognition site for the P [8] primers shows variability and, therefore, the previously designed primers (22) do not amplify these genes.…”

Section: Discussionmentioning

confidence: 99%

“…Fischer et al ( 19) found that in 20% of nontypeable P strains, the VP4 gene showed point mutations in the P [8] primer when it was amplified and sequenced. Iturriza-Gomara et al (34) found that the recognition site for the P [8] primers shows variability and, therefore, the previously designed primers (22) do not amplify these genes. Consequently, we suggest that nontypeable strains must be sequenced to achieve their identification.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of G2P [4] rotavirus genotype in foods might be caused by a change in the distribution of the various rotavirus genotypes that cause pediatric gastroen- teritis. This has occurred worldwide: in Ecuador, the new genotype G9 P [8] was found in 62% of clinical samples in 2007 (76); in Korea, G4P [6] was found in 26.4% of samples (35,36); and, in the United States, G1P [4] was identified in 41% of samples ( 1).…”

Section: Discussionmentioning

confidence: 99%

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Rotavirus G2P[4] Detection in Fresh Vegetables and Oysters in Mexico City

Quiroz-Santiago

Vázquez-Salinas

Natividad-Bonifacio

et al. 2014

Journal of Food Protection

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Rotaviruses are the principal cause of dehydration caused by diarrhea in children younger than 2 years of age. Although these viral infections have mainly been associated with ingestion of fecally contaminated food and water, few studies have addressed the presence of the virus in food that is consumed raw or slightly cooked. In this work, 30 oyster samples and 33 vegetable samples were examined for the presence of rotavirus genotypes to evaluate their potential to produce gastrointestinal infections. The rotaviruses were identified by reverse transcriptase PCR amplification of the VP7 gene. G and P genotyping was also performed by reverse transcriptase PCR, with a detection sensitivity of up to 15 PFU/ml. Rotaviruses were found in 17 (26.9%) of 63 samples (10 oysters and 7 vegetables). The G2 genotype was found in 11 (64.7%) of 17 of the rotavirus strains, and 16 (94.1%) of 17 had the P[4] genotype. The combined genotypes found most frequently were G2P[4] (10 [58.82%] of 17), GNTP[4] (6 [35.29%] of 17), and G2P[NT] (1 [5.8%] of 17).

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“…This city has a population of about 525,000 and is located on a plateau of the Andes at 959 meters above sea level, with an average temperature of 23°C and annual rainfall of 1041 mm. The city has over 90% coverage for basic utilities including those for water, electricity, telephone, gas, and garbage collection with a frequency of three times a week [13]. The coverage of pediatric hospital beds in this region is 0.48 per 1000 population for children under 12 years of age.…”

Section: Methodsmentioning

confidence: 99%

Case-Control Pilot Study on Acute Diarrheal Disease in a Geographically Defined Pediatric Population in a Middle Income Country

Farfán-García

Zhang

Imdad

et al. 2017

International Journal of Pediatrics

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Introduction Acute diarrheal disease (ADD) is a common cause of morbidity and mortality in children under 5 years of age. Understanding of the etiology of ADD is lacking in most low and middle income countries because reference laboratories detect limited number of pathogens. The objective of this study was to determine the feasibility to conduct a comprehensive case-control study to survey diarrheal pathogens among children with and without moderate-to-severe ADD. Materials and Methods Microbiology and molecular-based techniques were used to detect viral, bacterial, and parasitic enteropathogens. The study was conducted in Bucaramanga, Colombia, after Institutional Review Board approval was obtained. Results Ninety children less than 5 years of age were recruited after a written informed consent was obtained from parents or guardians. Forty-five subjects served as cases with ADD and 45 as controls. Thirty-six subjects out of 90 (40.0%) were positive for at least one enteropathogen, that is, 20 (44.4%) cases and 16 (35.5%) controls. Conclusions The three most common enteric pathogens were enteroaggregative E. coli (10.0%), Norovirus (6.7%), and Salmonella spp. (5.6%). The E. coli pathogens were 18.8% of all infections making them the most frequent pathogens. Half of ADD cases were negative for any pathogens.

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La enfermedad diarreica aguda: un reto para la salud pública en Colombia

Abstract: The ineffectiveness of health promotion and disease prevention activities in a population with easy access to health services set the stage for the appearance of cases of diarrhea with dehydration. Rotavirus infection plays an important role in the severity of ADD among Colombian children.

Cited by 16 publications

References 20 publications

Characterization of Shiga Toxin-ProducingEscherichia coliIsolated from Ground Beef Collected in Different Socioeconomic Strata Markets in Buenos Aires, Argentina

Characterization of Shiga Toxin-ProducingEscherichia coliIsolated from Ground Beef Collected in Different Socioeconomic Strata Markets in Buenos Aires, Argentina

Rotavirus G2P[4] Detection in Fresh Vegetables and Oysters in Mexico City

Case-Control Pilot Study on Acute Diarrheal Disease in a Geographically Defined Pediatric Population in a Middle Income Country

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