L1 mono- and polyclonal antibodies modify cell migration in early postnatal mouse cerebellum

Abstract

A major event of nervous system development is the migration of granule cell neurones, during the early postnatal development of the cerebellar cortex, from their germinating zone in the external granular layer to their final location in the internal granular layer. During migration, many granule cells are seen in direct cell-surface contact with processes of Bergmann glia, a subclass of astrocytes. In the neurological mutant mouse weaver, however, migration of granule cells is impaired, probably due to a deficit in cell-cell interactions. To gain insight into the cellular and molecular mechanisms involved in granule cell migration, we have used a modification of an in vitro assay system, previously described by Moonen et al., which displays migratory behaviour in small tissue explants during several days of suspension culture. The aim of this study was to investigate the process of granule cell migration by using antibodies directed against cell-surface components of developing neural cells. We report here that migration of 3H-thymidine-labelled granule cell neurones can be modified by Fab fragments of both mono- and polyclonal L1 antibodies, but not by Fab fragments of polyclonal antibodies prepared against mouse liver membranes, which also react with cerebellar cell surfaces.

Developmental regulation of gene expression for the MPTPδ isoforms in the central nervous system and the immune system

“…It has been reported that although N-CAM has five Ig-like domains, only the two most amino-terminal Ig-like domains are involved in the homophilic binding [30], that the migration of neurons in developing rat cerebellum is linked to the expression of another Ig superfamily of the cell adhesion molecule, Ng-CAM [31,32], and that in Drosophila, three molecules of the Ig superfamily, namely fasciclin II, fasciclin III, and neuroglican, are expressed not only during neurogenesis but also after the nervous system is completely established [33]. Thus, it may be that MPTP$ with three Ig-like domains are required for the migration of neurons or the synapse formation during neurogenesis.…”

Characterization of Ethanol‐Sensitive and Insensitive Fibroblast Cell Lines Expressing Human L1

“…Fab fragments of anti-Li rabbit antiserum were provided by Drs. Vance Lemmon (Fab-i) and Fritz Rathjen (Fab-2) (Lindner et al, 1983). mAb H28.123 was obtained from Immunotech (Marseille, France).…”

Regulation of axonal growth in the vertebrate nervous system by interactions between glycoproteins belonging to two subgroups of the immunoglobulin superfamily.

“…In brief, features in common to both proteins include (a) their gross domain structural organization (Moos et al, 1988;Burgoon et al, 1991); (b) their appearance as a pattern of polypeptides probably originated by partial proteolytic cleavage of a single precursor molecule (Grumet et al, 1984;Wolffet al, 1987;Sadoul et al, 1988); (c) the occurrence of a phosphorylation site on the cytoplasmic domain (Grumet et al, 1984;Faissner et al, 1985;Sadoul et al, 1989); (d) the tissue expression patterns in most areas of the nervous system on which data are available for corresponding developmental stages (Rathjen and Schachner, 1984;Stalleup et al, 1985;Daniloff et al, 1986;but ef. Shiga et al, 1990, for an exception); and (e) involvement in biological functions, such as neuronal migration (Lindner et al, 1983;Hoffman et al, 1986), promotion of neurite outgrowth (Lagenaur and Lemmon, 1987;Lemmon et al, 1989;Chang et al, 1990;Kadmon et al, 1990), and fasciculation (Hoffman et al, 1986;Fischer et al, 1986;Rathjen et al, 1987a). The argumentation that L1 and Ng-CAM may not be species homologues but relatives derived main has a three-dimensional structure similar to that of the immunoglobulin fold of the C2 subcategory.…”